LOCUS       sequence-433928-        8839 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Rozenblatt-Rosen O, Deo RC, Padi M, Adelmant G, Calderwood MA, 
            Rolland T, Grace M, Dricot A, Askenazi M, Tavares M, Pevzner SJ, 
            Abderazzaq F, Byrdsong D, Carvunis AR, Chen AA, Cheng J, Correll M, 
            Duarte M, Fan C, Feltkamp MC, Ficarro SB, Franchi R, Garg BK, 
            Gulbahce N, Hao T, Holthaus AM, James R, Korkhin A, Litovchick L, 
            Mar JC, Pak TR, Rabello S, Rubio R, Shen Y, Singh S, Spangle JM, 
            Tasan M, Wanamaker S, Webber JT, Roecklein-Canfield J, Johannsen E, 
            Barabasi AL, Beroukhim R, Kieff E, Cusick ME, Hill DE, Munger K, 
            Marto JA, Quackenbush J, Roth FP, DeCaprio JA, Vidal M
  TITLE     Interpreting cancer genomes using systematic host network 
            perturbations by tumour virus proteins.
  JOURNAL   Nature. 2012 Jul 26;487(7408):491-5.
  PUBMED    22810586
REFERENCE   2  (bases 1 to 8839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2012-07-26"; volume: "487(7408)"; pages: "491-5"
COMMENT     Sequence Label: MSCV-N JCV mad1 ER
FEATURES             Location/Qualifiers
     source          1..8839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1418..1427
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1427..1450
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1463..1489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1505..1529
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3941..3965)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3978..4545
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3995..4547
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4139..4156)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4366..4385
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4559..5158
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4559..4578)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5055..5075
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5234..5748
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    5910..5926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5934..5940,5941..5958,5959..5964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5979..6000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6117..6134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6288..6876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6368..6387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7047..7838,7839..7907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7670..7689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7908..8012)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8080..8098
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8136..8158)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8258..8277
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8471..8493
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8621..8640
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatctgcc accatggact acaaggatga
     1441 cgatgacaag ctcgatggag gatacccata cgatgttcca gattacgctg ctcgaggtta
     1501 tcaaacaagt ttgtacaaaa aagcaggcat ggacaaagtg ctgaataggg aggaatccat
     1561 ggagcttatg gatttattag gccttgatag gtctgcatgg gggaacattc ctgtcatgag
     1621 aaaagcttat ctgaaaaaat gcaaagaact ccaccctgat aaaggtgggg acgaagacaa
     1681 gatgaagaga atgaattttt tatataaaaa aatggaacaa ggtgtaaaag ttgctcatca
     1741 gcctgatttt ggtacatgga atagttcaga ggttggttgt gattttcctc ctaattctga
     1801 taccctttat tgcaaggaat ggcctaactg tgccactaat ccttcagtgc attgcccctg
     1861 tttaatgtgc atgctaaaat taaggcatag aaacagaaaa tttttaagaa gcagcccact
     1921 tgtgtggata gattgctatt gctttgattg cttcagacaa tggtttgggt gtgacttaac
     1981 ccaagaagct cttcattgct gggagaaagt tcttggagac accccctaca gggatctaaa
     2041 gctttaaggt aaaccactat ttttcttttg cagggcattt tgttttttac aataaactta
     2101 attgtttttt tttaggtgcc aacctatgga acagatgaat gggaatcctg gtggaataca
     2161 tttaatgaga agtgggatga agacctgttt tgccatgaag aaatgtttgc cagtgatgat
     2221 gaaaacacag gatcccaaca ctctacccca cctaaaaaga aaaaaaaggt agaagaccct
     2281 aaagactttc ctgtagatct gcatgcattc ctcagtcaag ctgtgtttag taatagaact
     2341 gttgcttctt ttgctgtgta taccactaaa gaaaaagctc aaattttata taagaaactt
     2401 atggaaaaat attctgtaac ttttataagt agacatggtt ttgggggtca taatattttg
     2461 tttttcttaa caccacatag acatagagtg tcagcaatta ataactactg tcaaaaacta
     2521 tgtaccttta gttttttaat ttgtaaaggt gtgaataagg aatacttgtt ttatagtgcc
     2581 ctgtgtagac agccatatgc agtagtggaa gaaagtattc aggggggcct taaggagcat
     2641 gactttaacc cagaagaacc agaagaaact aagcaggttt catggaaatt agttacacag
     2701 tatgccttgg aaaccaagtg tgaggatgtt tttttgctta tgggcatgta cttagacttt
     2761 caggaaaacc cacagcaatg caaaaaatgt gaaaaaaagg atcagccaaa tcactttaac
     2821 catcatgaaa aacactatta taatgcccaa atttttgcag atagcaaaaa tcaaaaaagc
     2881 atttgccagc aggctgttga tactgtagca gccaaacaaa gggttgacag catccacatg
     2941 accagagaag aaatgttagt tgaaaggttt aatttcttgc ttgataaaat ggacttaatt
     3001 tttggggcac atggcaatgc tgttttagag caatatatgg ctggggtggc ctggattcat
     3061 tgcttgctgc ctcaaatgga cactgttatt tatgactttc taaaatgcat tgtattaaac
     3121 attccaaaaa aaaggtactg gctattcaag gggccaatag acagtggcaa aactacttta
