LOCUS       sequence-433784-        6216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector for knocking in an N-terminal tag (FKBP12F36V and V5) for
            human PRRX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Kim S, Morgunova E, Naqvi S, Goovaerts S, Bader M, Koska M, Popov A,
            Luong C, Pogson A, Swigut T, Claes P, Taipale J, Wysocka J
  TITLE     DNA-guided transcription factor cooperativity shapes face and limb 
            mesenchyme.
  JOURNAL   Cell. 2024 Feb 1;187(3):692-711.e26. doi: 
            10.1016/j.cell.2023.12.032. Epub 2024 Jan 22.
  PUBMED    38262408
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.12.032"; journalName: "Cell"; date: "2024-02-1-
            1"; volume: "187"; issue: "3"; pages: "692-711.e26"
COMMENT     Sequence Label: pAAV_FKBP-V5-PRRX1
FEATURES             Location/Qualifiers
     source          1..6216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   826..955
                     /label=AAV2 ITR
     primer_bind     complement(1011..1030)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             join(2003..2005,2006..2326)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             2342..2383
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     repeat_region   3321..3450
                     /label=AAV2 ITR
     rep_origin      3821..4334
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3908..3927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4118..4139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4816..4835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4935..4957
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4995..5013)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5081..5185
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5186..5254,5255..6046)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5404..5423)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg cgctcgctcg
      841 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
      901 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag
      961 ttaatgatta acccgccatg ctacttatct acgtagccat gctctagcgg cctcggcctc
     1021 tgcataaata aaaaaaatta gtcagccatg agcttggccc attgcatacg ttgtatccat
     1081 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     1141 tattgactag gtgccagaga gcttcttgat ccaactgaga aggaaaaagg agcccagcaa
     1201 gaagaggggg agagagagaa gggaaagggg ggaacccacc agcaccctcc gtcggactct
     1261 tgaagccttt ttttttaatt cttaattttt ttttttactc tttacaaaaa gtaaagtgag
     1321 aatcctgctc tctaatacat ctgcaagaca tcaccctctc ctcctgaaac tttagtcact
     1381 cctgagaatc cacaggagtg cagagagggg ggaacacgtt ttcttgaaga tgttttaaag
     1441 ctggaacaag ccttcttctg ttggtgcttg aactcttgcc tgggaataac ttttttaacc
     1501 tttaaaaaaa ccattcactt tgattcttct ctcccacccc ttcttctctc ttcttctgtt
     1561 tgcctaactc ccccgccctg ctggcctccg ctttcctctc tcccccttgt tattattttt
     1621 agtctgtgcg tgtggacact tttggagagt tggaagggat ttttttctcc tgacttgaac
     1681 atagggtgac tttttaatat tgtattttag tgtggattat ctctttggac cgcgccggac
     1741 ttggcctcag gaaagcaacc aaggctgcgg aaggcggctg gtgcagaacg ctctgctcta
     1801 cagaaggggg tcccccgccc tcttttccaa tttttttttt tggccttcct ctccttccct
     1861 ccctcttcct ccctctctct ctctctctct ccactacccc cctctttctt ccccactcgg
     1921 ctcctctccc ccctcgcgcc cacagcgttt ggtgttgatt cgagcgggaa gaggggggtg
     1981 ggtgggatcg gtgggggaga ccatgggagt gcaggtggaa accatctccc caggagacgg
     2041 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga
     2101 tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa
     2161 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc
     2221 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc
     2281 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaatctg gatccggttc
     2341 cggcaagccc atccccaacc ccctgctggg cctggacagc acctccggtt caggctctgg
     2401 atccggttcc acctccagct acgggcacgt tctggagcgg caaccggcgc tgggcggccg
     2461 cttggacagc ccgggcaacc tcgacaccct gcaggcgaaa aagaacttct ccgtcagtca
     2521 cctgctagac ctggaggaag ccggggacat ggtggcggca caggcggatg agaacgtggg
     2581 cgaggctggc cggagcctgc tggagtcgcc gggactcacc agcggcagcg acaccccgca
     2641 gcaggacagt gagtgagggg cgcatgccca cgggggtgtg tgcccgggac agagggcggg
     2701 gacccgtgta gggcagctag agcccgtccg cggccagaaa gacaaggtcc tgggaccagg
     2761 agaggtgatg gcagacttca aaggaagggc cagtcacagg ggaaaccaga tgagaaaagc
     2821 gggtctcggc cgagggcata gccaaggagg acgctggaga gaggctccaa ggtccgggga
     2881 cactccgagg ccctgcggtc tcaggcaggg actctgtccg cggcggactc tctttacttc
     2941 ggccaggcac cggctcggtt catcccgcac gcggggcggc ggggctgggc tgggaaaagg
     3001 tcttagcggg gtgcaaactc agagagccag cgctgtttgg gaaagcagca ggttaaagat
     3061 gctgagtcgg gttggaaggg cttctctcgg gcagcgtatg acggcttgag ggagggcacc
     3121 aggcacaggg agcaggactt ggcgctgctc aggctgcact ctccacgcgg gcaccgtaag
     3181 gccgggcgcg accgggaggc cccgagagaa cgcccggccc cagacgctgg agctgctctc
     3241 tgccccctgc tgttgcctct accgcaaact agagcatggc tacgtagaca agtagcatgg
     3301 cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg
     3361 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     3421 ggcggcctca gtgagcgagc gagcgcgcag ctggcgtaat agcgaagagg cccgcaccga
     3481 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgattccgtt gcaatggctg
     3541 gcggtaatat tgttctggat attaccagca aggccgatag tttgagttct tctactcagg
     3601 caagtgatgt tattactaat caaagaagta ttgcgacaac ggttaatttg cgtgatggac
     3661 agactctttt actcggtggc ctcactgatt ataaaaacac ttctcaggat tctggcgtac
     3721 cgttcctgtc taaaatccct ttaatcggcc tcctgtttag ctcccgctct gattctaacg
     3781 aggaaagcac gttatacgtg ctcgtcaaag caaccatagt acgcgccctg tagcggcgca
     3841 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3901 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3961 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4021 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4081 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4141 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4201 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4261 ttaacgctta caatttaaat atttgcttat acaatcttcc tgtttttggg gcttttctga
     4321 ttatcaaccg gggtacatat gattgacatg ctagttttac gattaccgtt catcgattct
     4381 cttgtttgct ccagactctc aggcaatgac ctgatagcct ttgtagagac ctctcaaaaa
     4441 tagctaccct ctccggcatg aatttatcag ctagaacggt tgaatatcat attgatggtg
     4501 atttgactgt ctccggcctt tctcacccgt ttgaatcttt acctacacat tactcaggca
     4561 ttgcatttaa aatatatgag ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt
     4621 ctcccgcaaa agtattacag ggtcataatg tttttggtac aaccgattta gctttatgct
     4681 ctgaggcttt attgcttaat tttgctaatt ctttgccttg cctgtatgat ttattggatg
     4741 ttggaatcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     4801 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     4861 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4921 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4981 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5041 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5101 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5161 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5221 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5281 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5341 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5401 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5461 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5521 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5581 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5641 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5701 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5761 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5821 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5881 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5941 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6001 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6061 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6121 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6181 agcgtcagac cccgtagaaa agatcaaagg atcttc
//
