LOCUS       sequence-433886-        9902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Anillin + reconstitution of
            Anillin delta Anillin homology domain .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Budnar S, Husain KB, Gomez GA, Naghibosadat M, Varma A, Verma S, 
            Hamilton NA, Morris RG, Yap AS
  TITLE     Anillin Promotes Cell Contractility by Cyclic Resetting of RhoA 
            Residence Kinetics
  JOURNAL   Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 
            10.1016/j.devcel.2019.04.031. Epub 2019 May 16.
  PUBMED    31105010
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2019.04.031"; journalName: "Dev Cell"; date: 
            "2019-06-17- 17"; volume: "49"; issue: "6"; pages: "894-906.e12"
COMMENT     Sequence Label: PLL5.0-Anillin ShRNA-Cherry-Anillin deltaAnill...
FEATURES             Location/Qualifiers
     source          1..9902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    90..676
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(143..163)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(561..572)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(679..695)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             1205..1385
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(1447..2035)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1527..1546)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2206..2997,2998..3066))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2829..2848
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3067..3171)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3246..3265)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3437..3816
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3818..4016
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4034..4214
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4261..4386
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4883..5116
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5301..5345
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5643..5760
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5815..6127
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     6046..6066
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     6190..6206
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6248..6281)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        6361..6664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6665..6868
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      6897..6906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6903..7610
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7048..7066)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7316..7335
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatacatgt ttaagggttc cggttccact
       61 aggtacaatt cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa
      121 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
      181 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
      241 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
      301 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
      361 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
      421 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
      481 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
      541 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cggcctgctg
      601 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
      661 tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc
      721 aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga
      781 ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa
      841 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca
      901 ctcccaacga agacaagata tccttgatct gtggatctac cacacacaag gctacttccc
      961 tgattggcag aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg
     1021 ctacaagcta gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac
     1081 ccgcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga
     1141 gtggaggttt gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg
     1201 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     1261 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     1321 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1381 tagcagcatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1681 tcgctccaag ctgggctgtg tgctcgaacc ccccgttcag cccgaccgct gcgccttatc
     1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1861 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2521 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3181 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     3241 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     3301 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     3361 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     3421 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     3481 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     3541 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     3601 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     3661 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     3721 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     3781 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     3841 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     3901 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     3961 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     4021 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4081 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4141 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4201 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     4261 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     4321 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     4381 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     4441 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     4501 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     4561 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     4621 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     4681 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct
     4741 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     4801 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     4861 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     4921 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     4981 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     5041 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     5101 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     5161 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     5221 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     5281 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     5341 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     5401 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     5461 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     5521 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     5581 tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac
     5641 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     5701 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     5761 cgggtttatt acagggacag cagagatcca gtttggttag taccgcgctc tagagatccg
     5821 acgcgccatc tctaggcccg cgccggcccc ctcgcacaga cttgtgggag aagctcggct
     5881 actcccctgc cccggttaat ttgcatataa tatttcctag taactataga ggcttaatgt
     5941 gcgataaaag acagataatc tgttcttttt aatactagct acattttaca tgataggctt
     6001 ggatttctat aagagataca aatactaaat tattatttta aaaaacagca caaaaggaaa
     6061 ctcaccctaa ctgtaaagta attgtgtgtt ttgagactat aaatatccct tggagaaaag
     6121 ccttgtttcg gcgatgcctc tttgaataaa ttcaagagat ttattcaaag aggcatcgcc
     6181 attttttctc gaggtcgacg gtatcgataa gctcgcttca cgagattcca gcaggtcgag
     6241 ggacctaata acttcgtata gcatacatta tacgaagtta tattaagggt tccaagctta
     6301 agcggccgca tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     6361 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     6421 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     6481 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     6541 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     6601 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     6661 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     6721 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     6781 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     6841 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
     6901 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     6961 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     7021 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     7081 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     7141 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     7201 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     7261 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     7321 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     7381 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     7441 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     7501 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     7561 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ccgcggatgg
     7621 atccgtttac ggagaaactg ctggagcgaa cccgtgccag gcgagagaat cttcagagaa
     7681 aaatggctga gaggcccaca gcagctccaa ggtctatgac tcatgctaag cgagctagac
     7741 agccactttc agaagcaagt aaccagcagc ccctctctgg tggtgaagag aaatcttgta
     7801 caaaaccatc gccatcaaaa aaacgctgtt ctgacaacac tgaagtagaa gtttctaact
     7861 tggaaaataa acaaccagtt gagtcgacat ctgcaaaatc ttgttctcca agtcctgtgt
     7921 ctcctcaggt gcagccacaa gcagcagata ccatcagtga ttctgttgct gtcccggcat
     7981 cactgctggg catgaggaga gggctgaact caagattgga agcaactgca gcctcctcag
     8041 ttaaaacacg tatgcaaaaa cttgcagagc aacggcgccg ttgggataat gatgatatga
     8101 cagatgacat tcctgaaagc tcactcttct caccaatgcc atcagaggaa aaggctgctt
     8161 cccctcccag acctctgctt tcaaatgcct cggcaactcc agttggcaga aggggccgtc
     8221 tggccaatct tgctgcaact atttgctcct gggaagatga tgtaaatcac tcatttgcaa
     8281 aacaaaacag tgtacaagaa cagcctggta ccgcttgttt atccaaattt tcctctgcaa
     8341 gtggagcatc tgctaggatc aatagcagca gtgttaagca ggaagctaca ttctgttccc
     8401 aaagggatgg cgacgcaagc ttaaacaagg ccctatcctc aagtgctgct gatgcgtctt
     8461 tggttaatgc ctcaatttcc agctctgtga aagctacttc tccagtgaaa tctactacat
     8521 ctatcactga tgctaaaagt tgtgagggac aaaatcctga gctacttcca aaaactccta
     8581 ttagtcctct gaaaacgggg gtatcgaaac caattgtgaa gtcaacttta tcccagacag
     8641 ttccatccaa gggagaatta agtagagaaa tttgtctgca atctcaatct aaagacaaat
     8701 ctacgacacc aggaggaaca ggaattaagc ctttcctgga acgctttgga gagcgttgtc
     8761 aagaacatag caaagaaagt ccagctcgta gcacacccca cagaaccccc attattactc
     8821 caaatacaaa ggccatccaa gaaaaattat tcaagcaaga cacatcttca tctactaccc
     8881 atttagcaca acagctcaag caggaacgtc aaaaagaact agcatgtctt cgtggccgat
     8941 ttgacaaggg caatatatgg agtgcagaaa aaggcggaaa ctcaaaaagc aaacaactag
     9001 aaaccaaaca ggaaactcac tgtcagagca ctcccctcaa aaaacaccaa ggtgtttcaa
     9061 aaactcagtc acttccagta acagaaaagg tgaccgaaaa ccagatacca gccaaaaatt
     9121 ctagtacaga acctaaagaa gtgatacgtg aaattgagat gagtgtggat gatgatgata
     9181 tcaatagttc gaaagtaatt aatgacctct tcagtgatgt cctagaggaa ggtgaactag
     9241 atatggagaa gagccaagag gagatggatc aagcattagc agaaagcagc gaagaacagg
     9301 aagatgcact gaatatctcc tcaatgtctt tacttgcacc attggcacaa acagttggtg
     9361 tggtaagtcc agagagttta gtgtccacac ctagactgga attgaaagac accagcagaa
     9421 gtgatgaaag tccaaaacca aattccagtg ttgaagaaag aggttttcta accatatttg
     9481 aagatgttag tggttttggt gcctggcatc gaagatggtg tgttctttct ggaaactgta
     9541 tatcttattg gacttatcca gatgatgaga aacgcaagaa tcccatagga aggataaatc
     9601 tggctaattg taccagtcgt cagatagaac cagccaacag agaattttgt gcaagacgca
     9661 acacttttga attaattact gtccgaccac aaagagaaga tgaccgagag actcttgtca
     9721 gccaatgcag ggacacactc tgtgttacca agaactggct gtctgcagat actaaagaag
     9781 agcgggatct ctggatgcaa aaactcaatc aagttcttgt tgatattcgc ctctggcaac
     9841 ctgatgcttg ctacaaacct attggaaagc cttaacctgc aggcaattcg tcgagggacc
     9901 ta
//
