LOCUS       sequence-433973-       12225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a UAS controlled and Flp dependent conditional trangene of 
            bicistronic myrTdtomato and KD Recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, 
            Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, Wohlschlegel JA, Aso 
            Y, Zipursky SL
  TITLE     Mapping of multiple neurotransmitter receptor subtypes and distinct 
            protein complexes to the connectome.
  JOURNAL   Neuron. 2024 Jan 13:S0896-6273(23)00973-X. doi: 
            10.1016/j.neuron.2023.12.014.
  PUBMED    38262414
REFERENCE   2  (bases 1 to 12225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Jan 13:S0896-6273(23)00973-X. doi: 
            10.1016/j.neuron.2023.12.014."
COMMENT     Sequence Label: pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::...
FEATURES             Location/Qualifiers
     source          1..12225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      37..625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     526..545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    712..781
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(1006..1024)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1476..1547,1949..2222,2297..2951,3013..3328,3532..3663,
                     3734..4348)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    5378..5472
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5506..5600
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        5631..5869
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5816..5837
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    complement(5879..5912)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          6357..6422
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6552..6572
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6844..6978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6996..7029)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             7304..8731
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             8762..8827
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     intron          10378..10443
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10573..10593
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10865..10999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11041..11059)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11126..11230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11231..11299,11300..12091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11449..11468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
       61 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
      121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
      181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
      241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
      301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
      361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
      421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
      601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
      661 cttttgctgg ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc
      721 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
      781 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
      841 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
      901 tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
      961 ggcgggcatg tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa
     1021 acccattttt gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc
     1081 attgaactta tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg
     1141 ttctttcgat tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc
     1201 cttctctgtc cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc
     1261 caccgtttgt agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt
     1321 tcgtgacgaa gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac
     1381 tcctctcgct tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg
     1441 tgggtaagca ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat
     1501 taattcgcgg aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat
     1561 gtgcatacat cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct
     1621 gcggcgtgag aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga
     1681 cacgaaacag attattctgg tagctgttct cgctatataa gacaattttt gagatcatat
     1741 catgatcaag acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata
     1801 acaaccagat attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta
     1861 cttcgtagga tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt
     1921 tgatatttga gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat
     1981 taaccagggc ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga
     2041 ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat
     2101 ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac
     2161 acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa
     2221 cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta
     2281 ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc
     2341 agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc
     2401 atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag
     2461 atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc
     2521 agggaacacc tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag
     2581 cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg
     2641 atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca
     2701 ttcgcctccg aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga
     2761 ctggactcct ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc
     2821 aagaccgtca tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag
     2881 atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac
     2941 ttcttttcct agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca
     3001 actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt
     3061 acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat
     3121 atgcgacaat tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa
     3181 aaatctggag aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt
     3241 catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct
     3301 cgtaaaagtg cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa
     3361 cgcttacaat tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac
     3421 ccttcttagt ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt
     3481 tcatttaact cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc
     3541 atcttgattg gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc
     3601 aacggagcca tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata
     3661 aatgtaagtc atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat
     3721 cgattcgatt taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg
     3781 actttatcgc tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct
     3841 cacagtgcca ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt
     3901 gctgcacttc ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt
     3961 cggatatcta atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc
     4021 ggttatcata ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt
     4081 atacctcaaa tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat
     4141 taaccaatgg gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg
     4201 ccccagttcg ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct
     4261 ggactacgtg ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct
     4321 aagacttcgg gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt
     4381 tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta
     4441 attatatttg tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg
     4501 aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat
     4561 accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac
     4621 atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt
     4681 tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct
     4741 tggagcgact gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca
     4801 tttcgtctca atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat
     4861 atttgtatat atttccctca cagctatatt tattctaatt taatattatg actttttaag
     4921 gtaatttttt gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc
