LOCUS       sequence-434075-        4108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mFGF2 expression in mammlian cells. pAAV vector. Reporter plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Baloban M, Kasatkina LA, Verkhusha VV
  TITLE     iLight2: A near-infrared optogenetic tool for gene transcription 
            with low background activation.
  JOURNAL   Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993.
  PUBMED    38647395
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2024-05"; volume: "33(5):e4993. doi"; pages: " 
            10.1002/pro.4993"
COMMENT     Sequence Label: pAAV-G12-mFGF2
FEATURES             Location/Qualifiers
     source          1..4108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..192
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     174..191
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1040..1060)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     1242..1261
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   1371..1511
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   1371..1500
                     /label=AAV2 ITR
     rep_origin      1586..2041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1673..1692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1883..1904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2058..2077)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2177..2199
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2237..2255)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2323..2427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2428..2496,2497..3288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2646..2665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3459..4047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3948..3967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgaaga tgctgcacac ttgtgggcta gccttatgta tcatacacat acgatttagg
      181 tgacactata gaactcgagc agctgaagct tcggagtact gtcctccgag cggagtactg
      241 tcctccgagc ggagtactgt cctccgagtc gactctagcg gagtactgtc ctccgagcgg
      301 agtactgtcc tccgagcgga gtactgtcct ccgagtcgac tctagcggag tactgtcctc
      361 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agtcgactct agcggagtac
      421 tgtcctccga gcggagtact gtcctccggc tagtagcgga gactctagag ggtatataat
      481 ggatccccgg gtaccggtcg ccaccatggc tgccagcggc atcacctcgc ttcccgcact
      541 gccggaggac ggcggcgccg ccttcccacc aggccacttc aaggacccca agcggctcta
      601 ctgcaagaac ggcggcttct tcctgcgcat ccatcccgac ggccgcgtgg atggcgtccg
      661 cgagaagagc gacccacacg tcaaactaca actccaagca gaagagagag gagttgtgtc
      721 tatcaaggga gtgtgtgcca accggtacct tgctatgaag gaagatggac ggctgctggc
      781 ttctaagtgt gttacagaag agtgtttctt ctttgaacga ctggaatcta ataactacaa
      841 tacttaccgg tcacggaaat actccagttg gtatgtggca ctgaaacgaa ctgggcagta
      901 taaactcgga tccaaaacgg gacctggaca gaaggccata ctgtttcttc caatgtctgc
      961 taagagctga gaattccgct cgagataatc aacctctgga ttacaaaatt tgtgaaagat
     1021 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     1081 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     1141 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     1201 ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt gatgctattg
     1261 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1321 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcggccgc aggaacccct
     1381 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
     1441 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
     1501 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     1561 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
     1621 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
     1681 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     1741 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     1801 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     1861 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
     1921 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
     1981 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
     2041 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     2101 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2161 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2221 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2281 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2341 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2401 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2461 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2521 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2581 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2641 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     2701 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     2761 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     2821 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     2881 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     2941 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3001 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3061 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3121 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3181 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3241 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3301 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3361 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3601 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     3901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     3961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4081 gccttttgct ggccttttgc tcacatgt
//
