LOCUS       sequence-433772-        9249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of pLEX302_circSMAD2(2,7)_OE expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Liu L, Neve M, Perlaza-Jimenez L, Xi X, Purcell J, Hawdon A, Conn 
            SJ, Zenker J, Tamayo P, Goodall GJ, Rosenbluh J
  TITLE     Systematic loss-of-function screens identify pathway-specific 
            functional circular RNAs.
  JOURNAL   Nat Cell Biol. 2024 Aug;26(8):1359-1372. doi: 
            10.1038/s41556-024-01467-y. Epub 2024 Aug 2.
  PUBMED    39095657
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-024-01467-y"; journalName: "Nat Cell Biol"; date: 
            "2024-08"; volume: "26"; issue: "8"; pages: "1359-1372"
COMMENT     Sequence Label: pLEX302_circSMAD2_OE
FEATURES             Location/Qualifiers
     source          1..9249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    611..736
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             783..963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(964..1190)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1218..1238)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1218..1236)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1257..1273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1257..1273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1270..1292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1281..1297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1305..1311,1312..1329,1330..1335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1350..1371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1488..1505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1659..2247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1739..1758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2418..3209,3210..3278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3041..3060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3279..3383)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3409..3864)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3546..3567)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3758..3777
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3991..4013
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4005..4022
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4006..4022
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4032..4051
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4032..4050
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      4071..4206
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4246..4367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4257..4276)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4311..4330
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4445..4678
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4750..5338
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4856..4867
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5265..5285
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5380..5421)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    5423..5447
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5450..5674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(6779..6803)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6826..7029)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6855..6875)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7030..7333
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7450..7567
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7609..8208)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7692..7712)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8189..8208
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8230..8730)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8293..8311)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(8905..8949)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(9134..9249,1..118)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
       61 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
      121 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
      181 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
      241 atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc ttacttttgt
      301 tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat
      361 gcacacaata gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct
      421 gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc
      481 caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat
      541 attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc
      601 gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt
      661 ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag
      721 tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc
      781 actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
      841 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
      901 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
      961 cccgtttatg tatcgagcta ggcacttaaa tacaatatct ctgcaatgcg gcaattcagt
     1021 ggttcgtcca atccatgtca gacccgtctg ttgccttcct aataaggcac gatcgtacca
     1081 ccttacttcc accaatcggc atgcacggtg ctttttctct ccttgtaagg catgttgcta
     1141 actcatcgtt accatgttgc aagactacaa gagtattgca taagactaca ttaagcttgc
     1201 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
     1261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     1321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     1381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     1441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     1501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     2341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     2401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     2461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     2521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     2581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     3301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     3361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     3421 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3481 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3541 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3601 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     3661 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     3721 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3781 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3841 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     3901 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     3961 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     4021 gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacgtcccta ggcctccaaa