     4981 cagtgtttgt tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt
     5041 atatatatca aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg
     5101 atttcactgg aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg
     5161 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     5221 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     5281 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     5341 cccgtggcca gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga
     5401 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     5461 ctgtcctccg agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc
     5521 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
     5581 gcggagtact gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata
     5641 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     5701 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     5761 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     5821 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctga
     5881 agttcctata ctttctagag aataggaact tctaacgtaa gctagctaga ccggtgtcga
     5941 ctaaagccaa atagaaaatt attcagttcc tggcttaagt ttttaaaagt gatattattt
     6001 atttggttgt aaccaaccaa aagaatgtaa ataactaata cataattatg ttagttttaa
     6061 gttagcaaca aattgatttt agctatatta gctacttggt taataaatag aatatattta
     6121 tttaaagata attgcgtttt tattgtcagg gagtgagttt gcttaaaaac tcgtttaggt
     6181 ttgtcctccc gaaattattt atttaaatgc gatggagagt tggcgccgaa tcgaaaactt
     6241 tacgcgctta aaagcacgag ttggcatccc taacgcgtag gatctttgtg aaggaacctt
     6301 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
     6361 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
     6421 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
     6481 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
     6541 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
     6601 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
     6661 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tatttgatgt
     6721 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     6781 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     6841 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6901 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6961 tcttatcatg tctggatcac tagtgatctg gccgggaagt tcctatactt tctagagaat
     7021 aggaacttcc tcgagaatca aaatgggcaa caaatgctgc agcaagcgac aggatcagga
     7081 actggcactg gcctatccca ctgggggcta caagaaatcc gactacacct ttggccagac
     7141 gcacatcaac agcagcggcg gcggcaacat gggcggcgtt cttggccaga agcataacaa
     7201 cggtggctcg ctggactcgc gctacacgcc cgatcccaat catcggggtc cgttgaaaat
     7261 cggcggaaag ggcggcgttg acatcatcag accacgcgga tccatggtga gcaagggcga
     7321 ggaggtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
     7381 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     7441 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
     7501 catgtacggc tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct
     7561 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt
     7621 gaccgtgacc caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg
     7681 cggcaccaac ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     7741 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct
     7801 gaagctgaag gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa
     7861 gcccgtgcaa ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa
     7921 cgaggactac accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct
     7981 ggggcatggc accggcagca ccggcagcgg cagctccggc accgcctcct ccgaggacaa
     8041 caacatggcc gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa
     8101 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     8161 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     8221 ccagttcatg tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa
     8281 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     8341 tctggtgacc gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa
     8401 gatgcgcggc accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg
     8461 ggaggcctcc accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca
     8521 ggccctgaag ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc
     8581 caagaagccc gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc
     8641 ccacaacgag gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct
     8701 gttcctgtac ggcatggacg agctgtacaa gcgggctaag agatcaggtt ctggagcacc
     8761 agtgaaacag actttgaatt ttgaccttct caagttggca ggagacgtgg agtccaaccc
     8821 agggcccatg agtacttttg cagaagcagc gcatcttaca ccgcatcaat gtgcgaacga
     8881 aatcaacgag attctggaaa gtgatacatt caacattaat gcgaaagaaa tacgcaacaa
     8941 gttggctagt ctcttttcca ttcttaccat gcaatcgctg tccatccgta gggaaatgaa
     9001 gattaacacg tatcgtagct acaagtccgc aatcggaaaa tccctgagct ttgacaagga
     9061 tgacaagatt atcaaattca ctgtacggct gaggaagacc gagagtctgc agaaagacat
     9121 tgaaagcgca ctcccgtcct acaaagtcgt tgtttcgcca ttcaaaaacc aggaggtgtc
     9181 gcttttcgat cgatacgagg aaacccacaa gtacgatgcg tccatggtgg gcctccaatt
     9241 tacgaacatt ctcagcaagg agaaagacat ctggaaaatc gttagccgaa tcgcttgctt
     9301 cttcgatcag tcgtgcgtga cgaccacgaa gcgagcggag taccgcctgc ttctgctggg
     9361 agccgtaggt aactgttgcc gttatagcga tctcaaaaat ctggaccccc ggacctttga
     9421 gatttacaac aattccttct tgggaccaat cgtgcgggcc acggttacag agacaaagag
     9481 tcgcacagag cgatatgtga acttttaccc agtgaatggt gattgcgatc tgttgatttc
     9541 cctctacgat tacctccgag tttgctcccc gatcgagaag actgtgtcga gcaatcggcc
     9601 gacaaaccag acgcaccagt tcctcccgga atcccttgcg cggaccttca gccgcttcct
     9661 gacccagcac gttgacgagc cagtcttcaa gatttggaac ggcccgaagt cgcacttcgg
     9721 tcggcacctg atggctacct tcttgtcgcg ctccgaaaag ggaaagtatg tttcctccct
     9781 gggcaactgg gcaggtgacc gcgaaatcca gagcgccgtc gcgcgctccc actactcgca
     9841 tggttccgtt accgttgacg accgggtctt tgcattcata tcgggatttt acaaagaagc
     9901 cccgctgggt tccgagattt atgtgttgaa ggacccctcc aacaagccac tcagcaggga
     9961 ggagctgctt gaggaggagg gcaactcgct cggaagtccc ccactgagtc ctccctcctc
    10021 gccccgactg gtggcccagt cgttcagtgc acacccgtcg ctgcaactct tcgaacagtg
    10081 gcacggcatc atcagcgacg aggtcctgca atttattgag gaatataggc gcaagcacga
    10141 gctgcggagc cagcgtacgg tggttgcagg atcccatggc ttccctccag aggtggagga
    10201 gcaagatgat ggcactctcc ccatgagctg cgctcaagag agtggcatgg atcgtcaccc
    10261 cgctgcttgc gcctcggctc gcatcaacgt gtaatctaga ggatctttgt gaaggaacct
    10321 tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta
    10381 aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt
    10441 tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa
    10501 aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa
    10561 cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa
    10621 ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt
    10681 tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atatttgatg
    10741 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
    10801 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    10861 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10921 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    10981 atcttatcat gtctggatcg atctggccgg ccgtttaaac gaattcttga agacgaaagg
    11041 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagactc
    11101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
    11161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
    11221 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
    11281 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
    11341 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
    11401 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
    11461 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
    11521 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
    11581 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
    11641 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
    11701 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
    11761 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
    11821 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
    11881 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
    11941 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    12001 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    12061 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    12121 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    12181 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactg
//
