LOCUS       sequence-433839-        7827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transient expression of At BGLU10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7827)
  AUTHORS   Grutzner R, Konig K, Horn C, Engler C, Laub A, Vogt T, Marillonnet S
  TITLE     A transient expression tool box for anthocyanin biosynthesis in 
            Nicotiana benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Dec 20. doi: 10.1111/pbi.14261.
  PUBMED    38124296
REFERENCE   2  (bases 1 to 7827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Dec 20. doi: 10.1111/pbi.14261."
COMMENT     Sequence Label: pAGM13451
FEATURES             Location/Qualifiers
     source          1..7827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1237..1261
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(1417..2565)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(2901..3489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2981..3000)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3660..4451,4452..4520))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4283..4302
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4521..4625)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4712..5343
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    5435..5459
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        5645..5990
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     5933..5953
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      7627..7827
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
ORIGIN
        1 cgctactatt gtcttccact gggtgtcaaa ggagtgtcag ttaattaaag agtgtcgggg
       61 ccgacactct taatggaagt aaaagtgtgg gttttgctgc accgacactc ttaatttaag
      121 agtgtcgggg tcccgatgaa accgacgctt ttaatttaag agtgtgggtt tttccacacc
      181 gacactctta tgaatgttac cctaaattcc ccaatcctat actgtataag agctcatttt
      241 tacaacaatt accaacaaca acaaacaaca aacaacatta caattacatt tacaattatc
      301 gatacaatgt ctcctgaaag aaggcctgct gatattagaa gggctactga ggctgatatg
      361 cctgctgtgt gcactattgt gaatcattac attgagactt ctactgtgaa tttcaggact
      421 gagcctcaag aaccacaaga gtggactgat gatcttgtga ggcttagaga gaggtatcct
      481 tggcttgttg ctgaagtgga tggtgaagtg gctggtattg cttatgctgg tccttggaag
      541 gctaggaatg cttacgattg gactgctgag tctactgttt acgtgtctcc taggcatcaa
      601 agaactggtc ttggatctac tctttacact catcttttga agtctttgga ggctcagggt
      661 ttcaagtctg tggttgctgt gattggactt cctaatgatc cttctgtgag gatgcatgag
      721 gctcttggtt atgctcctag gggaatgctt agagctgctg gtttcaaaca tggaaattgg
      781 catgatgtgg gattctggca gcttgatttc tctcttcctg ttcctcctag acctgttctt
      841 cctgtgactg agatttgagc ttactccacc taaatactat gccggttcaa ataagaaagt
      901 aatgattatg tacccaaata atacatttat aagatgtatg tgtgtgatgg acatgtttct
      961 gtatcgtacc aaaactaaat aggtggcttt tatcccaaaa aagtgctttc gtttgatgac
     1021 cttatgattt ctaaagagtg ttgttgttgc tgcttatgtt taataaaagt gatgtatatt
     1081 atcagttgat ttccccacgt ggaaggggtg attatattgc acaaaattaa gttaaaagta
     1141 acattgtggt gtaataaaaa ccctagtgaa tttataacct ataagtaagt ggtgcagtag
     1201 tgaatttatc acgaagtggt ttaaactatc agtgtttgac aggatatatt ggcgggtaaa
     1261 cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt gaaaaggttt
     1321 atccgttcgt ccatttgtat gtgcatgcca accacagggt tccccagatc aggcgctggc
     1381 tgctgaaccc ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc
     1441 atcattgacc caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc
     1501 gacctgctcg cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt
     1561 ttccagcttg agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc
     1621 cgtcggcgac agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa
     1681 cagcacgacg atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc
     1741 caggacgcgg aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt
     1801 gaagcccatc gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg
     1861 gccattgatc gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc
     1921 gccgataggg gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc
     1981 ggcccgcagc tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac
     2041 cttgttttgc agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg
     2101 ggccgtgtcg tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga
     2161 aagctgcatt tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc
     2221 gctgacctgt tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc
     2281 tcgcgtgtcg atggtcatcg acttcgccaa acctgccgcc tcctgttcaa gacgacgcga
     2341 acgctccacg gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg
     2401 ctcgatcttg gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg
     2461 cgcacgcatg acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc
     2521 gatcagttct tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg
     2581 gattgccccg actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc
     2641 cttggtgtcc agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc
     2701 gtccttctcg tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc
     2761 caaatacttg ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt
