LOCUS       sequence-433871-       13304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycyclin inducible expression of mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Wee K, Hediyeh-Zadeh S, Duszyc K, Verma S, N Nanavati B, Khare S, 
            Varma A, Daly RJ, Yap AS, Davis MJ, Budnar S
  TITLE     Snail induces epithelial cell extrusion by regulating RhoA 
            contractile signalling and cell-matrix adhesion.
  JOURNAL   J Cell Sci. 2020 Jul 6;133(13). pii: jcs.235622. doi: 
            10.1242/jcs.235622.
  PUBMED    32467325
REFERENCE   2  (bases 1 to 13304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-07-6"; volume: "133(13). pii: jcs.235622. doi"; 
            pages: " 10.1242/jcs.235622"
COMMENT     Sequence Label: pTripZ-Tet-ON mCherry
FEATURES             Location/Qualifiers
     source          1..13304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3007..3033
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3072..3646,3647..3649,3650..3658)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3665..4375
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3810..3828)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4078..4097
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     ncRNA           4397..4490
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           4516..4628
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(4723..4741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        4755..5265
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     5184..5202
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     protein_bind    complement(5278..5311)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             5318..6025
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(6026..6059)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    6082..6654
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6657..7259
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7156..7176
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7275..7863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7328..7348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7746..7757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8070..8303
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8404..8421)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8410..8634
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8680..9108
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8767..8786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8977..8998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9117..9137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9122..9451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9302..9437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9364..9383
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10659..10780
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10829..10845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10829..10845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10842..10864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10853..10869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10877..10883,10884..10901,10902..10907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10922..10943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11060..11077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11231..11819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11311..11330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11990..12781,12782..12850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12613..12632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12851..12955)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13003..13137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtgcc
     3001 accatgtacc catacgatgt gccagattac gctggtaccg gatccgtcga cctcgaggaa
     3061 ttcgcggccg ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     3121 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     3181 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     3241 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     3301 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     3361 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     3421 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     3481 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     3541 gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     3601 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccga
     3661 attcatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     3721 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3781 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3841 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3901 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3961 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     4021 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     4081 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     4141 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4201 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4261 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4321 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtgagttta
     4381 aacatcgata gtttgtttga atgaggcttc agtactttac agaatcgttg cctgcacatc
     4441 ttggaaacac ttgctgggat tacttcttca ggttaaccca acagaaggct cgaggtaacc
     4501 ggatcctgat cagaattcaa ggggctactt taggagcaat tatcttgttt actaaaactg
     4561 aataccttgc tatctctttg atacattttt acaaagctga attaaaatgg tataaattaa
     4621 atcacttttt tcaattggaa gactaatgcg gccggccatt actccgtctc gtgtcttgtt
     4681 gcatatgtct gctggtttgt ttgatgttgt ttgcgggcgg gccctatagt gagtcgtatt
     4741 acctaggacg cgtgggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     4801 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     4861 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     4921 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     4981 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     5041 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     5101 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     5161 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     5221 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggctag cggatccata
     5281 acttcgtata gtatacatta tacgaagtta tgccaccatg tctaggctgg acaagagcaa
     5341 agtcataaac ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag
     5401 gaaactcgct caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa
     5461 gcgggccctg ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg
     5521 ccccctggaa ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg
     5581 tgctctcctc tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa
     5641 acagtacgaa accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga
     5701 gaacgcactg tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga
     5761 acaggagcat caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc
     5821 acttctgaga caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt
     5881 cggcctggaa ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc
     5941 gaccgacgcc cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgattt
     6001 tgaccttgac atgctccccg ggtaaataac ttcgtatagt atacattata cgaagttatg
     6061 gatccgcggc cgcaaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     6121 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6181 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     6241 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     6301 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     6361 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     6421 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6481 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6541 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6601 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg
     6661 ccaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     6721 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     6781 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6841 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6901 gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt
     6961 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7021 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7081 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg
     7141 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7201 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag
     7261 ttcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7321 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7381 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7441 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7501 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7561 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7621 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7681 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7741 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7801 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7861 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     7921 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     7981 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     8041 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     8101 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     8161 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     8221 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8281 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     8341 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgtttaa acccgctgat
     8401 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8461 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8521 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8581 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8641 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     8701 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8761 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8821 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8881 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8941 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     9001 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     9061 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     9121 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     9181 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     9241 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9301 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9361 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     9421 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     9481 cggatctgat cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     9541 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     9601 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     9661 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     9721 tccggaagtg cttgacattg gggaattaat tcagcgagag cctgacctat tgcatctccc
     9781 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     9841 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     9901 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     9961 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
    10021 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
    10081 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
    10141 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
    10201 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
    10261 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
    10321 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
    10381 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
    10441 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
    10501 ccagcactcg tccgagggca aaggaatagc acgtgctacg agatttcgat tccaccgccg
    10561 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
    10621 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
    10681 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10741 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
    10801 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    10861 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10921 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    10981 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    11041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    11101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    11161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    11221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    11281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    11341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    11401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    11461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    11521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    11581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    11641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
    11701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    11761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    11941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    12001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    12061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    12121 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    12181 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    12241 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    12301 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    12361 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    12421 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    12481 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    12541 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    12601 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    12661 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    12721 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    12781 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    12841 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    12901 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    12961 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
    13021 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
    13081 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    13141 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    13201 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    13261 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
