LOCUS       sequence-434074-        4111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hFGF1 expression in mammalian cells. pAAV vector. Reporter plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Baloban M, Kasatkina LA, Verkhusha VV
  TITLE     iLight2: A near-infrared optogenetic tool for gene transcription 
            with low background activation.
  JOURNAL   Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993.
  PUBMED    38647395
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2024-05"; volume: "33(5):e4993. doi"; pages: " 
            10.1002/pro.4993"
COMMENT     Sequence Label: pAAV-G12-hFGF1
FEATURES             Location/Qualifiers
     source          1..4111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..192
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     174..191
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             892..903
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(1043..1063)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     1245..1264
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   1374..1514
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1374..1503
                     /label=AAV2 ITR
     rep_origin      1589..2044
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1676..1695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1886..1907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2061..2080)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2180..2202
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2240..2258)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2326..2430
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2431..2499,2500..3291)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2649..2668)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3462..4050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3951..3970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgaaga tgctgcacac ttgtgggcta gccttatgta tcatacacat acgatttagg
      181 tgacactata gaactcgagc agctgaagct tcggagtact gtcctccgag cggagtactg
      241 tcctccgagc ggagtactgt cctccgagtc gactctagcg gagtactgtc ctccgagcgg
      301 agtactgtcc tccgagcgga gtactgtcct ccgagtcgac tctagcggag tactgtcctc
      361 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agtcgactct agcggagtac
      421 tgtcctccga gcggagtact gtcctccggc tagtagcgga gactctagag ggtatataat
      481 ggatccccgg gtaccggtcg ccaccatggc tgaaggggaa atcaccacct tcacagccct
      541 gaccgagaag tttaatctgc ctccagggaa ttacaagaag cccaaactcc tctactgtag
      601 caacgggggc cacttcctga ggatccttcc ggatggcaca gtggatggga caagggacag
      661 gagcgaccag cacattcagc tgcagctcag tgcggaaagc gtgggggagg tgtatataaa
      721 gagtaccgag actggccagt acttggccat ggacaccgac gggcttttat acggctcaca
      781 gacaccaaat gaggaatgtt tgttcctgga aaggctggag gagaaccatt acaacaccta
      841 tatatccaag aagcatgcag agaagaattg gtttgttggc ctcaagaaga atgggagctg
      901 caaacgcggt cctcggactc actatggcca gaaagcaatc ttgtttctcc ccctgccagt
      961 ctcttctgat tgagaattcc gctcgagata atcaacctct ggattacaaa atttgtgaaa
     1021 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     1081 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     1141 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     1201 gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta
     1261 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1321 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcggc cgcaggaacc
     1381 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     1441 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     1501 cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     1561 acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg
     1621 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct
     1681 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1741 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1801 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1861 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1921 tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta
     1981 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca
     2041 attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     2101 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     2161 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     2221 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     2281 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2341 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2401 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     2461 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     2521 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     2581 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     2641 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     2701 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     2761 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     2821 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     2881 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     2941 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     3001 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     3061 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     3121 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     3181 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     3241 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     3301 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     3361 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     3421 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3481 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3541 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3601 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3661 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3721 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3781 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3841 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3901 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3961 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     4021 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     4081 ctggcctttt gctggccttt tgctcacatg t
//
