LOCUS       sequence-433988-       11637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a LexAoP controlled and Flp dependent conditional trangene 
            of bicistronic myrGFP and KD Recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Sanfilippo P, Kim AJ, Bhukel A, Yoo J, Mirshahidi PS, Pandey V, 
            Bevir H, Yuen A, Mirshahidi PS, Guo P, Li HS, Wohlschlegel JA, Aso 
            Y, Zipursky SL
  TITLE     Mapping of multiple neurotransmitter receptor subtypes and distinct 
            protein complexes to the connectome.
  JOURNAL   Neuron. 2024 Jan 13:S0896-6273(23)00973-X. doi: 
            10.1016/j.neuron.2023.12.014.
  PUBMED    38262414
REFERENCE   2  (bases 1 to 11637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2024 Jan 13:S0896-6273(23)00973-X. doi: 
            10.1016/j.neuron.2023.12.014."
COMMENT     Sequence Label: pJFRC-13XLexAoP-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
FEATURES             Location/Qualifiers
     source          1..11637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      18..606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     507..526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    693..762
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(987..1005)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1457..1528,1930..2203,2278..2932,2994..3309,3513..3644,
                     3715..4329)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     promoter        5741..5979
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     5926..5947
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    complement(5989..6022)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          6467..6532
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6662..6682
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6954..7088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7106..7139)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             7414..8124
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             8155..8220
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     intron          9771..9836
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9966..9986
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10258..10392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10434..10452)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10519..10623
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10624..10692,10693..11484)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10842..10861)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
       61 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      121 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      181 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      241 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      301 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
      361 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
      421 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      481 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      541 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      601 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      661 cacatgttac cgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc
      721 gtgcccttgg gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac
      781 gggtcgaggt ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc
      841 tcgaaaccgc tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt
      901 gcggatacgc ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacaagc
      961 cgaacatatg ggcgcgccta gtatgtatgt aagttaataa aacccatttt tgcggaaagt
     1021 agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt atctgatcag
     1081 ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga ttacctctca
     1141 ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat
     1201 atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacg
     1261 tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga agctccaagc
     1321 ggtttacgcc atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt
     1381 tgtttgtttt ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt
     1441 gtgaaaaatc ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa
     1501 acacccatct gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc
     1561 acttgatcta taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac
     1621 ccacaaaaat cccaaaccgc aattgcacaa acaaatagtg acacgaaaca gattattctg
     1681 gtagctgttc tcgctatata agacaatttt tgagatcata tcatgatcaa gacatctaaa
     1741 ggcattcatt ttcgactata ttctttttta caaaaaatat aacaaccaga tattttaagc
     1801 tgatcctaga tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcggg
     1861 gtactttttg ttcggggtta gatgagcata acgcttgtag ttgatatttg agatccccta
     1921 tcattgcagg gtgacagcgg agcggcttcg cagagctgca ttaaccaggg cttcgggcag
     1981 gccaaaaact acggcacgct ccggccaccc agtccgccgg aggactccgg ttcagggagc
     2041 ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt tggggcggtc
     2101 aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact attctgcaac
     2161 gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt tctattcgca
     2221 gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga aactcagttt
     2281 gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt gccggaaaga
     2341 cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta tcgccatccg
