LOCUS       sequence-434222-       11197 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally EGFP-tagged IFT172 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11197)
  TITLE     Nakayama Lab Plasmids 2024
REFERENCE   2  (bases 1 to 11197)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG-EGFP-C-IFT172
FEATURES             Location/Qualifiers
     source          1..11197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..661
                     /label=chicken beta-actin promoter
     intron          662..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1734..1743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1740..1742,1743..1745,1746..2456)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1740..1742,1743..1745,1746..2456)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1785..1806)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2046..2065)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2393..2414
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7863..7882)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7928..7983
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7982..8001)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8338..8357)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8339..8357)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8367..8384)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8367..8383)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8376..8398)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8525..8980
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8612..8631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8822..8843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9006..9110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9111..9179,9180..9971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9329..9348)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10142..10730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10631..10650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10884..10901
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11018..11039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11054..11059,11060..11077,11078..11084)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11092..11108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11097..11119
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11116..11132
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11116..11132
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11151..11171
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11153..11171
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      421 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      481 gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaatcaccg gtcgccacca
     1741 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     1801 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     1861 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     1921 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     1981 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2041 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2101 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2161 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     2221 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     2281 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     2341 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     2401 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg
     2461 gactcagatc tcgagctcaa gcttccatgc acttgaagca cctgaggacc ctgctgagcc
     2521 ctcaggatgg agctgcaaag gtgacctgca tggcttggtc ccagaacaat gccaaatttg
     2581 ctgtctgcac agtggaccga gtggtcttgc tgtatgatga acatggagaa cggagagata
     2641 aattctccac caaaccagct gacatgaagt atggcaggaa gagctatatg gtgaagggca
     2701 tggctttttc tcctgattcc actaaaattg ccataggaca gactgacaac atcatctatg
     2761 tctacaagat tggagaagat tggggtgaca agaaagtcat ctgcaacaag ttcatccaga
     2821 cgagtgctgt cacttgtctg caatggccgg cagaatacat cattgtcttt ggactggctg
     2881 aagggaaggt tcgtttagca aacaccaaaa ctaataaatc atctaccatc tatgggacag
     2941 agtcttacgt ggtgtccctg acaacaaatt gctctgggaa aggaattctc tctggtcatg
     3001 cagatggtac catcgttagg tatttctttg atgatgaagg ctctggagag tcacagggga
     3061 agttggttaa ccacccgtgt ccaccctatg ccttggcatg ggcaaccaat agcatcgtgg
     3121 ctgcaggctg tgatcggaaa attgtagcct atggaaaaga aggtcacatg ctacaaactt
     3181 ttgattatag ccgtgaccct caggagcggg agttcaccac agctgtatca agtcctgggg
     3241 gccagtctgt tgtgctagga agttatgaca ggcttcgggt gttcaactgg atccctcgaa
