LOCUS       sequence-434238-        7629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for multivalent scaffold of 
            (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing 
            client proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Banani SF, Rice AM, Peeples WB, Lin Y, Jain S, Parker R, Rosen MK
  TITLE     Compositional Control of Phase-Separated Cellular Bodies.
  JOURNAL   Cell. 2016 Jul 28;166(3):651-663. doi: 10.1016/j.cell.2016.06.010. 
            Epub 2016 Jun 30.
  PUBMED    27374333
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.06.010"; journalName: "Cell"; date: 
            "2016-07-28- 28"; volume: "166"; issue: "3"; pages: "651-663"
COMMENT     Sequence Label: RFP-(SUMO)6-(SIM)10
FEATURES             Location/Qualifiers
     source          1..7629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1220..1927)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1297..1320)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1495..1514)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1764..1782
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1924..1933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1972..2175)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2001..2021)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2176..2479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2654..3242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2734..2753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3571..3618
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3643..3662
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(3850..4644)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(3850..4644)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3961..3980)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4571..4590
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4679..5036)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4693..4828
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4845..5036
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4993..5013
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(5038..5142)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5169..5624
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5256..5275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5466..5487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5631..5752
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5642..5661)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5696..5715
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tgtcttcatc ttccatctcc agttgtgccg gagtgtctgt ctcgttgatt ggctgaccat
       61 caaagcgaaa gcggatctgg cgcatcgaca gaccttgacg ctcacaatag gctttcatca
      121 gctttgacag cggggtgtgg cgtttaattt tgaactgcac cactgaacca tcttgacccg
      181 cgactttcag gttaatatga tcgttctcag tcttgacgcc ttctttgggt ttttcctcac
      241 tcatcgagcc accagaccca ccagagccac cggagccacc cactacagtt tgttgctgga
      301 agacgtcaat ggtgtcctcg tcttccatct ccagctgcgc cggtgtatcg gtttcgttga
      361 ttggctgacc atcaaagcga aaacgaattt gacgcatgct cagcccttga cgttcgcaat
      421 aggctttcat cagtttcgac agcggggtgt gacgtttgat tttaaactga acaacggaac
      481 cgtcctggcc cgccaccttc agattaatgt ggtcgttttc agtcttcaca ccctctttcg
      541 gtttttcttc ggacatagaa cccccgctac caccgctccc gccgcttccg ccaacaaccg
      601 tttgctgttg gaacacatcg atggtatctt catcttccat ttccagctgc gccggggtgt
      661 cggtttcgtt gattggctga ccatcaaagc gaaaacgaat ctggcgcatc gacaagcctt
      721 ggcgctcaca atacgccttc atcagcttac ttaatggggt atgacgttta atcttgaact
      781 gcacgactga gccgtcctga ccggcgacct tcaggttgat atggtcgttt tcggttttaa
      841 ctccttcttt tggtttctct tcgctcatgg aacctcccga ccccccgctt ccgccggagc
      901 cccctacgac ggtctgctgc tgaaatacgt cgatagtgtc ttcatcctcc atctccagct
      961 gtgccggcgt gtccgtttca ttaatcggtt gaccatcaaa gcggaagcga atctgacgca
     1021 tagaaagacc ctggcgttcg cagtacgctt tcatcagttt cgacaacggt gtgtggcgtt
     1081 taattttgaa ctgaaccacg gacccatcct gtccagcaac cttcaagtta atgtggtcat
     1141 tctcggtttt cacgccctcc ttaggcttct cttctgacat agaccctcca ctcccaccgc
     1201 tcccgccgga gcctccggac ttgtacagct cgtccatgcc gccggtggag tggcggccct
     1261 cggcgcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct
     1321 tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg taggtggtct
     1381 tgacctcggc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg atctcgccct
     1441 tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc atggtcttct
     1501 tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc ttgtagatga
     1561 actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg tcctcgaagt
     1621 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag tcggggatgt
     1681 cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac aggatgtccc
     1741 aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     1801 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gagccctcca
     1861 tgtgcacctt gaagcgcatg aactccttga tgatggccat gttgtcctcc tcgcccttgc
     1921 tcaccatggt ggcgaccggt agcgctagcg gatctgacgg ttcactaaac cagctctgct
     1981 tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt
     2041 acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat
     2101 ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg
     2161 ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg
     2221 aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac
