LOCUS       sequence-434037-        8267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  catalytic domain of SOS2 fused with near ir fluorescent protein and 
            CEL tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8267)
  AUTHORS   Chung CI, Yang J, Shu X
  TITLE     Chemogenetic Minitool for Dissecting the Roles of Protein Phase 
            Separation.
  JOURNAL   ACS Cent Sci. 2023 Jul 7;9(7):1466-1479. doi: 
            10.1021/acscentsci.3c00251. eCollection 2023 Jul 26.
  PUBMED    37521779
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.3c00251"; journalName: "ACS Cent Sci"; date: 
            "2023-07-7- 7"; volume: "9"; issue: "7"; pages: "1466-1479"
COMMENT     Sequence Label: CEL-IFP2-SOScat
FEATURES             Location/Qualifiers
     source          1..8267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1295..2257
                     /codon_start=1
                     /product="bacteriophytochrome-based monomeric infrared 
                     fluorescent protein (Yu et al., 2014)"
                     /label=IFP2.0
                     /translation="MARDPQPFFPPLYLGGPEITTENCEREPIHIPGSIQPHGALLTAD
                     GHSGEVLQVSLNAATFLGQEPTVLRGQTLAALLPDQWPALQTALPPGCQDALQYRATLD
                     WPAAGHLSLTVHRVAELLILEFEPTEAWDSIGPHALRNAMFALESAPNLRALAEVATQT
                     VRELSGFDRVMLYKFAPDATGEVIAEARREGMQAYLGHRFPASTTPAQARALYTRHLLR
                     LTADTRAAAVPLDPVLNPQTNAPTPLGGAVLRATSPMHMQYLRNMGVGSSLSVSVVVGG
                     QLWGLIVCHHQTPYVLPPDLRTTLEYLGRLLSLQVQRKEA"
     primer_bind     complement(3858..3875)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3864..4088
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4134..4562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4221..4240)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4431..4452
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4571..4591)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4576..4905
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4756..4891
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4818..4837
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4972..5766
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5026..5045)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5636..5655
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5940..6061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5977..5996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6031..6050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6110..6126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6110..6126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6123..6145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6134..6150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6158..6164,6165..6182,6183..6188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6203..6224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6341..6358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6512..7100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6592..6611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7271..8062,8063..8131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7894..7913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8132..8236)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgtccct ttgctgtaaa caatgtcaag aaacagaaat
      961 aacaaccaaa aatgaaatat tcagtttatc cttatgtggg ccgatggcag cttatgtgaa
     1021 tcctcatgga tatgtgcatg agacacttac tgtgtataag gcttgcaact tgaatctgat
     1081 aggccggcct tctacagaac acagctggtt tcctgggtat gcctggactg ttgcccagtg
     1141 taagatctgt gcaagccata ttggatggaa gtttacggcc accaaaaaag acatgtcacc
     1201 tcaaaaattt tggggcttaa cgcgatctgc tctgttgccc acgatcccag acctcgagtc
     1261 cggactcaga tctggcagcg gtggaggcga tatcatggct cgggaccctc aacctttctt
     1321 ccctcctctg tatctcggag gaccagagat cactactgag aactgcgagc gggaacctat
     1381 acatatacca ggttctattc aacctcatgg agcactgctc acagccgatg gacattctgg
     1441 cgaggtgctg caagtttcac tcaacgcagc cactttcctg ggccaggaac caactgttct
     1501 gcggggacag accctggcag ccctgctgcc agaccagtgg cctgctctgc agactgccct
     1561 gcctccaggc tgccaggacg ccctgcagta tcgcgccacc ctggattggc ccgccgccgg
     1621 acacctgagc ctgaccgtgc atagggtggc cgagctgctg attctggaat tcgagcctac
     1681 tgaggcatgg gactctattg gaccccacgc tctgagaaat gcaatgttcg ctctggagag
     1741 tgctcctaat ctccgggcac tggctgaagt cgcaacccaa acagtccggg aactgtcagg
     1801 tttcgaccgg gtgatgctgt acaagtttgc accagacgca acaggagagg ttattgccga
     1861 agcaaggcgc gagggcatgc aggcttacct cgggcatagg tttcccgcat ccaccacccc
     1921 tgcacaagct agggccctct acacaagaca cctgctccgg ctgaccgcag acaccagggc
     1981 tgcagcagtg cccctcgacc ccgtgctgaa tccccagaca aatgctccta cacctctggg
     2041 cggagctgtc ctcagagcta catccccaat gcacatgcag tacctgagga atatgggagt
     2101 gggctcctcc ctgagcgtca gcgtcgtggt cggcggccag ctgtggggac tgattgtctg
     2161 ccaccatcag acaccctacg tgctgccacc agatctgcgg accaccctgg agtatctggg
     2221 gaggctcctg tccctgcagg tgcagaggaa agaagccgga tccggcagtg ctggtggtag
     2281 tgctggtggt agtgctggtg gtagtgctgg tggcagtgct ggtggtagtg ctggtggtag
     2341 tgctggtggc tctagaaatg agcaaccact gagattacca agtcctgaag tatatcgttt
     2401 tgtagtaaaa gactctgagg aaaacattgt ttttgaagac aacttgcaaa gtagaagtgg
     2461 catccccatt attaaaggag gaactgtagt gaaattaatt gaaaggttaa catatcatat
     2521 gtatgcagat cccaattttg ttcgtacttt tcttaccaca tatcgttcat tttgtaaacc
     2581 acaggaattg ctgagcttac tgattgaacg gtttgaaatt ccagagccag aacctactga