     3301 gaagcatctg ggaagaggca aagcccaagg agattaccaa tttatacacc atcactgcct
     3361 tggcctggaa gcgggatggc tcacggctct gtgtgggcac actatgtggt ggggtggaac
     3421 agtttgactg ctgcctccga aggagtattt acaagaacaa gtttgagttg acgtatgtgg
     3481 gacctagcca ggtgattgtg aagaacctgt catcaggaac ccgagtggtg ctcaagtcac
     3541 actatggcta tgaggtggaa gaggtgaaaa tcctaggaaa ggaacgttac ttggtggctc
     3601 acacatcaga aacactgctg ctgggggacc tgaacactaa tcggcttagt gagatagcct
     3661 ggcaaggatc tggtggcaat gagaagtatt tctttgaaaa tgagaatgta tgcatgatct
     3721 tcaatgccgg agagctaacc ctggtggaat atgggaataa tgacaccctg ggttctgtac
     3781 gcactgaatt catgaacccc cacctcatca gtgttcgtat taatgagagg tgtcagcgag
     3841 gaacagaaga taataagaaa ttggcttatc ttattgatat taagactatt gctatagtgg
     3901 atctgattgg tggctacaac attggcaccg tcagccatga gagccgtgtg gattggctgg
     3961 aacttaatga gactggacac aagctcctct tcagggaccg gaaacttcgt ttgcatctgt
     4021 atgatattga aagctgctct aagacaatga tcctcaactt ctgctcctat atgcagtggg
     4081 tcccaggaag tgacgtgctg gtagctcaga accgaaacag tctgtgtgta tggtacaaca
     4141 ttgaggcacc tgagagagtc accatgttca ctattagggg tgatgttata ggtctggagc
     4201 ggggcggggg aaagaccgag gtgatggtga tggaaggtgt gactactgtt gcctacacat
     4261 tggatgaggg cctcatcgag tttggaacag ccattgatga tggcaactac atccgggcaa
     4321 cagccttctt agagactctg gaaatgaccc cagaaacaga ggcaatgtgg aaaaccttga
     4381 gtaaactggc actagaggca aggcaactac acattgcgga gaggtgcttt tctgctttgg
     4441 gccaagtagc aaaagctcga ttcctgcatg agaccaatga gattgcagat caagtatccc
     4501 gggaatatgg cggagaagga acagactttt atcaggtccg agcacgtcta gccatgctgg
     4561 aaaagaacta caaactggct gaaatgatct ttttggaaca gaatgctgtg gaggaggcca
     4621 tgggcatgta ccaggagcta caccgttggg atgagtgtat cgctgtggct gaagccaagg
     4681 ggcacccagc cctggagaag ctacgtcgta gttactacca gtggctgatg gacacacagc
     4741 aagaggagcg agcaggtgaa ctacaggaga gccaagggga tgggctagca gccatcagcc
     4801 tctacctcaa agctgggctc cctgccaaag ctgctcggct ggtgctgacc cgagaggaac
     4861 tgctagccaa cacagagctg gtagaacaca tcactgcagc ccttatcaag ggggaactct
     4921 acgaaagggc aggtgatctc tttgagaaga ttcacaatcc acagaaggcc ctggagtgct
     4981 accgtaaagg caacgcattc atgaaagcgg tagagctggc tcgattggcc ttcccagtgg
     5041 aggtggtgaa actagaggag gcatgggggg accacctggt gcagcagaag cagcttgatg
     5101 cagccattaa tcactacatc gaagccaggt gctccattaa ggcaattgag gccgccctgg
     5161 gtgcccgcca gtggaagaag gcaatttata tattagatct acaggaccgg aacactgcat
     5221 ccaaatacta tcctctcgtg gcccaacact atgcatccct gcaggagtat gagattgctg
     5281 aggagctcta tactaaggga gatcggacaa aagatgccat agacatgtac acccaggctg
     5341 gtcgttggga acaagcccac aagctggcga tgaaatgcat gagaccagaa gatgtgtcag
     5401 tgctatacat cactcaggcc caggaaatgg agaagcaggg caagtaccgt gaggctgaaa
     5461 ggctatatgt gacagtacaa gagcctgatc ttgccatcac catgtacaaa aagcacaagt
     5521 tgtatgatga catgatccgc ctggtaggga agcaccatcc agatctcctc agtgatacac
     5581 acctacatct gggcaaggag ctggaggctg aaggccgact acaggaggct gagtaccact
     5641 acctcgaggc ccaggaatgg aaggcaacag tgaacatgta ccgggccagt gggctttggg
     5701 aagaggccta cagggtggcc agaactcaag gaggggctaa tgcccacaaa cacgtggcct
     5761 atctgtgggc aaagagcctg ggaggagagg ctgcagttag actgcttaat aagctgggac
     5821 tcctggaagc tgctgttgac cacgctgcag acaattgctc ctttgaattt gcgtttgaac
     5881 tctctcggct ggccctcaag cacaaaaccc ccgaggttca tctcaaatat gctatgttcc
     5941 tggaggatga gggtaaattc gaagaggctg aagctgaatt catcagagct ggtaaaccca
     6001 aggaggcagt cctcatgttt gtccataacc aggattggga ggcagctcag cgtgtggctg
     6061 aggctcacga ccctgacagt gtcgccgagg tgcttgtggg acaggcccgg ggggccttgg
     6121 aggagaagga ctttcagaaa gcagaagggc tgctgctccg ggcccagaga ccaggcctgg
     6181 ccctcaatta ttataaggag gctggattat ggagtgacgc tctgcgcatc tgcaaggact
     6241 atgtgcccag ccagctggag gctctgcagg aagaatatga gcgggaagct actaagaagg
     6301 gggccagggg tgtggaggga tttgtggaac aagctcgaca ctgggagcag gctggagagt
     6361 acagccgtgc cgtggactgc tacctcaaag tgcgagactc tggaaacagc ggcctggcgg
     6421 agaagtgctg gatgaaggca gctgaactct ccatcaagtt tctgcctccc caacgtaata
     6481 tggaagtcgt tctggctgta ggaccccagc tgattggaat tggaaagcac agtgcagctg
     6541 cagagctcta tctgaatctg gaccttgtca aggaagcaat cgatgctttc atcgagggtg
     6601 aggagtggaa caaggcgaag cgtgtagcta aggagttaga tcccaggtat gaagactatg
     6661 tggaccagca ttataaagag ttcctcaaga atcagggcaa agtggactcg ctggtgggtg
     6721 tggatgtgat agctgctttg gacctgtatg tggagcaggg ccagtgggac aagtgcattg
     6781 aaacagctac caagcagaac tacaagattc tgcacaagta tgtggctttg tatgcaactc
     6841 acttgatccg ggagggtagc tctgcccagg cattggccct gtatgtacag cacggagccc
     6901 ctgctaaccc acagaacttc aatatctaca aaaggatctt cactgacatg gtgagctctc