     3181 gctgcagctt tacttgatct ctgtggggga aagtcattaa atgttaatat gccattagaa
     3241 agattaaact ttgaattagg agtgggtata gatcagttta tggttgtatt tgaggatgta
     3301 aaaggcactg gtgcagagtc aagggattta ccttcagggc atggcataag caaccttgat
     3361 tgcttaagag attacttaga tggaagtgta aaagttaatt tagagagaaa acaccaaaac
     3421 aaaagaacac aggtgtttcc acctggaatt gtaaccatga atgaatattc agtgcctaga
     3481 actttacagg ccagatttgt aaggcagata gattttagac caaaggccta cctgagaaaa
     3541 tcactaagct gctctgagta tttgctagaa aaaaggattt tgcaaagtgg tatgactttg
     3601 cttttgcttt taatctggtt taggccagtt gctgactttg cagctgccat tcatgagagg
     3661 attgtgcagt ggaaagaaag gctggattta gaaataagca tgtatacatt ttctactatg
     3721 aaagctaatg ttggtatggg gagacccatt cttgactttc ctagagagga agattctgaa
     3781 gcagaagact ctggacatgg atcaagcact gaatcacaat cacaatgctt ttcccaggtc
     3841 tcagaagcct ctggtgcaga cacacaggaa aactgcactt ttcacatctg taaaggcttt
     3901 caatgtttca aaaaaccaaa gacccctccc ccaaaataag acccagcttt cttgtacaaa
     3961 gtggttcgat aacgaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa
     4021 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     4081 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     4141 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     4201 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     4261 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     4321 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     4381 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     4441 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     4501 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacaaccat
     4561 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     4621 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     4681 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     4741 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     4801 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     4861 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     4921 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     4981 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5041 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5101 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatg
     5161 catcgatgtc gacctgcagc caagcttatc gataaaataa aagattttat ttagtctcca
     5221 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     5281 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga
     5341 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     5401 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga
     5461 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     5521 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     5581 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac
     5641 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     5701 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcatgg gtaacagttt
     5761 cttgaagttg gagaacaaca ttctgagggt aggagtcgaa tattaagtaa tcctgactca
     5821 attagccact gttttgaatc cacatactcc aatactcctg aaatagttca ttatggacag
     5881 cgcagaaaga gctggggaga attgtgaaat tgttatccgc tcacaattcc acacaacata
     5941 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6001 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6061 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6961 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7021 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7081 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7141 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7201 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7261 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7321 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7381 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7441 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7501 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7561 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7621 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7681 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7741 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7801 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7861 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7921 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7981 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8041 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8101 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8161 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8221 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     8281 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     8341 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8401 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8461 ccagggtttt cccagtcacg acgttgtaaa acgacggcgc aaggaatggt gcatgcaagg
     8521 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     8581 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     8641 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagaggc
     8701 gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt ttgactcaac
     8761 aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag attttattta
     8821 gtctccagaa aaagggggg
//
