LOCUS       sequence-434218-        9058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminally EGFP-tagged IFT80 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9058)
  TITLE     Nakayama Lab Plasmids 2024
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG-EGFP-N-IFT80
FEATURES             Location/Qualifiers
     source          1..9058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      4114..4123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4120..4122,4123..4125,4126..4839)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4120..4122,4123..4125,4126..4836)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4165..4186)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4426..4445)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4773..4794
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4927..4946)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4992..5047
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5046..5065)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5408..5424)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5408..5424)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5421..5443)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5432..5448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5456..5462,5463..5480,5481..5486))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5501..5522
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5581..5776
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5627..5762
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5782..5916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5819..5838)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5873..5892
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5984..6001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6155..6743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6235..6254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6914..7705,7706..7774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7537..7556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7775..7879)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8191..8994
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8254..8273)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8864..8883
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9001..9049
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattt gctagtttga
     1741 ctcagatcca ccatgagact aaagatatct cttttaaaag aaccaaagca tcaagaatta
     1801 gtaagctgtg tgggctggac tactgctgaa gagctgtatt catgtagtga tgatcaccag
     1861 atagtgaagt ggaacttgtt aaccagtgaa acaactcaaa tagtaaagct tcctgatgat
     1921 atttacccta ttgattttca ctggtttcca aaaagtttgg gtgtaaagaa acaaacccag
     1981 gcagaaagct ttgtcctcac aagttctgat ggtaaatttc atctgatttc caagttagga
     2041 agagtggaaa aaagtgtaga agctcactgt ggagcagtac ttgcaggaag atggaattat
     2101 gaaggaacag cattagttac agttggagaa gatggacaaa taaaaatttg gtcaaagact
     2161 gggatgctta gatcaacttt agctcagcaa ggaacaccag tgtattcagt agcgtggggc
     2221 cctgattcag aaaaggttct ttatacagca ggcaagcagc taatcattaa acctcttcaa
     2281 ccaaatgcta aagttttgca gtggaaagct catgatggca ttattttaaa agtagattgg
     2341 aactcggtca atgatcttat tttatctgct ggtgaagact gtaaatataa ggtatgggat
     2401 agttacggcc gcccactgta caattcacaa cctcatgagc atcccattac ttcagttgcc
     2461 tgggctccag atggagaatt atttgctgtt ggatcgtttc atactttacg cttgtgtgat
     2521 aaaactgggt ggtcatatgc attagaaaaa cccaacactg gcagcatatt taatattgca
     2581 tggtctatcg atggcactca gattgctgga gcctgtggaa atggacatgt cgtttttgca
     2641 catgtggtgg aacaacattg ggagtggaaa aattttcaag taacattaac gaaaagaaga
     2701 gccatgcagg ttcgtaatgt tcttaatgat gcagtggatt tactggaatt ccgtgataga
     2761 gtcattaaag catctttgaa ctatgcacac ttagttgttt caacgtctct tcaatgttac
     2821 gtgttctcca cgaagaactg gaacacacca attatatttg atctcaaaga aggaactgtt
     2881 agtttgattc tgcaggcaga aagacatttt cttcttgtag atggtagtag tatctattta
     2941 tattcatatg aagggcgctt tatttcatct ccaaaatttc ctggaatgag aacagatatt
     3001 ctgaatgcac agactgtgtc tttgagtaat gataccatag caataagaga caaagctgat
     3061 gaaaaaataa tcttcctctt tgaggcatca accggaaagc cgttaggtga tggaaagttt
     3121 ctttctcata agaatgaaat cttggaaatt gctctggatc aaaaaggact taccaatgat
     3181 agaaaaattg ctttcattga taaaaataga gatctctgta tcacttctgt gaaacgattt
     3241 gggaaggaag aacaaattat caagcttgga acaatggtgc atactttggc atggaacgat
     3301 acatgcaata tcctttgtgg acttcaagat actcgattta tagtgtggta ttaccccaat
     3361 acagtttatg tggacagaga cattttgcct aaaacattat atgaaaggga tgcaagtgaa
     3421 tttagtaaaa atccccatat tgtgagtttt gttggaaatc aagtaactat tagaagagct
     3481 gatggctccc tggttcacat cagcataaca ccatatcctg ctattctcca tgaatatgta
     3541 agcagttcaa aatgggaaga tgctgtgaga ctttgtcgct ttgttaagga gcaaaccatg
     3601 tgggcttgtc tagctgctat ggcagttgct aatcgagata tgactactgc agaaatagcc
     3661 tatgcagcaa ttggtgaaat tgataaggtt cagtacatca attctataaa aaatcttcca
     3721 tctaaagaat caaaaatggc ccacatacta ctgtttagtg ggaacataca ggaggctgaa
     3781 atagtacttc ttcaggctgg ccttgtttat caagcaatcc agatcaatat taatctctac
     3841 aactgggaaa gggcactgga attggctgta aaatacaaaa cacatgttga tacagttctt
     3901 gcttaccgtc aaaagttttt ggagacattt ggtaaacagg aaactaataa acgatacttg
     3961 cattatgcag aaggtctcca aatagattgg gagaaaatca aagccaaaat tgagatggaa
     4021 attacaaaag aaagagagca atcatcaagc agccaatcca gcaagagtat aggtttaaag
     4081 cccctcgagg gtaccgcggg cccgggatcc atcgccacca tggtgagcaa gggcgaggag
     4141 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     4201 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     4261 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     4321 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     4381 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     4441 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     4501 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     4561 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     4621 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     4681 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     4741 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     4801 gccgggatca ctctcggcat ggacgagctg tacaagtaac aattcactcc tcaggtgcag
     4861 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
     4921 gatcgatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct
     4981 gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg
     5041 tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt
     5101 ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata
     5161 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     5221 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     5281 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     5341 agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt
     5401 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5461 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5521 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     5581 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     5641 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     5701 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     5761 cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc
     5821 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5881 tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac
     5941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg agcagtgtgg
     7921 ttttcaagag gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca cccaatgtcg
     7981 agcagtgtgg ttttgcaaga ggaagcaaaa agcctctcca cccaggcctg gaatgtttcc
     8041 acccaatgtc gagcaaaccc cgcccagcgt cttgtcattg gcgaattaat tcgaacacgc
     8101 agatgcagtc ggggcggcgc ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg
     8161 cctcgaacac cgagcgaccc tgcagccaat atgggatcgg ccattgaaca agatggattg
     8221 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     8281 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     8341 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     8401 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     8461 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     8521 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     8581 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     8641 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     8701 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc
     8761 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     8821 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     8881 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     8941 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat
     9001 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttcg gatccgtc
//
