LOCUS       sequence-434076-        5296 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AkaLuc expression in mammlian cells. pAAV vector. Reporter plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Baloban M, Kasatkina LA, Verkhusha VV
  TITLE     iLight2: A near-infrared optogenetic tool for gene transcription 
            with low background activation.
  JOURNAL   Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993.
  PUBMED    38647395
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci."; date: "2024-05"; volume: "33(5):e4993. doi"; pages: " 
            10.1002/pro.4993"
COMMENT     Sequence Label: pAAV-G12-AkaLuc
FEATURES             Location/Qualifiers
     source          1..5296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        174..192
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     174..191
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2228..2248)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     2430..2449
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2559..2699
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2559..2688
                     /label=AAV2 ITR
     rep_origin      2774..3229
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2861..2880)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3071..3092
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3246..3265)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3365..3387
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3425..3443)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3511..3615
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3616..3684,3685..4476)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3834..3853)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4647..5235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5136..5155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccggc
       61 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg
      121 cggccgaaga tgctgcacac ttgtgggcta gccttatgta tcatacacat acgatttagg
      181 tgacactata gaactcgagc agctgaagct tcggagtact gtcctccgag cggagtactg
      241 tcctccgagc ggagtactgt cctccgagtc gactctagcg gagtactgtc ctccgagcgg
      301 agtactgtcc tccgagcgga gtactgtcct ccgagtcgac tctagcggag tactgtcctc
      361 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agtcgactct agcggagtac
      421 tgtcctccga gcggagtact gtcctccggc tagtagcgga gactctagag ggtatataat
      481 ggatccccgg gtaccggtcg ccaccatgga agatgccaaa aacattaaga agggcccagc
      541 gccgttctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg
      601 ctacgccctg gtgcccggcg ccatcgcctt taccgacgca catattcagg tggacgttac
      661 ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaggc gctatgggct
      721 gaatacaaac catcggatcg tggtgtgcag cgagaatagc tcgcagttct tcatgcccgt
      781 gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg
      841 cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg
      901 gctgcgaaag gtcctcaacg tgcaaaagaa gctaccgatc atacgaaaga tcatcatcat
      961 ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt
     1021 gccacccagc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat
     1081 cgccctgatc atgaacagta gtggtagtac aggattaccc aagggcgtag ccctaccgca
     1141 ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggct accagaacat
     1201 ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac
     1261 gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct
     1321 attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt
     1381 tagctgcctc gctaagagca ctctcatcga caagtacgac ctaagcagct tgcgcgagat
     1441 cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttccg
     1501 cctaccaggc atccgccagg gctatggcct gacagaaaca accaacgccg tcatgatcac
     1561 ccccgagggg gaccgtaagc ctggctcagt aggcaaggtg gtgcccttct tcgaggctaa
     1621 ggtggtagac ttggtcaccg gtaagacact gggtgtgaac cagcgcggtg agctgtgcgt
     1681 ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacga acgctctcat
     1741 cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg acgagcactt
     1801 cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg tagccccagc
     1861 cgaactggag ggcatcctgc tgcaacaccc ctacatcttc gacgccggag tcgccggcct
     1921 gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgttggaac acggtaaaac
     1981 catgaccgag aaagagatcg tggactatgt ggccagccag gttacaaccg ccaagaagct
     2041 gcgcggtggt gttgtgtttg tggatgaagt ccctagagga tcgaccggca agttagacgc
     2101 ccgcaagatc cgcgagattc tcactaaggc caagaaggac ggcaagatcg ccgtgtaaga
     2161 attccgctcg agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     2221 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     2281 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     2341 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2401 gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2461 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     2521 ttcaggggga gatgtgggag gttttttaaa gcggccgcag gaacccctag tgatggagtt
     2581 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     2641 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     2701 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     2761 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     2821 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     2881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     2941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     3121 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     3181 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3241 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3301 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3361 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3421 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3481 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3541 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     3601 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     3661 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     3721 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     3781 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     3841 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     3901 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     3961 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4021 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4081 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4141 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4201 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4261 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4321 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4381 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4441 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4501 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4561 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4621 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4681 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4741 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4801 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4861 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4921 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4981 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5041 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5101 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5161 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5221 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5281 ccttttgctc acatgt
//
