LOCUS       sequence-433873-        3095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NanoLuc reporter plasmid to optimize chloroplast cell-free systems, 
            universal test construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Bohm CV, Inckemann R, Burgis M, Baumann J, Brinkmann CK, Lipinska 
            KE, Gilles S, Freudigmann J, Seiler VN, Clark LG, Jewett MC, Voll 
            LM, Niederholtmeyer H
  TITLE     Chloroplast Cell-Free Systems from Different Plant Species as a 
            Rapid Prototyping Platform.
  JOURNAL   ACS Synth Biol. 2024 Aug 16;13(8):2412-2424. doi: 
            10.1021/acssynbio.4c00117. Epub 2024 Jul 19.
  PUBMED    39028299
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.4c00117"; journalName: "ACS Synth Biol"; date: 
            "2024-08-16- 16"; volume: "13"; issue: "8"; pages: "2412-2424"
COMMENT     Sequence Label: pOP01-UTC-7.0
FEATURES             Location/Qualifiers
     source          1..3095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             486..508
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1332..2123,2124..2192))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1955..1974
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2193..2297)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2400..2988
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2889..2908
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacacgtctc gtactggacc aaaacgaaaa aaggccgctt tcgcggcctc ttttctggaa
       61 tttggtaccg agtcgacacc ctaccgtatc gctgtggcag gcacaggatc taaccggtcc
      121 tggaccggcg taagttgctg tacagccaaa tcttccgtac taattctaca ggatttgtca
      181 actgcaaacg aatttctagt cggaacggat cacgtgcacc ggggtatgcc gtggttgaat
      241 atcggcacat gaggggctcg tgggacactt ccagaactgt ctggcgtgcg aagtctctgc
      301 taaatgcaaa acagtcagga ctcgttacag ctcgtgtatg ggagctccgg gtaggccgat
      361 cgtcaattgc ggctcggtac caaattttcg aaaaaagacg ctgaaaagcg tcttttttcg
      421 ttttggtccg gagtaatacg actcactata gtactagacc acaacggttt ccctctagaa
      481 ataattttgt ttaactttaa gaaggagata tacataatgg ttttcacttt agaagacttc
      541 gtaggtgact ggcgtcaaac agcaggttat aatttagacc aagttttaga acaaggtggt
      601 gtatcaagtt tattccaaaa tttaggtgtt tctgttactc caattcaacg tatcgtatta
      661 agtggtgaaa atggtcttaa aattgacatc catgttatta ttccttatga aggtctttca
      721 ggtgaccaaa tgggtcaaat tgaaaaaatt tttaaagtag tttatcctgt agatgaccat
      781 cactttaaag taattttaca ctatggtact ttagtaattg atggcgttac acctaatatg
      841 attgactact ttggtcgtcc ttatgaaggt attgctgttt ttgatggtaa aaaaatcaca
      901 gttacaggta cattatggaa tggtaataaa attattgacg aacgtttaat caatcctgat
      961 ggttcattat tattccgtgt tacaattaat ggtgttacag gttggcgtct ttgtgaacgt
     1021 atccttgctg cttaaataat aaataacgga ttgtgtccgt aatcacacgt ggtgcgtacg
     1081 ataacgcata gtgtttttcc ctccacttaa atcgaagggt tgtgtcttgg atcgcgcggg
     1141 tcaaatgtat atggttcata tacatccgca ggcacgtaat aaagcgaggg gttcgaatcc
     1201 ccccgttacc cccggtaggg gcccacgctg cggccgcgat tatcaaaaag gatcttcacc
     1261 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1321 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     1381 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     1441 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     1501 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     1561 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     1621 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     1681 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     1741 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     1801 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     1861 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     1921 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     1981 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     2041 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     2101 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     2161 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     2221 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     2281 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct
     2341 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2401 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2461 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2521 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2581 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2641 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2701 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2761 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2821 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2881 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2941 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3001 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3061 ttatcccctg attctgtgga taaccgtgcg gccgc
//