     6961 ctggaaccaa ctgtgccgag gcctatcata gctgggctga tcttcgagat gtcctcttca
     7021 acctgtgtga aaacctggtg aagtccagtg aggcaaactc tccagcccat gaggagttca
     7081 agacgatgct gctgatcgct cattactatg ccacgcgctc tgcagcccag agtgtcaaac
     7141 agctggaaac cgtggctgcc aggctttctg tttcactctt gcgtcacacc cagctactac
     7201 ctgtagacaa agccttctat gaagcaggca ttgctgccaa ggcagttggc tgggataaca
     7261 tggcattcat cttcctcaat cgctttttgg acctgaccga tgcaatcgag gaagggactc
     7321 tagatggcct tgaccactct gattttcagg atacagacat tccctttgag gtgccactcc
     7381 cagctaagca gcatgtaccg gaggctgaga gagaagaggt tcgagactgg gtgcttacag
     7441 tctccatgga ccagcggctg gagcaggttc tgcctcggga tgagcgtggc gcctacgagg
     7501 cctccctagt ggcagcgagc actggtgttc gagccctgcc ctgccttatt acaggatacc
     7561 ccattctgag gaacaaaatt gaatttaagc ggccagggaa ggctgctaac aaggacaact
     7621 ggaataaatt ccttatggcc atcaagacct cccacagccc agtgtgccag gacgtgctga
     7681 aattcatcag tcagtggtgt ggagggctcc ccagcaccag cttttccttt cagtaggtcg
     7741 acggtaccgc gggcccggga tccaccggat ctagataact gatcgactcc tcaggtgcag
     7801 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactgg
     7861 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
     7921 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
     7981 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
     8041 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
     8101 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
     8161 acttgaggtt agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta
     8221 aaattttcct tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc
     8281 atagctgtcc ctcttctctt atgaagatcc ctcgacttaa ttaaggtacc caattcgccc
     8341 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     8401 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     8461 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     8521 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     8581 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     8641 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     8701 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     8761 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     8821 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     8881 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     8941 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     9001 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9061 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9121 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9181 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     9241 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     9301 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     9361 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     9421 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     9481 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     9541 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9601 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9661 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9721 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9781 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9841 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9901 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9961 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10021 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10081 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10141 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10201 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    10261 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    10321 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    10381 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    10441 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    10501 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    10561 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10621 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10681 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10741 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10801 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10861 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10921 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    10981 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    11041 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    11101 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
    11161 ctcactaaag ggaacaaaag ctggagctca tcgatac
//
