LOCUS       sequence-433887-        8838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of shRNA targeting Cavin1 + reconstitution of 
            mouse Cavin1 (Cavin1 rescue).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8838)
  AUTHORS   Teo JL, Gomez GA, Weeratunga S, Davies EM, Noordstra I, Budnar S, 
            Katsuno-Kambe H, McGrath MJ, Verma S, Tomatis V, Acharya BR, 
            Balasubramaniam L, Templin RM, McMahon KA, Lee YS, Ju RJ, Stebhens 
            SJ, Ladoux B, Mitchell CA, Collins BM, Parton RG, Yap AS
  TITLE     Caveolae Control Contractile Tension for Epithelia to Eliminate 
            Tumor Cells.
  JOURNAL   Dev Cell. 2020 Jul 6;54(1):75-91.e7. doi: 
            10.1016/j.devcel.2020.05.002. Epub 2020 Jun 1.
  PUBMED    32485139
REFERENCE   2  (bases 1 to 8838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2020.05.002"; journalName: "Dev Cell"; date: 
            "2020-07-6- 6"; volume: "54"; issue: "1"; pages: "75-91.e7"
COMMENT     Sequence Label: pLL5.0 shCavin1KDR-EGFP
FEATURES             Location/Qualifiers
     source          1..8838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        612..810
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             828..1008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1055..1180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2437..2554
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2609..2921
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2840..2860
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2978..2994
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3036..3069)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3657..3666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3663..3665,3666..3668,3669..4379)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3663..3665,3666..3668,3669..4379)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3708..3729)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3969..3988)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4316..4337
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5633..5666)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    5722..6308
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5775..5795)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6193..6204)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6311..6327)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             6837..7017
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7079..7667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7159..7178)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7838..8629,8630..8698))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8461..8480
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8699..8803)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      841 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      901 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      961 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     1021 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1081 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1141 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1201 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1261 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1321 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1381 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1441 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1501 gaccaccgca cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
     2401 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2461 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2521 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2581 tccagtttgg ttagtaccgc gctctagaga tccgacgcgc catctctagg cccgcgccgg
     2641 ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     2701 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     2761 ttttaatact agctacattt tacatgatag gcttggattt ctataagaga tacaaatact
     2821 aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     2881 tgttttgaga ctataaatat cccttggaga aaagccttgt tttgagcagc cggaccaata
     2941 attcaagaga ttattggtcc ggctgctcat tttttctcga ggtcgacggt atcgataagc
     3001 tcgcttcacg agattccagc aggtcgaggg acctaataac ttcgtatagc atacattata
     3061 cgaagttata ttaagggttc caagcttaag cggccgctga aagaccccac ctgtaggttt
     3121 ggcaagctag cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag
     3181 agaagttcag atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc
     3241 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc
     3301 ccgccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     3361 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     3421 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc
     3481 gatagactgc gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg
     3541 aatcgtggac tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg
     3601 gggtctttca tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
     3661 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     3721 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     3781 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     3841 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     3901 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     3961 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4021 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4081 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4141 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4201 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4261 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4321 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4381 ccggactcag atctcgagct caagcttcga attctatgga ggatgtcacg ctccatatcg
     4441 ttgagcggcc gtattccgga tttcccgatg cttcctcaga gggcccggag cccacccaag
     4501 gggaggcgcg ggccacggag gagccgtcgg ggaccggctc cgacgagctg atcaagtcgg
     4561 accaggtgaa cggtgtgctg gtgctgagcc ttctggataa aatcatcggc gccgttgacc
     4621 agatccagct gacccaagcc cagctggagg agcgacaggc ggagatggag ggcgctgtgc
     4681 agagcatcca gggagagctg agcaagctgg gcaaggcgca cgccaccacg agcaacaccg
     4741 tgagcaagtt gctggagaag gtgcgcaagg tcagcgtcaa cgtgaagacc gtgcgcggca
     4801 gcctggagcg ccaggccggc cagataaaga aactggaggt caacgaggcg gagctgctga
     4861 ggcgccgcaa cttcaaagtc atgatctacc aggatgaagt caagctgccg gccaaactga
     4921 gcgtcagcaa gtcgctgaaa gagtcggagg cactgcctga gaaggagggt gacgagctgg
     4981 gcgagggcga gcggcccgag gatgacaccg cggcgatcga gctgtcgtcc gacgaggcgg
     5041 tggaggtgga ggaggtgatc gaggagtccc gcgccgagcg catcaagcgc agcggcctgc
     5101 ggcgcgtgga cgacttcaag aaggccttct ccaaggagaa gatggagaag accaaggtgc
     5161 gcacgcgtga gaacctggag aagacgcgcc tgaagaccaa ggagaacctg gagaagacac
     5221 ggcacacgct ggagaagcgc atgaacaagc tgggcacgcg cctggtgccc gtggagcgac
     5281 gagagaagct gaagacatcc cgggacaagc tgcgcaagtc cttcacgccc gaccatgtgg
     5341 tgtatgcgcg ctccaagacc gctgtctaca aggtgccgcc tttcaccttc cacgtcaaga
     5401 agatccgcga gggcgaggtg gaggtgctga aggccaccga gatggtggag gtgggtcccg
     5461 aggacgacga ggttggcgcg gagcgcggcg aggccactga cctgctgcgc gggagcagcc
     5521 ccgacgtgca cacgctgctg gagatcaccg aggagtcgga cgccgtcctg gtggacaaga
     5581 gcgacagcga ctgaagtaca agtaacctgc aggcaattcg tcgagggacc taataacttc
     5641 gtatagcata cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa
     5701 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     5761 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5821 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5881 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5941 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     6001 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6061 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     6121 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6181 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccggcctgc tgccggctct
     6241 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     6301 ctccccgcat cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa
     6361 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6421 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     6481 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6541 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     6601 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     6661 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     6721 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     6781 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     6841 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     6901 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     6961 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     7021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     7081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     7141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     7201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     7261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     7321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7801 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7861 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7921 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7981 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     8041 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     8101 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     8161 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     8221 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     8281 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     8341 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8401 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8461 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8521 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8581 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8641 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8701 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8761 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8821 tgccacctga cgtcgacg
//
