LOCUS       sequence-433965-       11508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a UAS controlled and Flp dependent conditional trangene of 
            bicistronic myrGFP and KD Recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, 
            Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, Wohlschlegel JA, Aso 
            Y, Zipursky SL
  TITLE     Mapping of multiple neurotransmitter receptor subtypes and distinct 
            protein complexes to the connectome.
  JOURNAL   Neuron. 2024 Jan 13:S0896-6273(23)00973-X. doi: 
            10.1016/j.neuron.2023.12.014.
  PUBMED    38262414
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Jan 13:S0896-6273(23)00973-X. doi: 
            10.1016/j.neuron.2023.12.014."
COMMENT     Sequence Label: pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
FEATURES             Location/Qualifiers
     source          1..11508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     507..526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    693..762
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(987..1005)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1457..1528,1930..2203,2278..2932,2994..3309,3513..3644,
                     3715..4329)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    5359..5453
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5487..5581
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5612..5850
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5797..5818
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    complement(5860..5893)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          6338..6403
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6533..6553
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6825..6959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6977..7010)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             7285..7995
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             8026..8091
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     intron          9642..9707
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9837..9857
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10129..10263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10305..10323)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10390..10494
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10495..10563,10564..11355)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10713..10732)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
       61 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      661 cacatgttac cgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc
      721 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac
      781 gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc
      841 tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt
      901 gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacaagc
      961 cgaacatatg ggcgcgccta gtatgtatgt aagttaataa aacccatttt tgcggaaagt
     1021 agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt atctgatcag
     1081 ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga ttacctctca
     1141 ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat
     1201 atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacg
     1261 tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga agctccaagc
     1321 ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt
     1381 tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt
     1441 gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa
     1501 acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc
     1561 acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac
     1621 ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca gattattctg
     1681 gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa gacatctaaa
     1741 ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga tattttaagc
     1801 tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcggg
     1861 gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta
     1921 tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag
     1981 gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg ttcagggagc
     2041 ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc
     2101 aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac
     2161 gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt tctattcgca
     2221 gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga aactcagttt
     2281 gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt gccggaaaga
     2341 cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta tcgccatccg
     2401 ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc aggtgcgcct
     2461 atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac ctgattttcc
     2521 aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc cgcgtggatc
     2581 aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt gtgcccggca
     2641 gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc gaggctctaa
     2701 ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc tttaccgccc
     2761 acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc atcctgacca
     2821 ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg atggccgagg
     2881 gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc tagtgagttc
     2941 gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc cagcgtgggt
     3001 gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt ggccgttgtg
     3061 cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa ttttgccatt
     3121 agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga gaagccactg
     3181 gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc
     3241 ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt
     3301 cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa
     3361 caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag tttttttcaa
     3421 tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac tcgcgaatat
     3481 taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct
     3541 ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc atcttcctct
     3601 tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt catgtttaga
     3661 atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc
     3721 acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca
     3781 tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc actggtcttc
     3841 acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc
     3901 ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc
     3961 gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg
     4021 ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg
     4081 tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg
     4141 gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc
     4201 atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc
     4261 attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc
     4321 aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac cattattacc
     4381 atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa
     4441 aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac
     4501 tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg
     4561 tttatcgcta cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg
     4621 ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc
     4681 acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt
     4741 tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc aatagaaatt
     4801 agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata tatttccctc
     4861 acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt tgtgacctgt
     4921 tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg ttccttgtgt
