LOCUS       sequence-434233-       10341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses C-terminally EGFP-tagged IFT140 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10341)
  TITLE     Nakayama Lab Plasmids 2024
REFERENCE   2  (bases 1 to 10341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG2-EGFP-C-IFT140
FEATURES             Location/Qualifiers
     source          1..10341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        3..382
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..660
                     /label=chicken beta-actin promoter
     intron          661..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1601..1623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1733..1742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1739..1741,1742..1744,1745..2455)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1739..1741,1742..1744,1745..2455)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1784..1805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2045..2064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2392..2413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7007..7026)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7072..7127
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7126..7145)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7482..7501)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7483..7501)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7511..7528)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7511..7527)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7520..7542)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7669..8124
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7756..7775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7966..7987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8150..8254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8255..8323,8324..9115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8473..8492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9286..9874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9775..9794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10028..10045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10162..10183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10198..10203,10204..10221,10222..10228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10236..10252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10241..10263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10260..10276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10260..10276
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10295..10315
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10297..10315
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
       61 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      121 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      181 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      241 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      301 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      361 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat
      421 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc
      481 gatgggggcg gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
      541 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
      601 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
      661 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
      721 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
      781 ccgggctgta attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa
      841 agccttgagg ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc
      901 gtgtgtgtgt gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc
      961 tgcgggcgcg gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg
     1021 ggcggtgccc cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac
     1141 ccccctcccc gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt
     1201 ggcgcggggc tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg
     1261 gggccgcctc gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc
     1321 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1381 cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc
     1441 ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag
     1501 ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg
     1561 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1621 accggcggct ctagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaattaccgg tcgccaccat
     1741 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1801 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1861 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1921 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1981 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2041 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2101 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2161 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2221 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2281 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2341 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2401 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg
     2461 actcagatct cgagctcaag cttcgaattc tatggccctc tattatgacc accagataga
     2521 agccccggat gcagcagggt caccctcatt tatcagctgg caccctgtcc atccattctt
     2581 ggcagttgct tacatcagca caacctcaac aggcagcgtg gatatttacc tggagcaagg
     2641 ggagtgcgtg ccagatacac acgtcgagag gccgttccgg gttgcttccc tgtgctggca
     2701 cccgacgcgg ctggtgctgg ctgtgggctg ggagactgga gaagtgacgg tgtttaacaa
     2761 gcaggacaag gagcagcaca cgatgcccct gacacacaca gccgacatca ccgtgctccg
     2821 ttggagcccc agtggaaact gcctgctgtc tggggacagg cttggtgtct tgctcttgtg
     2881 gaggttggac caaaggggcc gagtgcaagg gacgcctctg ctgaaacacg agtatgggaa
     2941 acacctcacg cactgcatct tccggctccc ccctcctggc gaggacctgg ttcagttggc
     3001 aaaggcagct gtgagcggtg atgagaaagc cctggacatg tttaactgga agaagagcag
     3061 ttctggaagt ttgctgaaga tggggtctca cgaggggctg ttgttctttg tcagtctgat
     3121 ggacgggaca gtgcactatg tggatgagaa gggcaagacc actcaggtgg tgtccgcaga
     3181 cagcacgatt cagatgctgt tctacatgga gaagagggag gcactggtgg tggtcacaga
     3241 gaacctccgg ctgtccctgt acacggtgcc tcctgagggc aaagcagaag aagtgatgaa
     3301 ggtcaagctg agcgggaaaa ccggccgccg ggcagacatc gctttgattg aaggcagcct
     3361 tctcgtgatg gccgtcgggg aggctgccct cagattctgg gacatagaac gaggagagaa
     3421 ttatatactg agtccagatg agaagtttgg ctttgagaaa ggagagaata tgaactgtgt
     3481 gtgttactgt aaagtcaaag gtcttctggc cgctggtacc gacagagggc gagtagccat
     3541 gtggaggaaa gtaccagact tcctgggcag ccccggggca gagggcaagg acaggtgggc
     3601 ccttcagacc cctaccgagc tccaaggaaa catcacgcaa atccagtggg gttccaggaa
     3661 gaacctgctg gcagtgaaca gcgtcatctc cgtggccatc ctcagcgagc gggccatgtc
     3721 gtcacacttc caccagcaag tggccgccat gcaggtctcc ccgagtctgc tgaatgtgtg
     3781 cttcctgtcc acgggggtcg cacacagcct gcgcaccgac atgcacatca gtggagtgtt
     3841 tgccaccaag gatgctgtcg cagtctggaa cggaaggcag gtggcgatct tcgagctttc