     2641 cgcagacaaa ttggcaatag agaaaggcga gcagccaatc agtgcagacc ttaaaagatt
     2701 tcgcaaggaa tatgtccaac cagtacaact taggatctta aatgtatttc ggcattgggt
     2761 tgaacatcat ttttatgact ttgaaagaga cttggaattg cttgaaagac tagaatcctt
     2821 catttcaagt gtaagaggga aagctatgaa aaaatgggta gagtcaattg ctaagatcat
     2881 caggaggaag aagcaagctc aggcaaacgg agtaagccat aatattacct ttgaaagtcc
     2941 acctccacca attgaatggc atatcagcaa accaggacag tttgaaacat ttgatctcat
     3001 gacacttcat ccaatagaaa ttgcacgtca gctgacactt ttggagtctg atctttacag
     3061 gaaagttcaa ccgtctgaac ttgtagggag tgtgtggacc aaagaagata aagaaataaa
     3121 ttctccaaat ttattaaaaa tgattcgcca taccacaaat ctcaccctct ggtttgaaaa
     3181 atgcattgtg gaagcagaaa attttgaaga acgggtggca gtactaagta gaattataga
     3241 aattctgcaa gtttttcaag atttgaataa tttcaatggc gtattggaga tagtcagtgc
     3301 agtaaattca gtgtcagtat acagactaga ccataccttt gaggcactgc aggaaaggaa
     3361 aaggaaaatt ttggacgaag ctgtggaatt aagtcaagat cactttaaaa aatacctagt
     3421 aaaacttaag tcaatcaatc caccttgtgt gccttttttt ggaatatatt taacaaatat
     3481 tctgaagacc gaagaaggga ataatgattt tttaaaaaag aaagggaaag atttaatcaa
     3541 tttcagtaag aggaggaaag tagctgaaat tactggagaa attcagcagt atcagaatca
     3601 gccttactgt ttacggatag aaccagatat gaggagattc tttgaaaacc ttaaccccat
     3661 gggaagtgca tctgaaaaag agtttacaga ttatttgttc aacaagtcac tagaaattga
     3721 acctcgaaac tgcaaacagc cacctcgatt tcctaggaaa tcaacttttt ccttaaaatc
     3781 tcctggaata aggcctaaca caggccgaca tggctctacc tcataagaat tcgggcccgt
     3841 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3901 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3961 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4021 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4081 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     4141 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4201 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4261 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4321 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4381 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4441 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4501 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4561 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     4621 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     4681 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     4741 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     4801 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     4861 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     4921 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4981 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5041 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5101 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5161 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5221 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5281 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5341 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5401 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5461 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5521 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5581 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5641 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5701 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5761 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5821 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5881 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5941 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6001 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6061 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6301 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     6361 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     6421 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     6481 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     6541 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     6601 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     6661 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     6721 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     6781 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     6841 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6901 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6961 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7021 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7081 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7141 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7201 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     7321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     7381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     7441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     7501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     7561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     7621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     7681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     7741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     7801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     7861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8101 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8161 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8221 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
