LOCUS       sequence-433987-        9578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses inactivated S. pyogenes dCas9 (D10A, H840A) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Perez-Pinera P, Kocak DD, Vockley CM, Adler AF, Kabadi AM, Polstein 
            LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford
            GE, Reddy TE, Gersbach CA
  TITLE     RNA-guided gene activation by CRISPR-Cas9-based transcription 
            factors.
  JOURNAL   Nat Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600.
  PUBMED    23892895
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Jul 25. doi: 10.1038/nmeth.2600."
COMMENT     Sequence Label: pcDNA-dCas9
FEATURES             Location/Qualifiers
     source          1..9578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(60..80)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        65..394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      245..380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     307..326
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             461..1255
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(515..534)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1125..1144
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1429..1550
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1466..1485)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1520..1539
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1599..1615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1599..1615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1612..1634)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1623..1639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1647..1653,1654..1671,1672..1677))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1692..1713
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1830..1847)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2001..2589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2081..2100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2760..3551,3552..3620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3383..3402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3621..3725)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3800..3819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3991..4370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4371..4574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4525..4545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4619..4638
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4619..4637
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4663..4728
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4735..4755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4765..4791,4792..4794,4795..7281,7282..7284,
                     7285..8868)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8869..8889
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(8925..8942)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8931..9155
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9201..9578,1..51)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9288..9307)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9498..9519
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
       61 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      121 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      301 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
      361 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
      421 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
      481 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
      541 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
      601 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
      661 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      721 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      781 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      841 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      901 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      961 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     1021 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     1081 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     1141 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     1201 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     1261 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     1321 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     1381 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     1441 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1501 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     1561 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     1621 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     1681 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     1741 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     1801 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1861 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1921 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1981 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2041 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2101 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2161 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2221 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2281 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2341 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2401 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2461 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2521 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2581 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2641 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2701 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2761 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2821 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2881 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2941 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3001 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3061 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3121 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3181 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3241 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3301 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3361 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3421 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3481 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3541 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3601 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3661 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3721 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     3781 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     3841 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     3901 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     3961 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     4021 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     4081 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     4141 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4201 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     4261 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     4321 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     4381 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4441 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     4501 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     4561 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