     3901 tggagccgcg atacggagtg cagggacctt cttgtgtgag acgcctgtgt tagcaatgca
     3961 tgaagaaaac gtttacacgg tggagtcaaa ccgagttcaa gttcgaacct ggcaggggac
     4021 tgtcaaacaa ctcctccttt tctcggagac tgaggggaat ccctgcttct tggacatctg
     4081 tgggaatttc ctggttgtag ggacagactt ggctcacttt aaaagctttg atctttcccg
     4141 aagagaggcc aaagcacact gtagctgcag gagcctggcg gagctggtcc ctggggtggg
     4201 gggcatcgct tctctgcggt gcagcagcag cgggagcacc atcagcatcc tccccagcaa
     4261 ggctgacaac agccctgatt ccaaaatctg cttctacgat gttgaaatgg acacagtgac
     4321 cgtctttgac ttcaagactg gacaaattga tcggagagag acgctgtcct ttaatgagca
     4381 agagactaat aagagccacc tctttgtgga tgagggactg aaaaattatg ttcccgtgaa
     4441 ccacttctgg gaccagagtg agccccggct gtttgtatgc gaagccgtgc aggagacgcc
     4501 gcgctcccag cctcagtctg caaacgggca gccccaagat gggcgcgctg gccctgcggc
     4561 agatgttttg atcctgtcct tcttcatttc cgaagagcac ggcttcctgc ttcatgagag
     4621 cttcccccgg cctgccacct cccacagtct cctggggatg gaagtgcctt attactactt
     4681 cacaagaaag cccgaagaag cagacagaga agacgaggtg gagcctgggt gccaccacat
     4741 ccctcagatg gtgtccagga gacccctgcg agactttgtg gggctggagg actgcgacaa
     4801 ggccacccgg gacgccatgc tccacttcag cttctttgtc accataggag acatggacga
     4861 agccttcaaa tccatcaagc tcatcaaaag tgaggccgtc tgggagaaca tggcgcgcat
     4921 gtgcgtgaag acccagcggc tggacgtggc caaggtgtgc ctggggaaca tgggccatgc
     4981 ccgcggggcc cgagcgctgc gtgaggcgga gcaggagccg gagctagagg cccgcgtggc
     5041 cgtgctggcc acgcagctgg gcatgctgga ggacgccgag cagctgtaca ggaagtgcaa
     5101 gcgccacgac ctcctgaaca agttctacca ggctgcgggc cggtggcagg aggccctcca
     5161 ggtagccgag caccacgatc gcgtgcacct gcgcagcacc taccaccgct atgccgggca
     5221 cctggaggcc agcgccgact gcagccgggc cctcagttac tacgagaagt cggacacgca
     5281 ccgcttcgag gtgcccagga tgctgtcgga ggacctgccg tccctggagc tctacgtgaa
     5341 taaaatgaag gataagaccc tgtggcggtg gtgggcgcag tacctggaga gccagggcga
     5401 gatggacgcc gcgctgcact actacgagct ggcccgggac cacttctccc tggtccgcat
     5461 ccactgcttc cagggcaatg tccagaaggc tgcgcaaata gccaacgaga caggaaacct
     5521 ggcggcctcc taccacctcg cccgccagta cgagagccag gaggaggtcg ggcaggcggt
     5581 gcacttctac acccgggcac aggccttcaa gaatgccatc cgcctgtgca aggagaacgg
     5641 cctggacgac cagctcatga acttggccct gctgagctcc cccgaggaca tgatcgaggc
     5701 ggcccgatac tacgaggaga agggcgtgca gatggacagg gcggtcatgc tgtaccacaa
     5761 ggctggccac ttctccaagg ccctggagct ggcctttgcc acccagcagt ttgtggccct
     5821 acagctcata gcagaggacc tggatgagac gtcagaccct gcgctcctgg cccgctgctc
     5881 cgacttcttc atcgagcaca gtcagtacga gagggcggta gagctgctgc tggctgccag
     5941 gaagtatcag gaagccctgc agctgtgcct ggggcagaac atgagcatca ccgaggagat
     6001 ggcggaaaag atgaccgtgg ccaaggactc ctcggacctg cctgaggagt cgcggcggga
     6061 gctgctggag cagatagcag actgctgcat gcgccagggc agctaccacc tggccaccaa
     6121 gaagtacacg caggccggca acaagctgaa ggccatgagg gcgctgctca aatccggaga
     6181 cacggagaaa atcacgttct tcgcgagcgt gtccaggcag aaggaaatct acatcatggc
     6241 tgctaactac ctgcagtccc tggactggcg gaaggagccg gagatcatga agaacatcat
     6301 cggcttctac accaaggggc gggccctgga cctcctggct ggcttttatg acgcttgtgc
     6361 ccaggtggag attgatgaat accagaacta cgacaaagcc cacggggcgc tgaccgaggc
     6421 ctacaagtgc ctggccaagg ccaaggccaa gagccccctg gaccaggaga ccaggctggc
     6481 gcagctgcag agcaggatgg cactggtgaa gaggttcatc caggcccgca ggacgtacac
     6541 agaggacccc aaggagtcca tcaagcagtg tgagctgctc ctggaggaac cagacctgga
     6601 cagcaccatc cgcatcgggg acgtctatgg cttcctggtg gagcactacg tgcggaagga
     6661 ggaataccag acggcctaca gattcctgga ggagatgcgg cggcggcttc ccttggccaa
     6721 catgtcctac tacgtgagcc cgcaggccgt ggacgccgtg caccgggggc tgggtctccc
     6781 actgccacgc accgtccccg agcaggtccg ccacaacagc atggaggacg ccagggagct
     6841 ggacgaggag gtggtggaag aggcagatga cgacccctga gtcgacggta ccgcgggccc
     6901 gggatccacc ggatctagat aactgatcga ctcctcaggt gcaggctgcc tatcagaagg
     6961 tggtggctgg tgtggccaat gccctggctc acaaatacca ctgggatctt tttccctctg
     7021 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     7081 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     7141 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     7201 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     7261 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     7321 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     7381 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     7441 tcttatgaag atccctcgac ttaattaagg tacccaattc gccctatagt gagtcgtatt
     7501 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     7561 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     7621 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta
     7681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7861 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7921 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7981 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     8041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     8101 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8161 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8221 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     8281 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     8341 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     8401 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     8461 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8521 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8581 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8641 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8701 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8761 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8821 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8881 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8941 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9001 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9061 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9121 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9181 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9241 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9301 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9361 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9421 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9481 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9541 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9601 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9661 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9721 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9781 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9841 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9901 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9961 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10021 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    10081 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    10141 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    10201 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    10261 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
    10321 aaagctggag ctcatcgata c
//
