LOCUS       sequence-433910-        9818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-terminal mCherry tag. Gateway destination vector for lentivirus 
            production and stable transduction in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9818)
  AUTHORS   Agrotis A, Pengo N, Burden JJ, Ketteler R
  TITLE     Redundancy of human ATG4 protease isoforms in autophagy and 
            LC3/GABARAP processing revealed in cells.
  JOURNAL   Autophagy. 2019 Jan 21. doi: 10.1080/15548627.2019.1569925.
  PUBMED    30661429
REFERENCE   2  (bases 1 to 9818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2019 Jan 21. doi: 10.1080/15548627.2019.1569925."
COMMENT     Sequence Label: pLVpuro-CMV-N-mCherry
FEATURES             Location/Qualifiers
     source          1..9818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    899..920
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(935..940,941..958,959..965)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    973..989
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     978..1000
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     997..1013
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     997..1013
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1032..1052
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1034..1052
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1080..1306
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1307..1487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1534..1659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2152..2385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2570..2614
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2912..2932
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3221..3424
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3375..3395
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        3469..3487
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3524..3533
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3530..4237
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3675..3693)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3943..3962
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    4259..4383
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4408..4413,4414..4431,4432..4438)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4492..5151
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4558..4577)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5472..5777
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5656..5675
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5818..5942)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    5991..6108
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6157..6667
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6586..6604
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6689..7288
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6689..6708)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7185..7205
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7416..7649
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7721..7842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7758..7777)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7812..7831
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7882..8017
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(8038..8056)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8066..8083)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8066..8082)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8075..8097)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8224..8679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8311..8330)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8521..8542
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8705..8809
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8810..8878,8879..9670)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9028..9047)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      901 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      961 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     1021 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     1081 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     1141 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     1201 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     1261 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     1321 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     1381 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     1441 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     1501 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     1561 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1621 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1681 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1741 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     1801 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1861 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1921 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1981 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     2041 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     2101 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     2161 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     2221 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     2281 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     2341 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     2401 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     2461 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     2521 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     2581 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     2641 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     2701 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     2761 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     2821 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg
     2881 agactagcct cgagcggccg cccccttcac cgagggccta tttcccatga ttccttcata
     2941 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac
     3001 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt
     3061 tttaaaatta tgttttaaaa tggactatca tatgccaagt acgcccccta ttgacgtcaa
     3121 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     3181 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     3241 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     3301 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     3361 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     3421 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     3481 ctatagggag acccaagctg gctagcttaa ttaagactct agagccacca tggtgagcaa
     3541 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     3601 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     3661 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     3721 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     3781 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     3841 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     3901 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     3961 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     4021 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     4081 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     4141 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     4201 ccgccactcc accggcggca tggacgagct ctacaaaagc ggttccggac cggatcaaac
     4261 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     4321 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     4381 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     4441 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
     4501 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     4561 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     4621 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     4681 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     4741 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     4801 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     4861 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     4921 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     4981 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     5041 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     5101 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga
     5161 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctcgcga accggtgtat
     5221 acccgaagta tgtcaaaaag aggtatgcta tgaagcagcg tattacagtg acagttgaca
     5281 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac
     5341 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga
     5401 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag
     5461 gggctggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt
     5521 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg
     5581 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg
     5641 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg
     5701 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga
     5761 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat
     5821 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa
     5881 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg
     5941 gtgtgattcg aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga
     6001 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     6061 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     6121 agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc gccttttcca
     6181 aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag
     6241 cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt
     6301 cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg
     6361 ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac
     6421 cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc
     6481 caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga
     6541 ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg
     6601 caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc
     6661 tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     6721 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6781 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6841 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6901 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6961 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     7021 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     7081 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     7141 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     7201 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     7261 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     7321 aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca aggcagctgt
     7381 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7441 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7501 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7561 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7621 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7681 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7741 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7801 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7861 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7921 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7981 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     8041 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8101 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8161 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     8221 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8401 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     8581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     8641 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     8701 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     9241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     9301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9781 ttaacgtgag ttttcgttcc actgagcgtc agaccccg
//
