LOCUS       sequence-433869-        9180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human IFIT5_WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9180)
  AUTHORS   Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, 
            Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar
            S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, 
            Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne 
            Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M
  TITLE     Human protein interaction networks of ancestral and variant 
            SARS-CoV-2 in organ-specific cells and bodily fluids.
  JOURNAL   Nat Commun. 2025 Jul 1;16(1):5784. doi: 10.1038/s41467-025-60949-1.
  PUBMED    40593736
REFERENCE   2  (bases 1 to 9180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-1"; volume: "16(1):5784. doi"; pages: " 
            10.1038/s41467-025-60949-1"
COMMENT     Sequence Label: pLD-puro-Cc-IFIT5_WT-VA
FEATURES             Location/Qualifiers
     source          1..9180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             419..463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     761..781
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        1070..1273
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1224..1244
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1270..1294
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1325..1349
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2796..2820)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2900..2923
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2960..2983
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2996..3013
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3023..3043
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3056..3076
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             3089..3154
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3202..3319
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3368..3878
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3797..3815
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3900..4499
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3900..3919)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4396..4416
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4627..4860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4932..5053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4969..4988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5023..5042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5093..5228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5248..5267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5249..5267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5277..5294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5277..5293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5286..5308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5435..5890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5522..5541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5732..5753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5916..6020
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6021..6089,6090..6881)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6239..6258)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7052..7640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7541..7560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7794..7811
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7928..7949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7964..7969,7970..7987,7988..7994)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8002..8018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8007..8029
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8026..8042
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8026..8042
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8061..8081
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8063..8081
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8109..8335
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8336..8516
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8563..8688
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
       61 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
      121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
      181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
      241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
      301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
      361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
      421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
      481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
      541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
      601 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
      661 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
      721 tcgatcacga gactagcctc gagcggccgc ccccttcacc gagggcctat ttcccatgat
      781 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
      841 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      901 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      961 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1021 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1081 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1141 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1201 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1261 tataagcaga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgtac
     1321 gcgtacaagt ttgtacaaaa aagttggcat gagtgaaatt cgtaaggaca ccttgaaggc
     1381 cattctgttg gagttagaat gtcattttac atggaattta cttaaggaag acattgatct
     1441 gtttgaggta gaagatacaa ttgggcaaca gcttgaattt cttaccacaa aatctagact
     1501 tgctctttat aacctattgg cctatgtgaa acacctaaaa ggccaaaata aagacgccct
     1561 tgagtgcttg gaacaagcag aagaaataat ccagcaagaa cactcagaca aagaagaagt
     1621 acgaagcctg gtcacttggg gaaactatgc ctgggtgtat tatcacatgg accagcttga
     1681 agaagctcag aagtatacag gtaagatagg gaatgtctgt aagaaattgt ccagtccttc
     1741 taactacaag ttggagtgtc ctgagactga ctgtgagaaa ggctgggcac tcttgaaatt
     1801 tggaggaaag tattatcaaa aggctaaagc ggcttttgag aaggctctgg aagtggagcc
     1861 tgacaatcca gaatttaaca tcggctatgc tatcacagtg tatcggctgg atgattctga
     1921 tagagaaggg tctgtaaaga gcttttctct ggggcctttg agaaaggctg ttaccctgaa
     1981 cccagataac agctatatta aggtttttct ggcactgaag cttcaagatg tacatgcaga
     2041 agctgaaggg gaaaagtata ttgaagaaat cctggaccaa atatcatccc agccttacgt
     2101 ccttcgttat gcagccaagt tctataggag aaaaaattcc tggaacaaag ctctcgaact
     2161 tttaaaaaag gccttggagg tgacaccaac ttcttctttc ctgcatcacc agatgggact
     2221 ttgctacagg gcacaaatga tccaaatcaa gaaggccaca cacaacagac ctaaaggaaa
     2281 ggataaacta aaggttgatg agctgatttc atctgctata tttcatttca aagcagccat
     2341 ggaacgagac tctatgtttg catttgccta cacagacctg gccaacatgt atgctgaagg
     2401 aggccagtat agcaatgctg aggacatttt ccggaaagct cttcgtctgg agaacataac
     2461 cgatgatcac aaacatcaga tccattacca ctatggccgc tttcaggaat ttcaccgtaa
     2521 atcagaaaat actgccatcc atcattattt agaagcctta aaggtcaaag acagatcacc
     2581 ccttcgcacc aaactgacaa gtgctctgaa gaaattgtct accaagagac tttgtcacaa
     2641 tgctttagat gtgcagagtt taagtgccct agggtttgtt tacaagctgg aaggagaaaa
     2701 gaggcaagct gctgagtact atgagaaggc acaaaagata gatccagaaa atgcagaatt
     2761 cctgactgct ctctgtgagc tccgactttc catttaccca actttcttgt acaaagtggt
     2821 tgattctaga gcgatcgcca gagagctgtt caacctgctg ggcgagaacc agccccccgt
     2881 ggtgatcaag gcgatcgcct ggagccaccc tcagttcgag aagggaggag gaagcggcgg
     2941 aggcagcgga ggaggaagct ggagccaccc gcagttcgag aaaggcggaa gcggccatca
     3001 ccatcaccat caccctgcag gagaaaatct gtacttccag gggagaggag gcagcgagaa
     3061 cctgtacttt caggggcctg caggaagaga ctacaaagac catgacggtg attataaaga
     3121 tcatgacatc gattacaagg atgacgatga caagtagttc gaattctcga cctcgagaca
     3181 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     3241 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     3301 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg
     3361 gctcgagggg gttggggttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     3421 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     3481 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     3541 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     3601 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     3661 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     3721 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     3781 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     3841 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggatccaccg gagcttacca
     3901 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     3961 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4021 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4081 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4141 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4201 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     4261 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     4321 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     4381 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     4441 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     4501 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcggtac
     4561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4621 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     4681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     4741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     4801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     4861 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4921 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5041 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     5101 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5161 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5221 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     5281 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5341 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5401 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     5461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5641 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5761 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5821 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5881 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5941 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6001 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6061 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6121 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6181 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6241 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6301 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6361 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6421 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6481 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6541 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6601 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6661 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6721 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6781 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6841 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6901 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6961 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7021 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7081 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7141 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7201 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7261 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7321 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7381 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7441 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7501 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7561 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7621 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7681 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7741 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7801 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7921 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7981 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     8041 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     8101 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     8161 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     8221 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     8281 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     8341 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8401 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8461 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     8521 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     8581 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     8641 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8701 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     8761 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     8821 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8881 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8941 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     9001 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     9061 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9121 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
//
