LOCUS       sequence-43428-e        9528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, 
            Lee TS
  TITLE     Identification and microbial production of a terpene-based advanced 
            biofuel.
  JOURNAL   Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494.
  PUBMED    21952217
REFERENCE   2  (bases 1 to 9528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-09-27"; volume: "2:483. doi"; pages: " 
            10.1038/ncomms1494"
COMMENT     Sequence Label: pRSLeu2d-BISopt
FEATURES             Location/Qualifiers
     source          1..9528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(26..467)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(32..55)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    350..467
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(491..511)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(491..509)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(530..546)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(530..546)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(543..565)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    554..570
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(578..584,585..602,603..608))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(761..778)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(932..1520)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1012..1031)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1691..2482,2483..2551))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2314..2333
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2552..2656)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2683..4025
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(3646..3693)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     4066..4084
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4122..4144)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4244..4263
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(4756..5850)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVAGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANLLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      complement(6157..6612)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6294..6315)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6506..6525
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6742..6764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6756..6773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6757..6773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6783..6802
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6783..6801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(6820..7067)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     7033..7051
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 ctagttctag aatccggggt tttttctcct tgacgttaaa gtatagaggt atattaacaa
       61 ttttttgttg atacttttat tacatttgaa taagaagtaa tacaaaccga aaatgttgaa
      121 agtattagtt aaagtggtta tgcagttttt gcatttatat atctgttaat agatcaaaaa
      181 tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc gcattatcat
      241 cctatggttg ttaatttgat tcgttcattt gaaggtttgt ggggccaggt tactgccaat
      301 ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc ggagcagtgc
      361 ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agacgaggac gcacggagga
      421 gagtcttcct tcggagggct gtcacccgct cggcggcttc taatccgtac tagagctcca
      481 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
      541 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc ggaagcataa
      601 agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg ttgcgctcac
      661 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      721 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      781 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
      841 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
      901 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      961 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1021 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1081 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1141 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1201 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1261 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1321 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     1381 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1441 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1501 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1561 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1621 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1681 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1741 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1801 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1861 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1921 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1981 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2041 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2101 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2161 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2221 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2281 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2341 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2401 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2461 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2521 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2581 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2641 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt
     2701 cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     2761 tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt
     2821 gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc
     2881 tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa
     2941 tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag
     3001 catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt
     3061 ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca
     3121 taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat
     3181 tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt
     3241 gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc
     3301 ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga
     3361 ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa
     3421 acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt
     3481 aggttatata gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt
     3541 gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt
     3601 ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt
     3661 tctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     3721 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     3781 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     3841 cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc ctgtgatatt
