LOCUS       sequence-434047-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive expression of YAP/SPARK-ON (SFFV 
            promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Chung CI, Yang J, Shu X
  TITLE     Chemogenetic Minitool for Dissecting the Roles of Protein Phase 
            Separation.
  JOURNAL   ACS Cent Sci. 2023 Jul 7;9(7):1466-1479. doi: 
            10.1021/acscentsci.3c00251. eCollection 2023 Jul 26.
  PUBMED    37521779
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.3c00251"; journalName: "ACS Cent Sci"; date: 
            "2023-07-7- 7"; volume: "9"; issue: "7"; pages: "1466-1479"
COMMENT     Sequence Label: ZIF-NLS-EGFP*-HOTag6-T2A-CEL-EGFP-YAP
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             146..172
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             1100..1153
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1538..1540,1541..1543,1544..2254)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1583..1604)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1844..1863)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2191..2212
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3900..3917)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3906..4130
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4176..4604
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4263..4282)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4473..4494
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4613..4633)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4618..4947
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4798..4933
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4860..4879
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5014..5808
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5068..5087)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5678..5697
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5982..6103
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6019..6038)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6073..6092
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6152..6168)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6152..6168)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6165..6187)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6176..6192
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6200..6206,6207..6224,6225..6230))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6245..6266
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6383..6400)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6554..7142)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6634..6653)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7313..8104,8105..8173))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7936..7955
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8174..8278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8353..8372)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8544..8923
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8924..9127
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9078..9098
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9172..9191
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9172..9190
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgccaccatg actggagaac ggcccttcca gtgcaatcag tgcggggcct cattcaccca
       61 gaagggcaac ctgctccggc acatcaagct gcattccggg gagccgcggt ctggtctgcg
      121 ttctggttct ggtggtggta tcgatccagc agctaaaaga gtcaaattgg atatggtttc
      181 taaaggtgaa gaactgttta ctggtgttgt accgatcctg gttgaactgg acggcgacgt
      241 aaatggtcat aaattctccg tttctggtga gggcgagggt gatgcaactt atggcaagct
      301 gaccctgaaa ttcatttgca ccaccggaaa actgccggtt ccgtggccga ccctggtgac
      361 cactctgacc tttggcgtac agtgcttctc tcgctacccg gatcacatga aacagcacga
      421 ctttttcaaa tctgcgatgc cggaaggtta cgtacaagag cgtaccatct tcttcaaaga
      481 tgacggtaat tacaaaacaa gagcggaagt taaattcgag ggtgacaccc tggtgaaccg
      541 catcgaactg aaaggcatcg acttcaaaga ggatggcaac atcctgggcc acaaactgga
      601 atacaactac aactcccaca acgtgtacat catggctgat aagcagaaga acggcatcaa
      661 agcgaacttc aaaattcgtc acaacatcga agacggctct gttcaactgg cggatcacta
      721 ccagcagaac acgccgattg gcgacggccc ggttctgctg ccggataacc actatctgtc
      781 cacccagtct gccctgtcta aagacccgaa cgagaaacgt gaccatatgg tactgctgga
      841 atttgttacc gccgctggta tcaccctggg tatggacgaa ctgtacaaga ccggtggctc
      901 cgcaggtggt tccgctggtg gaagcgccgg tggttctgcg ggtggatctg ctggaggttc
      961 tgcaggagga agcgctggag gtgggcgcgc caccttgaga gaaatcgagg aattgttgag
     1021 gaaaatcatt gaagacagcg tgcgcagtgt ggccgagctg gaggacattg agaagtggct
     1081 gaagaaaatc ggcggccgcg agggcagagg aagtctgcta acatgcggtg acgtcgagga
     1141 gaatcctggc ccaaagcttg ccaccatgtc cctttgctgt aaacaatgtc aagaaacaga
     1201 aataacaacc aaaaatgaaa tattcagttt atccttatgt gggccgatgg cagcttatgt
     1261 gaatcctcat ggatatgtgc atgagacact tactgtgtat aaggcttgca acttgaatct
     1321 gataggccgg ccttctacag aacacagctg gtttcctggg tatgcctgga ctgttgccca
     1381 gtgtaagatc tgtgcaagcc atattggatg gaagtttacg gccaccaaaa aagacatgtc
     1441 acctcaaaaa ttttggggct taacgcgatc tgctctgttg cccacgatcc cagacctcga
     1501 gtccggactc agatctggca gcggtggagg cgatatcatg gtgagcaagg gcgaggagct
     1561 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1621 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1681 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1741 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1801 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1861 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1921 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1981 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcca acttcaagat
     2041 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2101 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     2161 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2221 cgggatcact ctcggcatgg acgagctgta caagggatcc ggcagtgctg gtggtagtgc
     2281 tggtggtagt gctggtggta gtgctggtgg cagtgctggt ggtagtgctg gtggtagtgc
     2341 tggtggctct agaatggatc ccgggcagca gccgccgcct caaccggccc cccagggcca
     2401 agggcagccg ccttcgcagc ccccgcaggg gcagggcccg ccgtccggac ccgggcaacc
     2461 ggcacccgcg gcgacccagg cggcgccgca ggcacccccc gccgggcatc agatcgtgca
     2521 cgtccgcggg gactcggaga ccgacctgga ggcgctcttc aacgccgtca tgaaccccaa
     2581 gacggccaac gtgccccaga ccgtgcccat gaggctccgg aagctgcccg actccttctt
     2641 caagccgccg gagcccaaat cccactcccg acaggccagt actgatgcag gcactgcagg
     2701 agccctgact ccacagcatg ttcgagctca ttcctctcca gcttctctgc agttgggagc
