LOCUS       sequence-43404-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Mattingly RR, Macara IG
  TITLE     Phosphorylation-dependent activation of the Ras-GRF/CDC25Mm exchange
            factor by muscarinic receptors and G-protein beta gamma subunits.
  JOURNAL   Nature. 1996 Jul 18. 382(6588):268-72.
  PUBMED    8717044
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1996 Jul 18. 382(6588):268-72."
COMMENT     Sequence Label: pKH3
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..70)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(77..103)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(110..136)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(197..216)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(229..247)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(230..247)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(457..481)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(478..681)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(507..527)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tggcggccat cgactcagct atctatcgat tgaattcgga tccagcgtaa tctggaacat
       61 cgtatgggta agatccagcg taatctggaa catcgtatgg gtaagatcca gcgtaatctg
      121 gaacatcgta tgggtacatt ctagagtcga cctgcagaag cttagaaccg aggtgcagtt
      181 ggacctggga gtggacacct gtggagagaa aggcaaagtg gatgttattc tatagtgtca
      241 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
      301 agccaagggg gtgggcctat agactctata ggcggtactt acgtcactct tggcacgggg
      361 aatccgcgtt ccaatgcacc gttcccggcc gcggaggctg gatcggtccc ggtgtcttct
      421 atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaacgagc
      481 tctgcttata tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga
      541 gttgttacga cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac
      601 gtcaatgggg tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat
      661 gtactgccaa aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca
      721 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
      781 attga
//
