LOCUS       sequence-433881-        5672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L.variegatus polyubiquitin-C promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5672)
  TITLE     Stable germline transgenesis using the Minos Tc1/mariner element in 
            the sea urchin, Lytechinus pictus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LvPolyUb
FEATURES             Location/Qualifiers
     source          1..5672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(3508..3524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3508..3524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3521..3543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(3696..4284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3776..3795)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(4401..4425)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4525..5316,5317..5385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5148..5167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5386..5490)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5589..5611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5603..5620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5604..5620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttacta gtcctcacaa catttcttcc cttttttgcg ggagttgggg caaataatcc
       61 tgacaatgtc caatgattat tttaaaaagt gctatgtaca atcgctttgt atttgtataa
      121 ttgttataaa aaatgcattg tatgtattta tatttacata cataattatt ctacaaagtg
      181 tattgtacaa tcactttata tcaactttag atgtatcgtg ttattagtgt caaatctgac
      241 tgcgtgcata ctgattcgat gccaaaagtg gattaatgcc tccttgtgcc ccgcgtgtct
      301 cttggcaact agagcattat tgtagaatat tggggcacct aaaatacact gacaagcaat
      361 catccaaaaa tatctaacaa aatactaaac aataacagga cggtcatttt tctgatatct
      421 aaaacaagtt tttgttgaag ggacattgtg caagactttc aaaaacttat taagaatgtt
      481 gaaccgttta ctttttttat tttaacacgt gatactatag tatacataac gattgggcga
      541 aaaaataccg tgacagtcaa tcaaacaatc taaattccca ctttgcagaa tcacttttgt
      601 gattcccttg tatcttcaac aagatataga tagatggata gcatggaagt ttttgttttt
      661 tacttagaaa tggtaaaacg atgttttttc cccagggtct tttcccccta tgtaaattat
      721 gtttcattct ttcaataaaa aaaaacacaa actgaaaaaa aacttataag caaaaaaaaa
      781 ggcgtaaata tttttttatg aactctgtta aacggaaaca acaacaacac agtcgccaat
      841 atatacctcc cgcgaccagt gatcattcca gaaaatgttt tgggccgaaa ttatattgca
      901 taattccgaa attgcgagtc atcatcgtct gcaatatctg gcgcgggtta atatgacgtc
      961 atgtgccata gatacacaat ctatctttat tacccaatag aaaaggatga aatagtccat
     1021 gtgacacaga ctgaccaatc gcaagtcaac acaaagcacc atataaaaca cacgcaagcg
     1081 tcgcagtgtc aataaattgc atgagacttc cgcagaggtt gcactcgaga agaactcgtc
     1141 catttttttc cagaaaaact ctaatttcaa agtaagtaga cgaggttttc acattatttt
     1201 aaggcagtta tttcaatgaa gatgcagttt gtaaatgttt gatttaaaaa aagcactttt
     1261 gaagaagaat gatgatgaag aacataaatt gtcaacgacc acatcgtcgt cattattcgt
     1321 gtgctttttt tttgttgtaa tttatgtttg gctttccaaa tgaaggcaat ataggaacat
     1381 taacagcatg aacaaagtct catatttagt ttgattgaaa gttcaaatta tagggcgtag
     1441 aaatcattga aattgtaggg tggataccgt ggattattgc gtcgtgtgtg tgcatgataa
     1501 gatcgtctga ctcagacgtc gcgtcttttg ttgtgtggtg tgtgtggtat ggatgatgat
     1561 caattgtgat gtttggtggg gattttcatg aacgctgaat taaaagacaa tgagtggaaa
     1621 tggccatgcc aatttctggt ccatggattg tgtgaatgca atgacaattt cattcaattt
     1681 ttgtctgggc tgccatgatg aacatgatga agtctgaact gaagtgttgt gattgaaatg
     1741 acaaagaatt tttgtcatga gaaagaatga ggcatgtacg gtacggtaac ttaaaacgtt
     1801 gatagctagg tgtagatttt ctagatacgt ttggcagtag gcctagatct agaggcactt
     1861 ggccaatcat gaatagtcta tgggagacaa tttcccatat tgaagacttg ctttgcaaaa