     2821 gcgctcctgc ttgtcgccgg catcgttgcg ccacatctag gatctgccag gaaccgtaaa
     2881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     3001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     3061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     3121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     3181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     3241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3301 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3481 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3781 agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac
     3841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3961 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     4021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     4081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     4141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     4201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     4261 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     4321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcaaggt
     4561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4621 ccgcgcacga attggccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
     4681 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
     4741 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
     4801 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
     4861 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
     4921 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
     4981 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
     5041 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
     5101 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
     5161 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
     5221 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
     5281 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
     5341 gtcgcaaagg agatcctgat ctgactgatg ggctgcctgt atcgagtggt gattttgtgc
     5401 cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca
     5461 aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac tggggtggat
     5521 gcagtgggcc ccactctgtg aagacaagca agaattcaag cttggaggtc aacatggtgg
     5581 agcacgacac tctggtctac tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg
     5641 ctattgagac ttttcaacaa aggataattt cgggaaacct cctcggattc cattgcccag
     5701 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
     5761 attgcgataa aggaaaggct atcattcaag atctctctgc cgacagtggt cccaaagatg
     5821 gacccccacc cacgaggagc atcgtggaaa aagaagaggt tccaaccacg tctacaaagc
     5881 aagtggattg atgtgacatc tccactgacg taagggatga cgcacaatcc cactatcctt
     5941 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctcgagta
     6001 taagagctca tttttacaac aattaccaac aacaacaaac aacaaacaac attacaatta
     6061 catttacaat tatcgataca atgaaacttt actctctact ttccgttttc ttggtcatcc
     6121 tcttggcaac aagcgacagt gatgccttta ccagaaacaa ttttccaaag gatttcctct
     6181 ttggagccgc cacttctgct tatcagtggg aaggagctgt tgctgaagat ggaagaactc
     6241 ctagcgtctg ggatactttc tcacacactt acaatagagg taatttaggt aatggagata
     6301 taacatccga tgggtatcac aaatacaagg aagatgttaa gctgatggca gaaatgggct
     6361 tagaatcatt cagattctct atctcctggt caagacttat acctaatgga aggggactca
     6421 ttaacccaaa aggtctactg ttttacaaga acctcatcaa agaactaata agccatggaa
     6481 tcgaaccaca tgttacactt taccactatg atcttcctca gtctcttgaa gatgagtatg
     6541 gaggctggat caaccgcaaa atcatagagg acttcactgc ttatgcagat gtatgtttca
     6601 gagagtttgg ggaggatgtg aagttatgga ctacaataaa cgaagctaca attttcgcca
     6661 ttggttctta tgaccaagga atctcgcccc ctggacattg ttctcctaac aaattcatca
     6721 attgtactag tggaaattct tctacagaac catatcttgc aggccataac atattgctag
     6781 ctcatgcctc tgcttcaaag ttgtataaac taaagtacaa gagtacgcag aaaggatcca
     6841 tagggcttag tatatttgca ttcgggttat ctccatatac aaactccaag gatgatgaaa
     6901 tcgcaactca aagagctaaa gctttcttct atggctggat gttgaagcct ttggtatttg
     6961 gagactatcc ggatgaaatg aagagaactg ttggatcgag attaccggtg ttttcggagg
     7021 aagagtcaga gcaacttaaa ggatcatctg actttatagg aattattcac tacacgacat
     7081 tttatgtcac aaacaaaccc tcaccttcta tctttcccag catgaatgaa ggcttcttta
     7141 aagacatggg agtatatatg atttccgctg cgaattcttc atttcttctg tgggaggcta
     7201 ctccatgggg tcttgaaggt attcttgagt atataaagca gagctataac aatcctccaa
     7261 tctatattct tgaaaatggt atgccgatgg gacgcgactc gacattacaa gacacacaaa
     7321 gaattgaatt cattcaagct tacattggtg ctatgctcaa tgccatcaag aatggatcgg
     7381 acacgagagg ttactttgta tggtcgatga ttgatttgta tgagttactg agtggatata
     7441 cgaccagctt cggaatgtac tatgtgaatt tcagcgatcc tggtcgcaag aggactccaa
     7501 agctctctgc ttcttggtac actggtttcc tcaatggtac aattgatgtt gctactcaag
     7561 atactattca gttgcagagc aacatctctg gctcttcttc attgtaagct tgatcccccg
     7621 tcgacagcta gctatatcat caatttatgt attacacata atatcgcact cagtctttca
     7681 tctacggcaa tgtaccagct gatataatca gttattgaaa tatttctgaa tttaaacttg
     7741 catcaataaa tttatgtttt tgcttggact ataatacctg acttgttatt ttatcaataa
     7801 atatttaaac tatatttctt tcaagat
//
