LOCUS       sequence-43427-e         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Rhee JM, Pirity MK, Lackan CS, Long JZ, Kondoh G, Takeda J, 
            Hadjantonakis AK
  TITLE     In vivo imaging and differential localization of lipid-modified 
            GFP-variant fusions in embryonic stem cells and mice.
  JOURNAL   Genesis. 2006 Apr;44(4):202-18.
  PUBMED    16604528
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis."; 
            date: "2006-04"; volume: "44(4)"; pages: "202-18"
COMMENT     Sequence Label: pCAG:myr-Venus
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..40
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             join(47..49,50..763)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(89..110)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(350..369)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     697..718
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(849..868)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 ttttggcaaa ttcgccacca tgggaagcag caagagcaag ccaaaggtga gcaagggcga
       61 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      121 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      181 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
      241 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
      301 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
      361 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
      421 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
      481 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
      541 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
      601 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
      661 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
      721 cgccgccggg atcactctcg gcatggacga gctgtacaag taagaattca ctcctcaggt
      781 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
      841 actgaagatc tttttccctc tgccaaaaat tatgggggac atcatgaagc cccnttgagc
      901 atgtgacttc tggctaataa tggaaattta tttttcattg caattagtgt gttggnaatt
      961 tttttgtgtc tctcacttcg gga
//