     4081 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     4141 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     4201 cgggatgggc ggagttaggg gcgggatagc tagagccaga catgataaga tacattgatg
     4261 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     4321 atgctattgc tttatttgta accattataa gctgcaataa acaagttcct ctcactctct
     4381 gatattcatt tctttgcaag ttataaatac tgaataataa gatgacatga actactatac
     4441 gtactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     4501 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     4561 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     4621 gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag cccttccagt
     4681 cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt
     4741 aaagtcgacg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa
     4801 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga
     4861 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga
     4921 gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca gtgcccaaca
     4981 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag
     5041 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag
     5101 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca
     5161 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac
     5221 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag
     5281 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt
     5341 cgacttaacg cgccaccggt tagcgctagc tcattactac gtagaatcga gaccgaggag
     5401 agggttaggg ataggcttac caaccacttt gtacaagaaa gttgggggtc catagagccc
     5461 accgcatccc cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc
     5521 accccacccc ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt
     5581 tattaggaaa ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg
     5641 caaacaacag atggctggca actagaaggc acagtgctgg gattacaggt gtgagctacc
     5701 gtgccgagcc taattctttt ccttgcttct tacgttcgat acaagaggct gttttcctag
     5761 cgtggcttgc tgcctttggt aagaacatgt cgtccatctt gccattcacg ccgccagttg
     5821 tgaagagact gctgggatgg aagaagtcag ctggtgggtc tggaggagca ggcggaggag
     5881 agcagaatgg gcaggaagaa aagtggtgtg agaaagcagt gaaaagtctg gtgaagaagc
     5941 taaagaaaac aggacgatta gatgagcttg agaaagccat caccactcaa aactgtaata
     6001 ctaaatgtgt taccatacca agatattttc tgttgatcta cttttgagtt tactgatcct
     6061 gtttttgtgc tgtattcagt ctgttaagcc tacggtatct tccttctttt ttcaaactct
     6121 ctggcagccc caaatgtgtc ctctgattct agcacttgct ctgaaatttg gggactgagt
     6181 acaccaaata cgatagatca gtgggataca acaggccttt acagcttctc tgaacaaacc
     6241 aggtctcttg atggtcgtct ccaggtatcc catcgaaaag gattgccaca tgttatatat
     6301 tgccgattat ggcgctggcc tgatcttcac agtcatcatg aactcaaggc aattgaaaac
     6361 tgcgaatatg cttttaatct taaaaaggat gaagtatgtg taaaccctta ccactatcag
     6421 agagttgaga caccagtttt gcctccagta ttagtgcccc gacacaccga gatcctaaca
     6481 gaacttccgc ctctggatga ctatactcac tccattccag aaaacactaa cttcccagca
     6541 ggaattgagc cacagagtaa ttatattcca gtgactttaa tcaaagtact gtgagaagca
     6601 gaaaagaaaa gatcttaaaa cgccacctcc tggatatatc agtgaagatg gagaaacaag
     6661 tgaccaacag ttgaatcaaa gtatggacac agctgaagta taaaaaaaaa gtcattagta
     6721 cgtaccttta caaaaagtgg gccgggggtg gtggctcacg cctgtaatct cagcactttg
     6781 ccaacttttt tgtacaaact tgttgatccc gacagttagc cagagagctc tgcttatata
     6841 gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
     6901 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
     6961 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
     7021 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     7081 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     7141 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     7201 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     7261 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
     7321 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
     7381 ctattaataa ccccgggccc cctcgagccg gcgccaaagt ggatctctgc tgtccctgta
     7441 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     7501 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     7561 tttaaaattg tggatgaata ctgccatttg tctcaagatc tagaattctc aggcaccggg
     7621 cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt
     7681 gaagccgagc cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg
     7741 caccccggcg cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt
     7801 gccctggtgg tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg
     7861 gtgcggcgcc aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc
     7921 ggccatgcgc gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt
     7981 ccagaccgcc acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac
     8041 gcgcgtgagg aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac
     8101 ggtgtggcgc gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg
     8161 gacgtcgtcg cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg taagctccgg
     8221 tggtaccccc tggggagaga ggtcggtgat tcggtcaacg agggagccga ctgccgacgt
     8281 gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg cccacactac
     8341 cgccccacac cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc
     8401 tgagcagccg ctattggcca cagcccatcg cggtcggcgc gctgccattg ctccctggcg
     8461 ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc
     8521 cgcgaaccgc aaggaacctt cccgacttag gggcggagca ggaagcgtcg ccggggggcc
     8581 cacaagggta gcggcgaaga tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga
     8641 cccagggtcg gcgccgctgc gtttcccgga accacgccca gagcagccgc gtccctgcgc
     8701 aaacccaggg ctgccttgga aaaggcgcaa gggtcccctc ggggttggga ggtgggtctg
     8761 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     8821 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     8881 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     8941 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     9001 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     9061 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     9121 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     9181 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     9241 cctcagcaa
//
