LOCUS       sequence-43400-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   White EA, Sowa ME, Tan MJ, Jeudy S, Hayes SD, Santha S, Munger K, 
            Harper JW, Howley PM
  TITLE     Systematic identification of interactions between host cell proteins
            and E7 oncoproteins from diverse human papillomaviruses.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Jan 9.
  PUBMED    22232672
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 Jan 9."
COMMENT     Sequence Label: p6641 MSCV-P C-FlagHA 18E7-Kozak
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..50)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    82..106
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(422..446)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(475..494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(476..494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(522..538)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(554..576)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tcgtaggggt atcctccagc ggccgacttg tcatcgtcgt ccttgtagtc ctcgagttcg
       61 aaccgcgggc cctctagatc aaccactttg tacaagaaag ttggctgctg ggatgcacac
      121 cacggacaca caaaggacag ggtgttcaga aacagctgct ggaatgctcg aaggtcgtct
      181 gctgagcttt ctactactag ctcaattctg gcttcacact tacaacacat acacaacatt
      241 gtgtgacgtt gtggttcggc tcgtcgggct ggtaaatgtt gatgattaac tccatctatt
      301 tcatcgtttt cttcctctga gtcgcttaat tgctcgtgac atagaaggtc aaccggaatt
      361 tcattttggg gctctaaatg caatacaatg tcttgcaatg ttgccttagg tccatgcatg
      421 gtgccaactt ttttgtacaa acttgttgat agcttaacta gccagcttgg gtctccctat
      481 agtgagtcgt attagatcta attccggcgc ctagagaagg agtgagggct ggataaaggg
      541 aggatcgagg cggggtcgaa cgaggaggtt caagggggag agacggggcg gatggaggaa
      601 gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca gggagggggg tcaaaagcca
      661 aggctttccc aggtcacgat gtagggggac ctggtctggg tgtccatgcg ggcccaggtg
      721 aaaagacctt gatcttaacc tgggtgaat
//