     1921 gaaacgacac caacaaagcg taattttttc caccccaaat tttttcgcga aggtcagaac
     1981 agaagcccat ggcaaaggcc tgttttgtat gtgcatggcc atggatgaaa aaaatataaa
     2041 tacaaaagtt ggtgaatttt taaagatgaa aaggggtaat ttttcatgag gtatctgctt
     2101 aggccctatc acatttttat ttgctgccaa ggtaattctt ttgctgcatg tgtaaaaaaa
     2161 ggaaattggt ctcccaaact gggagtggag actgtctctg aaattggata cccaagttct
     2221 ttaatcttta caaaattctg atattgaaaa taatctctct catgggagat gttctcccat
     2281 attggctgtg caccagttgg tcggtgtgca aagtctagac aatacgtatc caggcactat
     2341 cgcctatgaa taatgcaata gcagtgcagt tcacaacggc ctctctcccg atcagcgcag
     2401 atgttgggga actaatttca acttttgtcg taatttatgt caaataactg acagtcgtaa
     2461 cttaagaccc taatcagcta atgttaatcc ttgacctgaa ttgcaggcat ctgaaacggc
     2521 tgtaatccag cttcgatctt aatgtcatag ttcaaattct cagtagattt aactgagcgt
     2581 ggtgcgtgtt tggccattgc catagttttt atatggactg aagtcaacga ccaaaagatc
     2641 tgctcaaggc agcccaactg cattagcaag attttggttg ctattcagtc aatatcagaa
     2701 ctacggcaat ggacgagtgc ggcgatgtca acctacatgt aactgagaat tcaaactatg
     2761 acattgagaa cgaagccaaa atacagcaaa tattgatgtg gttgaaactt gggtcaagga
     2821 tcaaatggac aatatttggc aaaaaagata aattttgagg tgaaattgtt tcctgacatt
     2881 gccatgttgg ttggtgtgaa actgcagcgc aagacaatga aggaagagac tttatagagt
     2941 aagctgcccc gaatttgaca tgttttgaac cattttttgt tcttaagtct tttcacataa
     3001 tgaaaattgc attaaaatga tcaatgttgg ttgaatatat ttagaattgc aatgttgaat
     3061 ttctccattt tgaatatttc ataaaaaatc tgcactgtac caccatgttt tgctcccgcc
     3121 acagatttct taattttaga gcattttatg gctacctcat ttcttaaatc tgagcaaagc
     3181 taccgcatgt tattgaaata tggcacagat tcgcattttt aaaggggatt ttggtgttca
     3241 agtcgtgact tgcatgtggt gggtgataat gcctgtgtat gtcaatgcat gtggttcgtg
     3301 ttgtgtggat tgactgagct gacttgtgga ataagtttgc catgttgaat tccaggcatg
     3361 ctactctgaa taaaggtctt tagaaaaaca gattccaata atgttttcat ttttttgtca
     3421 attttctaca ggtcagtgcg atcgcatcga taggctaggt ggaggctcag tgatgataag
     3481 tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc
     3541 gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt
     3601 tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag
     3661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4321 ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg
     4381 tcctcgtgat accaattcgg agcctgcttt tttgtacaaa cttgttgata atggcaattc
     4441 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4501 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4561 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4621 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc
     4681 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4741 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4801 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4861 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4921 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4981 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5041 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5101 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5161 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5221 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5281 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5341 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5401 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5461 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cagatacctg
     5521 aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc
     5581 agggttttcc cagtcacgac gttgtaaaac gacggccagt catgcataat ccgcacgcat
     5641 ctggaataag gaagtgccat tccgcctgac ct
//