     2401 ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc aggtgcgcct
     2461 atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac ctgattttcc
     2521 aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc cgcgtggatc
     2581 aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt gtgcccggca
     2641 gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc gaggctctaa
     2701 ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc tttaccgccc
     2761 acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc atcctgacca
     2821 ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg atggccgagg
     2881 gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc tagtgagttc
     2941 gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc cagcgtgggt
     3001 gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt ggccgttgtg
     3061 cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa ttttgccatt
     3121 agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga gaagccactg
     3181 gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt ccgggcggtc
     3241 ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt
     3301 cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa ttcttggaaa
     3361 caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag tttttttcaa
     3421 tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac tcgcgaatat
     3481 taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct
     3541 ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc atcttcctct
     3601 tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt catgtttaga
     3661 atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc
     3721 acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca
     3781 tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc actggtcttc
     3841 acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc
     3901 ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc
     3961 gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg
     4021 ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg
     4081 tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg
     4141 gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc
     4201 atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc
     4261 attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc
     4321 aaggagtagc cgacatatat ccgaaataac tgcttgtttt ttttttttac cattattacc
     4381 atcgtgttta ctgtttattg ccccctcaaa aagctaatgt aattatattt gtgccaataa
     4441 aaacaagata tgacctatag aatacaagta tttccccttc gaacatcccc acaagtagac
     4501 tttggatttg tcttctaacc aaaagactta cacacctgca taccttacat caaaaactcg
     4561 tttatcgcta cataaaacac cgggatatat tttttatata catacttttc aaatcgcgcg
     4621 ccctcttcat aattcacctc caccacacca cgtttcgtag ttgctctttc gctgtctccc
     4681 acccgctctc cgcaacacat tcaccttttg ttcgacgacc ttggagcgac tgtcgttagt
     4741 tccgcgcgat tcggttcgct caaatggttc cgagtggttc atttcgtctc aatagaaatt
     4801 agtaataaat atttgtatgt acaatttatt tgctccaata tatttgtata tatttccctc
     4861 acagctatat ttattctaat ttaatattat gactttttaa ggtaattttt tgtgacctgt
     4921 tcggagtgat tagcgttaca atttgaactg aaagtgacat ccagtgtttg ttccttgtgt
     4981 agatgcatct caaaaaaatg gtgggcataa tagtgttgtt tatatatatc aaaaataaca
     5041 actataataa taagaataca tttaatttag aaaatgcttg gatttcactg gaactagggc
     5101 gcgcctccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac
     5161 acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc
     5221 gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca
     5281 gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc agggccgcaa
     5341 gcttgcatgc ctgcaggtta ctgtacatcc atacagtaag tactgtacat ccatacagta
     5401 agtactgtac atccatacag taagtactgt acatccatac agtaagtact gtacatccat
     5461 acagtaagcg gagactctag ccctagggca tgcctgcagg ttactgtaca tccatacagt
     5521 aagtactgta catccataca gtaagtactg tacatccata cagtaagcgg agactctagc
     5581 gctagcgcat gcctgcaggt tactgtacat ccatacagta agtactgtac atccatacag
     5641 taagtactgt acatccatac agtaagtact gtacatccat acagtaagta ctgtacatcc
     5701 atacagtaag cggagactct agcactagtg acgtcgagcg ccggagtata aatagaggcg
     5761 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     5821 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
     5881 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
     5941 tgccaagaag taattattga atacaagaag agaactctga atagatctga agttcctata
     6001 ctttctagag aataggaact tctaacgtaa gctagctaga ccggtgtcga ctaaagccaa
     6061 atagaaaatt attcagttcc tggcttaagt ttttaaaagt gatattattt atttggttgt
     6121 aaccaaccaa aagaatgtaa ataactaata cataattatg ttagttttaa gttagcaaca
     6181 aattgatttt agctatatta gctacttggt taataaatag aatatattta tttaaagata
     6241 attgcgtttt tattgtcagg gagtgagttt gcttaaaaac tcgtttaggt ttgtcctccc
     6301 gaaattattt atttaaatgc gatggagagt tggcgccgaa tcgaaaactt tacgcgctta
     6361 aaagcacgag ttggcatccc taacgcgtag gatctttgtg aaggaacctt acttctgtgg
     6421 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     6481 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     6541 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     6601 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     6661 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     6721 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     6781 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt
     6841 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     6901 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     6961 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     7021 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     7081 tctggatcac tagtgatctg gccgggaagt tcctatactt tctagagaat aggaacttcc
     7141 tcgagaatca aaatgggcaa caaatgctgc agcaagcgac aggatcagga actggcactg
     7201 gcctatccca ctgggggcta caagaaatcc gactacacct ttggccagac gcacatcaac
     7261 agcagcggcg gcggcaacat gggcggcgtt cttggccaga agcataacaa cggtggctcg
     7321 ctggactcgc gctacacgcc cgatcccaat catcggggtc cgttgaaaat cggcggaaag
     7381 ggcggcgttg acatcatcag accacgcgga tcctccaaag gtgaagaact gtttaccgga
     7441 gtagtcccga tattggttga actcgacggc gatgtcaacg gtcataaatt cagtgtgtcc
     7501 ggcgagggtg agggcgacgc cacatacggt aagctgacgt tgaagttcat atgcaccacg
     7561 ggcaagctgc ccgtgccatg gccgacgttg gtcacgacgc tgacgtatgg tgtccagtgt
     7621 ttcagccgtt accccgatca tatgaagcag catgactttt tcaagtcggc gatgccggag
     7681 ggatacgttc aagagaggac cattttcttc aaggatgacg gcaactataa gacgcgagcg
     7741 gaggtgaaat ttgaaggcga cacactcgtt aaccgtattg agttgaaggg cattgatttt
     7801 aaggaggatg gcaacattct gggccataag ttggaataca actacaactc gcataatgtg
     7861 tatataatgg cagataagca gaaaaacgga ataaaggtta acttcaagat tcgccacaac
     7921 atagaggacg gttccgtgca acttgcagat cattaccaac agaacacacc cattggagat
     7981 ggcccagttc tcttgccaga caatcactac ctttccacac agtccgcgtt gagcaaggac
     8041 cccaatgaaa agcgggacca catggtgttg ctggagtttg tgaccgcagc tggtattaca
     8101 cacggcatgg acgagctgta caagcgggct aagagatcag gttctggagc accagtgaaa
     8161 cagactttga attttgacct tctcaagttg gcaggagacg tggagtccaa cccagggccc
     8221 atgagtactt ttgcagaagc agcgcatctt acaccgcatc aatgtgcgaa cgaaatcaac
     8281 gagattctgg aaagtgatac attcaacatt aatgcgaaag aaatacgcaa caagttggct
     8341 agtctctttt ccattcttac catgcaatcg ctgtccatcc gtagggaaat gaagattaac
     8401 acgtatcgta gctacaagtc cgcaatcgga aaatccctga gctttgacaa ggatgacaag
     8461 attatcaaat tcactgtacg gctgaggaag accgagagtc tgcagaaaga cattgaaagc
     8521 gcactcccgt cctacaaagt cgttgtttcg ccattcaaaa accaggaggt gtcgcttttc
     8581 gatcgatacg aggaaaccca caagtacgat gcgtccatgg tgggcctcca atttacgaac
     8641 attctcagca aggagaaaga catctggaaa atcgttagcc gaatcgcttg cttcttcgat
     8701 cagtcgtgcg tgacgaccac gaagcgagcg gagtaccgcc tgcttctgct gggagccgta
     8761 ggtaactgtt gccgttatag cgatctcaaa aatctggacc cccggacctt tgagatttac
     8821 aacaattcct tcttgggacc aatcgtgcgg gccacggtta cagagacaaa gagtcgcaca
     8881 gagcgatatg tgaactttta cccagtgaat ggtgattgcg atctgttgat ttccctctac
     8941 gattacctcc gagtttgctc cccgatcgag aagactgtgt cgagcaatcg gccgacaaac
     9001 cagacgcacc agttcctccc ggaatccctt gcgcggacct tcagccgctt cctgacccag
     9061 cacgttgacg agccagtctt caagatttgg aacggcccga agtcgcactt cggtcggcac
     9121 ctgatggcta ccttcttgtc gcgctccgaa aagggaaagt atgtttcctc cctgggcaac
     9181 tgggcaggtg accgcgaaat ccagagcgcc gtcgcgcgct cccactactc gcatggttcc
     9241 gttaccgttg acgaccgggt ctttgcattc atatcgggat tttacaaaga agccccgctg
     9301 ggttccgaga tttatgtgtt gaaggacccc tccaacaagc cactcagcag ggaggagctg
     9361 cttgaggagg agggcaactc gctcggaagt cccccactga gtcctccctc ctcgccccga
     9421 ctggtggccc agtcgttcag tgcacacccg tcgctgcaac tcttcgaaca gtggcacggc
     9481 atcatcagcg acgaggtcct gcaatttatt gcggaatata ggcgcaagca cgagctgcgg
     9541 agccagcgta cggtggttgc aggatcccat ggcttccctc cagaggtgga ggagcaagat
     9601 gatggcactc tccccatgag ctgcgctcaa gagagtggca tggatcgtca ccccgctgct
     9661 tgcgcctcgg ctcgcatcaa cgtgtaatct agaggatctt tgtgaaggaa ccttacttct
     9721 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     9781 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     9841 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     9901 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     9961 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
    10021 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
    10081 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatatttg atgtatagtg
    10141 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    10201 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    10261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10381 catgtctgga tcgatctggc cggccgttta aacgaattct tgaagacgaa agggcctcgt
    10441 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga ctcaggtggc
    10501 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
    10561 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
    10621 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
    10681 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
    10741 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
    10801 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
    10861 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
    10921 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
    10981 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
    11041 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
    11101 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
    11161 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    11221 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    11281 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    11341 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    11401 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    11461 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    11521 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    11581 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaa
//