     4981 agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc aaaaataaca
     5041 actataataa taagaataca tttaatttag aaaatgcttg gatttcactg gaactagggc
     5101 gcgcctccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     5161 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     5221 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     5281 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc agggccgcaa
     5341 gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc tccgagcgga
     5401 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagac
     5461 tctagcccta gggcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc
     5521 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
     5581 gcggagactc tagcgctagc gacgtcgagc gccggagtat aaatagaggc gcttcgtcta
     5641 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     5701 aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa
     5761 tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa
     5821 gtaattattg aatacaagaa gagaactctg aatagatctg aagttcctat actttctaga
     5881 gaataggaac ttctaacgta agctagctag accggtgtcg actaaagcca aatagaaaat
     5941 tattcagttc ctggcttaag tttttaaaag tgatattatt tatttggttg taaccaacca
     6001 aaagaatgta aataactaat acataattat gttagtttta agttagcaac aaattgattt
     6061 tagctatatt agctacttgg ttaataaata gaatatattt atttaaagat aattgcgttt
     6121 ttattgtcag ggagtgagtt tgcttaaaaa ctcgtttagg tttgtcctcc cgaaattatt
     6181 tatttaaatg cgatggagag ttggcgccga atcgaaaact ttacgcgctt aaaagcacga
     6241 gttggcatcc ctaacgcgta ggatctttgt gaaggaacct tacttctgtg gtgtgacata
     6301 attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt
     6361 ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac
     6421 tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga
     6481 aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa
     6541 gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca
     6601 tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc
     6661 tgcactgcta tacaagaaaa ttatggaaaa atatttgatg tatagtgcct tgactagaga
     6721 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     6781 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     6841 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6901 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     6961 ctagtgatct ggccgggaag ttcctatact ttctagagaa taggaacttc ctcgagaatc
     7021 aaaatgggca acaaatgctg cagcaagcga caggatcagg aactggcact ggcctatccc
     7081 actgggggct acaagaaatc cgactacacc tttggccaga cgcacatcaa cagcagcggc
     7141 ggcggcaaca tgggcggcgt tcttggccag aagcataaca acggtggctc gctggactcg
     7201 cgctacacgc ccgatcccaa tcatcggggt ccgttgaaaa tcggcggaaa gggcggcgtt
     7261 gacatcatca gaccacgcgg atcctccaaa ggtgaagaac tgtttaccgg agtagtcccg
     7321 atattggttg aactcgacgg cgatgtcaac ggtcataaat tcagtgtgtc cggcgagggt
     7381 gagggcgacg ccacatacgg taagctgacg ttgaagttca tatgcaccac gggcaagctg
     7441 cccgtgccat ggccgacgtt ggtcacgacg ctgacgtatg gtgtccagtg tttcagccgt
     7501 taccccgatc atatgaagca gcatgacttt ttcaagtcgg cgatgccgga gggatacgtt
     7561 caagagagga ccattttctt caaggatgac ggcaactata agacgcgagc ggaggtgaaa
     7621 tttgaaggcg acacactcgt taaccgtatt gagttgaagg gcattgattt taaggaggat
     7681 ggcaacattc tgggccataa gttggaatac aactacaact cgcataatgt gtatataatg
     7741 gcagataagc agaaaaacgg aataaaggtt aacttcaaga ttcgccacaa catagaggac
     7801 ggttccgtgc aacttgcaga tcattaccaa cagaacacac ccattggaga tggcccagtt
     7861 ctcttgccag acaatcacta cctttccaca cagtccgcgt tgagcaagga ccccaatgaa
     7921 aagcgggacc acatggtgtt gctggagttt gtgaccgcag ctggtattac acacggcatg
     7981 gacgagctgt acaagcgggc taagagatca ggttctggag caccagtgaa acagactttg
     8041 aattttgacc ttctcaagtt ggcaggagac gtggagtcca acccagggcc catgagtact
     8101 tttgcagaag cagcgcatct tacaccgcat caatgtgcga acgaaatcaa cgagattctg
     8161 gaaagtgata cattcaacat taatgcgaaa gaaatacgca acaagttggc tagtctcttt
     8221 tccattctta ccatgcaatc gctgtccatc cgtagggaaa tgaagattaa cacgtatcgt
     8281 agctacaagt ccgcaatcgg aaaatccctg agctttgaca aggatgacaa gattatcaaa
     8341 ttcactgtac ggctgaggaa gaccgagagt ctgcagaaag acattgaaag cgcactcccg
     8401 tcctacaaag tcgttgtttc gccattcaaa aaccaggagg tgtcgctttt cgatcgatac
     8461 gaggaaaccc acaagtacga tgcgtccatg gtgggcctcc aatttacgaa cattctcagc
     8521 aaggagaaag acatctggaa aatcgttagc cgaatcgctt gcttcttcga tcagtcgtgc
     8581 gtgacgacca cgaagcgagc ggagtaccgc ctgcttctgc tgggagccgt aggtaactgt
     8641 tgccgttata gcgatctcaa aaatctggac ccccggacct ttgagattta caacaattcc
     8701 ttcttgggac caatcgtgcg ggccacggtt acagagacaa agagtcgcac agagcgatat
     8761 gtgaactttt acccagtgaa tggtgattgc gatctgttga tttccctcta cgattacctc
     8821 cgagtttgct ccccgatcga gaagactgtg tcgagcaatc ggccgacaaa ccagacgcac
     8881 cagttcctcc cggaatccct tgcgcggacc ttcagccgct tcctgaccca gcacgttgac
     8941 gagccagtct tcaagatttg gaacggcccg aagtcgcact tcggtcggca cctgatggct
     9001 accttcttgt cgcgctccga aaagggaaag tatgtttcct ccctgggcaa ctgggcaggt
     9061 gaccgcgaaa tccagagcgc cgtcgcgcgc tcccactact cgcatggttc cgttaccgtt
     9121 gacgaccggg tctttgcatt catatcggga ttttacaaag aagccccgct gggttccgag
     9181 atttatgtgt tgaaggaccc ctccaacaag ccactcagca gggaggagct gcttgaggag
     9241 gagggcaact cgctcggaag tcccccactg agtcctccct cctcgccccg actggtggcc
     9301 cagtcgttca gtgcacaccc gtcgctgcaa ctcttcgaac agtggcacgg catcatcagc
     9361 gacgaggtcc tgcaatttat tgcggaatat aggcgcaagc acgagctgcg gagccagcgt
     9421 acggtggttg caggatccca tggcttccct ccagaggtgg aggagcaaga tgatggcact
     9481 ctccccatga gctgcgctca agagagtggc atggatcgtc accccgctgc ttgcgcctcg
     9541 gctcgcatca acgtgtaatc tagaggatct ttgtgaagga accttacttc tgtggtgtga
     9601 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     9661 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     9721 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     9781 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     9841 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     9901 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     9961 aagctgcact gctatacaag aaaattatgg aaaaatattt gatgtatagt gccttgacta
    10021 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
    10081 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
    10141 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    10201 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    10261 atcgatctgg ccggccgttt aaacgaattc ttgaagacga aagggcctcg tgatacgcct
    10321 atttttatag gttaatgtca tgataataat ggtttcttag actcaggtgg cacttttcgg
    10381 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
    10441 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
    10501 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
    10561 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
    10621 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
    10681 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
    10741 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
    10801 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
    10861 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
    10921 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
    10981 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
    11041 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
    11101 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
    11161 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
    11221 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
    11281 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    11341 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    11401 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    11461 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaa
//
