LOCUS       sequence-434145-        1877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Minimal plasmid; contains only ori and ampR, destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   McGee AV, Liu YV, Griffith AL, Szegletes ZM, Wen B, Kraus C, Miller 
            NW, Steger RJ, Escude Velasco B, Bosch JA, Zirin JD, Viswanatha R, 
            Sontheimer EJ, Goodale A, Greene MA, Green TM, Doench JG
  TITLE     Modular vector assembly enables rapid assessment of emerging CRISPR 
            technologies.
  JOURNAL   Cell Genom. 2024 Mar 13;4(3):100519. doi: 
            10.1016/j.xgen.2024.100519.
  PUBMED    38484704
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Genom."; date: "2024-03-13"; volume: "4(3):100519. doi"; pages: " 
            10.1016/j.xgen.2024.100519"
COMMENT     Sequence Label: pRDA_791
FEATURES             Location/Qualifiers
     source          1..1877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        243..347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(348..416,417..1208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(566..585)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(1379..1877,1..89)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(1867..1877,1..9)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
       61 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      121 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtca
      181 gaacgtcttc tgactcgagg acgaagacgc attctttagg tggcactttt cggggaaatg
      241 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      301 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      361 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      421 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      481 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      541 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      601 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      661 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      721 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      781 agctaaccgc ttttttgcac aacatggggg accatgtaac tcgccttgat cgttgggaac
      841 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      901 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      961 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1021 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1081 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1141 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1201 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1261 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1321 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1381 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1441 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1501 gcagagcgca gatacaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1561 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1621 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1681 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1741 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1801 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1861 tccaggggga aacgcct
//
