LOCUS       sequence-433841-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of PM-miniTurbo (plasma membrane control) 
            tagged with C-terminal V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  TITLE     Gateway destination clones for generating protein-proximity networks
            using proximity-dependent biotinylation proteomics
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-PM-miniTurbo-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     16..35
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             104..703
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(104..123)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     600..620
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             841..1074
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1146..1267
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1183..1202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1237..1256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1307..1442
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1462..1481)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1463..1481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1491..1508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1491..1507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1500..1522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1649..2104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1736..1755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1946..1967
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2130..2234
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2235..2303,2304..3095)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3266..3854
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3755..3774
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4008..4025
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4142..4163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4178..4183,4184..4201,4202..4208)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4216..4232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4221..4243
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4240..4256
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4240..4256
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4275..4295
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4277..4295
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4323..4549
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4550..4730
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4777..4902
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5395..5628
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5813..5857
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6124..6240
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    6274..6294
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6294..7505
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7342..7361
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    7506..7530
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7569..8339
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             8346..8387
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(8392..8416)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8446..9034
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8499..8519)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8917..8928)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        join(9048..9464,1..83)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9083..9106)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
       61 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
      121 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
      181 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
      241 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
      301 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
      361 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
      421 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
      481 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
      541 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
      601 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
      661 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
      721 gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa
      781 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
      841 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      901 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      961 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     1021 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     1081 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     1141 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1201 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1261 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     1321 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     1381 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     1441 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     1501 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     1561 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     1621 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     1681 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1741 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1801 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1861 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1921 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1981 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2041 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     2101 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2161 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2221 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     2281 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     2341 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     2401 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     2461 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2521 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2581 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2641 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2701 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2761 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2821 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2881 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2941 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     3001 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     3061 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     3121 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     3181 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     3241 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     3301 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     3361 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     3421 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3481 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3541 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3601 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3661 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3721 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3781 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3841 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3901 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3961 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     4021 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     4081 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     4141 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     4201 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     4261 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     4321 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     4381 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     4441 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     4501 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     4561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     4741 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     4801 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     4861 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     4921 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     4981 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     5041 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     5101 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     5161 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     5221 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     5281 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     5341 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     5401 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     5461 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     5521 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     5581 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     5641 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     5701 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     5761 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     5821 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     5881 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     5941 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     6001 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     6061 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     6121 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     6181 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
     6241 atcgtccagt gtggtggaat tctgcagata tcacaacttt gtatagaaaa gttggcctcc
     6301 gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc
     6361 agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga cagcggcccg
     6421 ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt taggacggga
     6481 cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg aggaaaagta
     6541 gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc gatgattata
     6601 taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt
     6661 cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg gctggccggg
     6721 gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac cgccaagggc
     6781 tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga gcgcagcaaa
     6841 atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt
     6901 gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc cttcgctaat
     6961 gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac cctgacgtga
     7021 agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc agttatggcg
     7081 gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt
     7141 cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt gtgcggtagg
     7201 cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg aatcgacagg
     7261 cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg tcggttttat
     7321 gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg gggttggcga
     7381 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
     7441 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
     7501 tagacgcaac tttgtacaaa aaagcaggct taatgggatg tataaaatca aaagggaaag
     7561 acagcgcgat cccgctgctg aacgctaaac agattctggg acagctggac ggcgggagcg
     7621 tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga atcggcgagc
     7681 tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga agcagaggga
     7741 ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg cgcctgaagc
     7801 ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc
     7861 gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat ctgcaggata
     7921 gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct gcacagatcg
     7981 tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg
     8041 gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg gccgctatgc
     8101 tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg gctccatatc
     8161 tgtcacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg atcattgggg
     8221 acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg ctgctggaac
     8281 aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct gccgaaaagg
     8341 ctagcggcaa gcccatcccc aaccccctgc tgggcctgga cagcacctaa aacccagctt
     8401 tcttgtacaa agttgtgata tccagcacag tggcggccgc tcgacaatca acctctggat
     8461 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8521 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8581 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8641 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     8701 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     8761 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     8821 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     8881 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     8941 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     9001 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttctaccggg taggggaggc
     9061 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     9121 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     9181 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     9241 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     9301 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     9361 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     9421 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggt
//