     2281 gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta
     2341 ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac
     2401 atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt
     2461 taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat
     2521 tgattactat taataactaa tgcatggcgg taatacggtt atccacagaa tcaggggata
     2581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgat acagagttct
     3061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3361 aatgaagttt taaatcaatc taaagtatat atgagtaacc tgaggctatg gcagggcctg
     3421 ccgccccgac gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg
     3481 aaaaggaaga aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt
     3541 gccagccctg ggaccgaacc ccgcgtttat gaacaaacga cccaacaccg tgcgttttat
     3601 tctgtctttt tattgccgtc atagcgcggg ttccttccgg tattgtctcc ttccgtgttt
     3661 cagttagcct ccccctaggg tgggcgaaga actccagcat gagatccccg cgctggagga
     3721 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     3781 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     3841 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     3901 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     3961 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     4021 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     4081 atgggtcacg acgagatcct cgccgtcggg catgctcgcc ttgagcctgg cgaacagttc
     4141 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     4201 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     4261 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     4321 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     4381 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     4441 cgatagccgc gctgcctcgt cttgcagttc attcagggca ccggacaggt cggtcttgac
     4501 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     4561 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     4621 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcgatctt
     4681 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg
     4741 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     4801 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     4861 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     4921 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     4981 gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg cctcaggact
     5041 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5101 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5161 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5221 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5281 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5341 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5401 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5461 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5521 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5581 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttacgcct taagatacat
     5641 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     5701 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     5761 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     5821 gtaaaacctc tacaaatgtg gtatggctga ttatgatcag ttatctagat ccggtggatc
     5881 ccgggcccgc ggtaccgtcg acctaagatc cccccgagcc gccccgtttc gccggcgggt
     5941 cctcttcctc atcgctactg ctttcgatcg tcagatcgat aacgtccact ttagatccgc
     6001 cgctgccgcc gctgcccccg ctccctccgc gttttgctgg tggatcttct tcctcgtcgc
     6061 tactgctttc gatggtgagg tcaatcacgt caactttact gccgcctgaa ccgccagaac
     6121 cccctgagcc cccacgttta gccggtggat cttcttcttc atcgctgcta gattcgatcg
     6181 ttaagtcaat tacatcgact ttgcttccgc cgctgccgcc cgaacctccc gagccgccac
     6241 gtttcgctgg aggatcttcc tcttcatcgc tcgaagattc aattgtaagg tcaatgacat
     6301 caactttcga gccgccgctc ccgccagagc caccgctacc gccgcgtttg gcaggcggat
     6361 cttcttcttc gtcgctgctg ctttcgatgg tcaagtcgat cacatctact ttgctcccac
     6421 cagagccacc ggagcccccc gagccgcccc gtttcgccgg cgggtcctct tcctcatcgc
     6481 tactgctttc gatcgtcaga tcgataacgt ccactttaga tccgccgctg ccgccgctgc
     6541 ccccgctccc tccgcgtttt gctggtggat cttcttcctc gtcgctactg ctttcgatgg
     6601 tgaggtcaat cacgtcaact ttactgccgc ctgaaccgcc agaaccccct gagcccccac
     6661 gtttagccgg tggatcttct tcttcatcgc tgctagattc gatcgttaag tcaattacat
     6721 cgactttgct tccgccgctg ccgcccgaac ctcccgagcc gccacgtttc gctggaggat
     6781 cttcctcttc atcgctcgaa gattcaattg taaggtcaat gacatcaact ttcgagccgc
     6841 cgctcccgcc agagccaccg ctaccgccgc gtttggcagg cggatcttct tcttcgtcgc
     6901 tgctgctttc gatggtcaag tcgatcacat ctactttgct cccaccagag ccacctgatc
     6961 caccggagcc gccgaccact gtctgttgct gaaacacgtc aatagtatcc tcgtcttcca
     7021 tttccagttg tgccggggtg tccgtttcat taattggttg cccgtcaaag cgaaagcgaa
     7081 tctggcgcat agacaggcct tggcgttcgc aatatgcttt catcagttta gataacgggg
     7141 tgtgacgctt gattttaaat tgcactacac taccatcttg gcccgcaact ttcagattaa
     7201 tgtgatcatt ttcggtcttt accccttcct tcggcttttc ctcagacata ctaccacctg
     7261 atccccctga tccaccggag ccgccgacca ctgtctgttg ctgaaacacg tcaatagtat
     7321 cctcgtcttc catttccagt tgtgccgggg tgtccgtttc attaattggt tgcccgtcaa
     7381 agcgaaagcg aatctggcgc atagacaggc cttggcgttc gcaatatgct ttcatcagtt
     7441 tagataacgg ggtgtgacgc ttgattttaa attgcactac actaccatct tggcccgcaa
     7501 ctttcagatt aatgtgatca ttttcggtct ttaccccttc cttcggcttt tcctcagaca
     7561 tactaccacc tgatcccccg ctaccgccgc tccctcccac cacggtctgc tgttgaaaaa
     7621 catcgatgg
//
