LOCUS       sequence-433880-        4916 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo visualization of actin cytoskeleton.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4916)
  TITLE     LifeAct Fluorescent Proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lifeact-Electra2
FEATURES             Location/Qualifiers
     source          1..4916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..51
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     polyA_signal    843..977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(867..886)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     921..940
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1090..1110)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1090..1108)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1129..1145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1129..1145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1142..1164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1153..1169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1177..1183,1184..1201,1202..1207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1360..1377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1531..2119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1611..1630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2290..3081,3082..3150))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2913..2932
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3151..3255)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3281..3736)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3418..3439)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3630..3649
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3807..4791
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        4826..4844
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4826..4843
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 atgggtgttg cagacctaat aaagaaattc gagtcaatct cgaaagagga gggttccgga
       61 ccatccggaa tggtgtctaa gggcgaagag ctgatcgagg aaaatatgcg tatgaaggtg
      121 gtcatggaag gttcggtcaa cggccaccaa ttcaaatgca caggtgaagg agaaggcaga
      181 ccgtacgagg gagtgcaaac catgaggatc aaagtcatcg agggaggacc cctgccattt
      241 gcctttgaca ttcttgccac gtcgttcctg ttcggcagca agacctttat caagtacccg
      301 gccgacatcc ctgatttctt cgaacagtcc tttcctgagg gctttacttg ggaaagagtt
      361 acgagatacg aagatggtgg agtagtcacc gtcacgcagg acaccagcct tgaggatggt
      421 gggctcgtct acaacgtcaa ggtcagaggg gtaaactttc actccaaggg tcccgtgatg
      481 cagaagaaga ccgagggttg ggagcctttc acagagatga tgtatccagc agatggtggt
      541 ctgagaggat ataccgacat cgcactgaaa gttgatggtg gtggccatct gcacgccaac
      601 atcgtgacaa cttacaggtc aaaaaagacc gtcgggaaca tcaagatgcc cggtgtccat
      661 gccgttgatt accgcctgga aaggatcgaa gagagtgaca atgaaaccta cgtagtgcta
      721 agagaagtgg cagttgccaa atacagcaac cttggtggtg gcatggacga gctgttcaag
      781 taacatcgat tggaattcaa ggcctctcga gcctctagaa ctatagtgag tcgtattacg
      841 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      901 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      961 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     1021 aggtgtggga ggttttttaa ttcgcggccg cggcgccaat gcattgggcc cggtacccag
     1081 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     1141 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg ggagcataaa
     1201 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1261 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1321 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2161 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2221 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2281 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2341 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2401 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2461 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2521 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2581 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2641 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2701 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     2761 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     2821 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     2881 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     2941 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3001 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3061 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3121 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3181 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3241 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     3301 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     3361 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     3421 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     3481 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     3541 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     3601 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     3661 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     3721 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     3781 gtgcgggcct cttcgctatt acgccagtcg accgccaatt caatatggcg tatatggact
     3841 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact
     3901 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac
     3961 catgccaatt caatatggcg gacctggcac tgtgccaact ggggaggggt ctacttggca
     4021 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca
     4081 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc
     4141 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg
     4201 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg
     4261 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt
     4321 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat
     4381 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt
     4441 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc
     4501 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa
     4561 tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg
     4621 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc
     4681 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc
     4741 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa ccgccattct
     4801 gcctggggac gtcggagcaa gcttgattta ggtgacacta tagaatacaa gctacttgtt
     4861 ctttttgcag gatccactag tggcgcgcca ttaattaaag gccggccagc gatcgc
//