     3901 atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag ctgttctata
     3961 tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc ctttgatatt
     4021 ggatcatact aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     4081 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     4141 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     4201 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     4261 gtactgagag tgcaccatat cgactacgtc gtaaggccgt ttctgacaga gtaaaattct
     4321 tgagggaact ttcaccatta tgggaaatgc ttcaagaagg tattgactta aactccatca
     4381 aatggtcagg tcattgagtg ttttttattt gttgtatttt ttttttttta gagaaaatcc
     4441 tccaatatca aattaggaat cgtagtttca tgattttctg ttacacctaa ctttttgtgt
     4501 ggtgccctcc tccttgtcaa tattaatgtt aaagtgcaat tctttttcct tatcacgttg
     4561 agccattagt atcaatttgc ttacctgtat tcctttacta tcctcctttt tctccttctt
     4621 gataaatgta tgtagattgc gtatatagtt tcgtctaccc tatgaacata ttccattttg
     4681 taatttcgtg tcgtttctat tatgaatttc atttataaag tttatgtaca aatatcataa
     4741 aaaaagagaa tctttttaag caaggatttt cttaacttct tcggcgacag catcaccgac
     4801 ttcggtggta ctgttggaac cacctaaatc accagttctg atacctgcat ccaaaacctt
     4861 tttaactgca tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca atttcaacat
     4921 cattgcagca gacaagatag tggcgatagg gtcaacctta ttctttggca aatctggagc
     4981 agaaccgtgg catggttcgt acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa
     5041 ggacgcagat ggcaacaaac ccaaggaacc tgggataacg gaggcttcat cggagatgat
     5101 atcaccaaac atgttgctgg tgattataat accatttagg tgggttgggt tcttaactag
     5161 gatcatggcg gcagaatcaa tcaattgatg ttgaaccttc aatgtaggga attcgttctt
     5221 gatggtttcc tccacagttt ttctccataa tcttgaagag gccaaaagat tagctttatc
     5281 caaggaccaa ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt
     5341 gattctttgc acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc
     5401 gtcttccttt ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc
     5461 agtaccttta gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa
     5521 gttacatggt cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg
     5581 ttcaggtcta acactaccgg taccccattt aggaccagcc acagcaccta acaaaacggc
     5641 atcaaccttc ttggaggctt ccagcgcctc atctggaagt gggacacctg tagcatcgat
     5701 agcagcacca ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga
     5761 aatagcttta agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg
     5821 caaaacgacg atcttcttag gggcagacat tacaatggta tatccttgaa atatatataa
     5881 aaaaaggcgc cttagaccgc tcggccaaac aaccaattac ttgttgagaa atagagtata
     5941 attatcctat aaatataacg tttttgaaca cacatgaaca aggaagtaca ggacaattga
     6001 ttttgaagag aatgtggatt ttgatgtaat tgttgggatt ccatttttaa taaggcaata
     6061 atattaggta tgtggatata ctagaagttc tcctcgaccg tcgatatgcg gtgtgaaata
     6121 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt
     6181 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     6241 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     6301 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6361 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     6421 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6481 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6541 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6601 tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6661 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     6721 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     6781 cgtaatacga ctcactatag ggcgaattgg gtaccggccg caaattaaag ccttcgagcg
     6841 tcccaaaacc ttctcaagca aggttttcag tataatgtta catgcgtaca cgcgtctgta
     6901 cagaaaaaaa agaaaaattt gaaatataaa taacgttctt aatactaaca taactataaa
     6961 aaaataaata gggacctaga cttcaggttg tctaactcct tccttttcgg ttagagcgga
     7021 tgtgggggga gggcgtgaat gtaagcgtga cataactaat tacatgactc gagttacagc
     7081 ggcagcggtt cgatcaggca ttctttgatg tggtctttaa cttccagttt agaaacaccg
     7141 aaaccgtcac cgtctttgaa gataaccata gtaacacggg tttcttcgaa cagcattttt
     7201 ttgcacgcga acggaacgtc gtccggtttc aggaactcac gggtcagttc tttaaccgcc
     7261 ggttccagga tagagtagat gtggtccagc gcttcttctt cggtgcattc cgggtggtct
     7321 ttcatgtagc attcgataga agacgccagt tcaccacgcg ctttttccgc tttgtaggtt
     7381 ttggtgtcgt ccgccagacg agagatcaga gagttcagtt cggtcagaac acgacgaccc
     7441 ggatagtcaa ctttttccag cgcttcctga gacagcagct gaccgtccat gatcagaaca
     7501 ccagacagca gcaggatacg ctgaccgata gaggtgatac cgtttttgat gtattcgtcc
     7561 agggtcggaa cgtattccgc cgccagccat tccgcttctt cgtagtaacc cagcaggtag
     7621 tcttcccaac ctttacggaa gaaagaaacc agttcacgac cctgttcttt ttccgcttcc
     7681 cacgcaactt cgtgaacgat gtcgtagtaa atctggtagc acagtttcat gtagtccggc
     7741 aggttttcgg tgaaagacag gtcccaacga cgaaccgctt cggtgaacag tttcagttcg
     7801 tccagggtac cgtaggtgtc gtacatgtcg tccagaacgg tctgcaggca cgcaactttg
     7861 gtgaacagga aacggcattt cgcgtactgc ggttcgtagg tacccgccgc aaccaggaag
     7921 tagaactcca gcggacgttc acgcgcgaag gtgaacagcg gcagaccaga gtcacggaac
     7981 caagaggtaa cgtttttcat ttcttcctgg tggatagact ggatgatgtt gaagtccagt
     8041 ttacccagtt ccaggaattt ttcgttgttc acgtacggca gtttgtaaac gcatttcgcg
     8101 atacgcgcgt agtccggacg gtaaacctgg cagtaacgtt tcgcttcaac acgcggaaca
     8161 gacgcgtgcc aagaggtttt cagcgcgtat ttgatttcct gagacaggtt ctgtttgttg
     8221 ttccacgcag aagaggtttc agatttttcc agcgcttcac gcaggtattt ggtcgcgaaa
     8281 gatttcgctt cgtccaggat gttttcaccc ggaaacgcca gctgagacgc acggtacagg
     8341 ttcagcatag acgcaacgtc tttgttgaac tgaccggtag agcagatgaa tttaccgttc
     8401 gcgtctttga agttgtcgaa gatcgccgga gaaacgttgt aacggtgcag acgcagcaga
     8461 cggaaaccca gcgcggtggt ttccaggtcc gcgatcgggt tcagacgacc ccaaccgata
     8521 ccacgttcgt tccagtgacg gtaaacgtag tccagcgctt ctttgatttc tttttcgaag
     8581 tgacggtaga tacccagacg aacgatgttg tcaacgatca gcagacgttc cagcaggtca
     8641 accgggtaca ggcacggaac gaagttaccg aatttgatca taacagagtt caggaactcc
     8701 aggcatttcg cgtcaccggt gtgcatgaaa acgcacgcgg tagacgccgg agaagacagg
     8761 aaagaaccgt cctgagactg aacttccatg atacgttccc attcaacgat gtcctggata
     8821 ccttccagag agtacagcag cggagacgga accgcgtaga tttcttcacg agacagtttc
     8881 gccagacgtt cctgacgttt ggtctgcagc aggtggatgt acggcaggtc gtacggcaga
     8941 cccagacgca gagactgcgc ttcacgcagc agagacggga agatgatttc gaagtcggta
     9001 accagagagt cctggtcggt ttcgtcttta accagttcca ggttagattt gatgaactcg
     9061 ataccctgtt caacctgcag gtcaccaacg ttccatttca gcagaaccag aacgcaagac
     9121 agggtcgcca gcagacggtc agacagcagg aagtgagact ggatacccca agaaccgtct
     9181 ttcagctggt ttttcagaat ccagtcaacg gtctgcggga actgcggacg cgcagaaccg
     9241 tcgatcgccg gaacacgcgc aacccacgcg gtgtcgtacg cagacggggt gatcatagat
     9301 tcaccgtcac cggtgatcgc cgggttcagc atcgctttga tttcagaaac cagaacttcc
     9361 gcacgttcac ggtaagaaga gtcgatgtac ggagatttca gagagtgaac caggtcgtaa
     9421 ccccaaacgt tcgggtgcgg gttcgcggta cgacggatca gaccagaggt agaagacagg
     9481 tcgcaaacca gagaagaaac tttagaaacc gcagaaacac ccgccatg
//