     2761 tgtttctcct gggacactga cccccactgg agtagtctct ggcccagcag ctacacccac
     2821 agctcagcat cttcgacagt cttcttttga gatacctgat gatgtacctc tgccagcagg
     2881 ttgggagatg gcaaagacat cttctggtca gagatacttc ttaaatcaca tcgatcagac
     2941 aacaacatgg caggacccca ggaaggccat gctgtcccag atgaacgtca cagcccccac
     3001 cagtccacca gtgcagcaga atatgatgaa ctcggcttca ggtcctcttc ctgatggatg
     3061 ggaacaagcc atgactcagg atggagaaat ttactatata aaccataaga acaagaccac
     3121 ctcttggcta gacccaaggc ttgaccctcg ttttgccatg aaccagagaa tcagtcagag
     3181 tgctccagtg aaacagccac cacccctggc tccccagagc ccacagggag gcgtcatggg
     3241 tggcagcaac tccaaccagc agcaacagat gcgactgcag caactgcaga tggagaagga
     3301 gaggctgcgg ctgaaacagc aagaactgct tcggcaggca atgcggaata tcaatcccag
     3361 cacagcaaat tctccaaaat gtcaggagtt agccctgcgt agccagttac caacactgga
     3421 gcaggatggt gggactcaaa atccagtgtc ttctcccggg atgtctcagg aattgagaac
     3481 aatgacgacc aatagctcag atcctttcct taacagtggc acctatcact ctcgagatga
     3541 gagtacagac agtggactaa gcatgagcag ctacagtgtc cctcgaaccc cagatgactt
     3601 cctgaacagt gtggatgaga tggatacagg tgatactatc aaccaaagca ccctgccctc
     3661 acagcagaac cgtttcccag actaccttga agccattcct gggacaaatg tggaccttgg
     3721 aacactggaa ggagatggaa tgaacataga aggagaggag ctgatgccaa gtctgcagga
     3781 agctttgagt tctgacatcc ttaatgacat ggagtctgtt ttggctgcca ccaagctaga
     3841 taaagaaagc tttcttacat ggttataaga attcgggccc gtttaaaccc gctgatcagc
     3901 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3961 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4021 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4081 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     4141 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4201 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4261 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4321 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4381 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4441 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4501 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4561 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4621 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4681 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4741 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4801 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4861 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4921 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4981 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5041 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5101 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5161 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5221 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5281 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5341 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5401 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5461 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5521 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5581 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5641 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5701 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5761 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5821 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5881 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5941 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     6001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     6121 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6181 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6241 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6301 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6361 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6421 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6481 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6541 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6601 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6661 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6721 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6781 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6841 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6901 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6961 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7021 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7081 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7141 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7201 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7261 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7321 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7381 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7441 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7501 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7561 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7621 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7681 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7741 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7801 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7861 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7921 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7981 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8041 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8101 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8161 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8221 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8281 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     8341 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     8401 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     8461 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     8521 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
     8581 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     8641 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     8701 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     8761 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     8821 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     8881 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     8941 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     9001 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     9061 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     9121 cagagctctc tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac
     9181 tcactatagg gagacccaag ctggctag
//