     4621 atacgactca ctatagggag acccaagctg gctagcgcca tggactacaa agaccatgac
     4681 ggtgattata aagatcatga catcgattac aaggatgacg atgacaagat ggcccccaag
     4741 aagaagagga aggtgggccg cggaatggat aagaaatact caataggctt agctatcggc
     4801 acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc taaaaagttc
     4861 aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg ggctctttta
     4921 tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg tagaaggtat
     4981 acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga gatggcgaaa
     5041 gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga agacaagaag
     5101 catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca tgagaaatat
     5161 ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc ggatttgcgc
     5221 ttaatctatt tggccttagc gcatatgatt aagtttcgtg gtcatttttt gattgaggga
     5281 gatttaaatc ctgataatag tgatgtggac aaactattta tccagttggt acaaacctac
     5341 aatcaattat ttgaagaaaa ccctattaac gcaagtggag tagatgctaa agcgattctt
     5401 tctgcacgat tgagtaaatc aagacgatta gaaaatctca ttgctcagct ccccggtgag
     5461 aagaaaaatg gcttatttgg gaatctcatt gctttgtcat tgggtttgac ccctaatttt
     5521 aaatcaaatt ttgatttggc agaagatgct aaattacagc tttcaaaaga tacttacgat
     5581 gatgatttag ataatttatt ggcgcaaatt ggagatcaat atgctgattt gtttttggca
     5641 gctaagaatt tatcagatgc tattttactt tcagatatcc taagagtaaa tactgaaata
     5701 actaaggctc ccctatcagc ttcaatgatt aaacgctacg atgaacatca tcaagacttg
     5761 actcttttaa aagctttagt tcgacaacaa cttccagaaa agtataaaga aatctttttt
     5821 gatcaatcaa aaaacggata tgcaggttat attgatgggg gagctagcca agaagaattt
     5881 tataaattta tcaaaccaat tttagaaaaa atggatggta ctgaggaatt attggtgaaa
     5941 ctaaatcgtg aagatttgct gcgcaagcaa cggacctttg acaacggctc tattccccat
     6001 caaattcact tgggtgagct gcatgctatt ttgagaagac aagaagactt ttatccattt
     6061 ttaaaagaca atcgtgagaa gattgaaaaa atcttgactt ttcgaattcc ttattatgtt
     6121 ggtccattgg cgcgtggcaa tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca
     6181 attaccccat ggaattttga agaagttgtc gataaaggtg cttcagctca atcatttatt
     6241 gaacgcatga caaactttga taaaaatctt ccaaatgaaa aagtactacc aaaacatagt
     6301 ttgctttatg agtattttac ggtttataac gaattgacaa aggtcaaata tgttactgaa
     6361 ggaatgcgaa aaccagcatt tctttcaggt gaacagaaga aagccattgt tgatttactc
     6421 ttcaaaacaa atcgaaaagt aaccgttaag caattaaaag aagattattt caaaaaaata
     6481 gaatgttttg atagtgttga aatttcagga gttgaagata gatttaatgc ttcattaggt
     6541 acctaccatg atttgctaaa aattattaaa gataaagatt ttttggataa tgaagaaaat
     6601 gaagatatct tagaggatat tgttttaaca ttgaccttat ttgaagatag ggagatgatt
     6661 gaggaaagac ttaaaacata tgctcacctc tttgatgata aggtgatgaa acagcttaaa
     6721 cgtcgccgtt atactggttg gggacgtttg tctcgaaaat tgattaatgg tattagggat
     6781 aagcaatctg gcaaaacaat attagatttt ttgaaatcag atggttttgc caatcgcaat
     6841 tttatgcagc tgatccatga tgatagtttg acatttaaag aagacattca aaaagcacaa
     6901 gtgtctggac aaggcgatag tttacatgaa catattgcaa atttagctgg tagccctgct
     6961 attaaaaaag gtattttaca gactgtaaaa gttgttgatg aattggtcaa agtaatgggg
     7021 cggcataagc cagaaaatat cgttattgaa atggcacgtg aaaatcagac aactcaaaag
     7081 ggccagaaaa attcgcgaga gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga
     7141 agtcagattc ttaaagagca tcctgttgaa aatactcaat tgcaaaatga aaagctctat
     7201 ctctattatc tccaaaatgg aagagacatg tatgtggacc aagaattaga tattaatcgt
     7261 ttaagtgatt atgatgtcga tgccattgtt ccacaaagtt tccttaaaga cgattcaata
     7321 gacaataagg tcttaacgcg ttctgataaa aatcgtggta aatcggataa cgttccaagt
     7381 gaagaagtag tcaaaaagat gaaaaactat tggagacaac ttctaaacgc caagttaatc
     7441 actcaacgta agtttgataa tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat
     7501 aaagctggtt ttatcaaacg ccaattggtt gaaactcgcc aaatcactaa gcatgtggca
     7561 caaattttgg atagtcgcat gaatactaaa tacgatgaaa atgataaact tattcgagag
     7621 gttaaagtga ttaccttaaa atctaaatta gtttctgact tccgaaaaga tttccaattc
     7681 tataaagtac gtgagattaa caattaccat catgcccatg atgcgtatct aaatgccgtc
     7741 gttggaactg ctttgattaa gaaatatcca aaacttgaat cggagtttgt ctatggtgat
     7801 tataaagttt atgatgttcg taaaatgatt gctaagtctg agcaagaaat aggcaaagca
     7861 accgcaaaat atttctttta ctctaatatc atgaacttct tcaaaacaga aattacactt
     7921 gcaaatggag agattcgcaa acgccctcta atcgaaacta atggggaaac tggagaaatt
     7981 gtctgggata aagggcgaga ttttgccaca gtgcgcaaag tattgtccat gccccaagtc
     8041 aatattgtca agaaaacaga agtacagaca ggcggattct ccaaggagtc aattttacca
     8101 aaaagaaatt cggacaagct tattgctcgt aaaaaagact gggatccaaa aaaatatggt
     8161 ggttttgata gtccaacggt agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg
     8221 aaatcgaaga agttaaaatc cgttaaagag ttactaggga tcacaattat ggaaagaagt
     8281 tcctttgaaa aaaatccgat tgacttttta gaagctaaag gatataagga agttaaaaaa
     8341 gacttaatca ttaaactacc taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg
     8401 atgctggcta gtgccggaga attacaaaaa ggaaatgagc tggctctgcc aagcaaatat
     8461 gtgaattttt tatatttagc tagtcattat gaaaagttga agggtagtcc agaagataac
     8521 gaacaaaaac aattgtttgt ggagcagcat aagcattatt tagatgagat tattgagcaa
     8581 atcagtgaat tttctaagcg tgttatttta gcagatgcca atttagataa agttcttagt
     8641 gcatataaca aacatagaga caaaccaata cgtgaacaag cagaaaatat tattcattta
     8701 tttacgttga cgaatcttgg agctcccgct gcttttaaat attttgatac aacaattgat
     8761 cgtaaacgat atacgtctac aaaagaagtt ttagatgcca ctcttatcca tcaatccatc
     8821 actggtcttt atgaaacacg cattgatttg agtcagctag gaggtgaccc caagaagaag
     8881 aggaaggtgg gctgatctag acttaagttt aaaccgctga tcagcctcga ctgtgccttc
     8941 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     9001 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     9061 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     9121 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     9181 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     9241 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     9301 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     9361 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     9421 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     9481 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     9541 ggtctattct tttgatttat aagggatttt gccgattt
//
