LOCUS       sequence-43429-e       11022 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11022)
  AUTHORS   Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, 
            Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, 
            Ishitani R, Deisseroth K, Nureki O
  TITLE     Crystal structure of the channelrhodopsin light-gated cation 
            channel.
  JOURNAL   Nature. 2012 Jan 22. doi: 10.1038/nature10870.
  PUBMED    22266941
REFERENCE   2  (bases 1 to 11022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2012 Jan 22. doi: 10.1038/nature10870."
COMMENT     Sequence Label: pLenti-CaMKIIa-C1C2-EYFP
FEATURES             Location/Qualifiers
     source          1..11022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3890
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             join(4973..4975,4976..5689)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5015..5036)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5276..5295)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5623..5644
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5714..6302
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5767..5787)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6185..6196)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6305..6321)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6306..6322)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6827..7007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7033..7050)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7039..7263
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7309..7737
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7396..7415)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7606..7627
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7746..7766)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7751..8080
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7931..8066
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        8128..8175
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8194..8568
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8698..8819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8735..8754)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8789..8808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8868..8884)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8868..8884)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8881..8903)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8892..8908
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8916..8922,8923..8940,8941..8946))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8961..8982
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9099..9116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9270..9858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9350..9369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10029..10820,10821..10889))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10652..10671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10890..10994)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acattatggc cttaggtcac ttcatctcca tggggttctt
     2641 cttctgattt tctagaaaat gagatggggg tgcagagagc ttcctcagtg acctgcccag
     2701 ggtcacatca gaaatgtcag agctagaact tgaactcaga ttactaatct taaattccat
     2761 gccttggggg catgcaagta cgatatacag aaggagtgaa ctcattaggg cagatgacca
     2821 atgagtttag gaaagaagag tccagggcag ggtacatcta caccacccgc ccagccctgg
     2881 gtgagtccag ccacgttcac ctcattatag ttgcctctct ccagtcctac cttgacggga
     2941 agcacaagca gaaactggga caggagcccc aggagaccaa atcttcatgg tccctctggg
     3001 aggatgggtg gggagagctg tggcagaggc ctcaggaggg gccctgctgc tcagtggtga
     3061 cagatagggg tgagaaagca gacagagtca ttccgtcagc attctgggtc tgtttggtac
     3121 ttcttctcac gctaaggtgg cggtgtgata tgcacaatgg ctaaaaagca gggagagctg
     3181 gaaagaaaca aggacagaga cagaggccaa gtcaaccaga ccaattccca gaggaagcaa
     3241 agaaaccatt acagagacta caagggggaa gggaaggaga gatgaattag cttcccctgt
     3301 aaaccttaga acccagctgt tgccagggca acggggcaat acctgtctct tcagaggaga
     3361 tgaagttgcc agggtaacta catcctgtct ttctcaagga ccatcccaga atgtggcacc
     3421 cactagccgt taccatagca actgcctctt tgccccactt aatcccatcc cgtctgttaa
     3481 aagggcccta tagttggagg tgggggaggt aggaagagcg atgatcactt gtggactaag
     3541 tttgttcgca tccccttctc caaccccctc agtacatcac cctgggggaa cagggtccac
     3601 ttgctcctgg gcccacacag tcctgcagta ttgtgtatat aaggccaggg caaagaggag
     3661 caggttttaa agtgaaaggc aggcaggtgt tggggaggca gttaccgggg caacgggaac
     3721 agggcgtttc ggaggtggtt gccatgggga cctggatgct gacgaaggct cgcgaggctg
     3781 tgagcagcca cagtgccctg ctcagaagcc ccaagctcgt cagtcaagcc ggttctccgt
     3841 ttgcactcag gagcacgggc aggcgagtgg cccctagttc tgggggcagc gggggatccc
     3901 cgggtaccgg taggccacca tgtcgcggag gccatggctt cttgccctag cgctggcagt
     3961 ggcgctggcg gccggcagcg caggagcctc gactggcagt gacgcgacgg tgccggtcgc
     4021 gactcaggat ggccccgact acgttttcca ccgtgcccac gagcgcatgc tcttccaaac
     4081 ctcatacact cttgagaaca atggttctgt tatttgcatc ccgaacaacg gccagtgctt
     4141 ctgcttggct tggcttaaat ccaacggaac aaatgccgag aagttggctg ccaacattct
     4201 gcagtggatt acttttgcgc tttcagcgct ctgcctgatg ttctacggct accagacctg
     4261 gaagtctact tgcggctggg aggagattta cgtggccacg atcgagatga tcaagttcat
     4321 catcgagtat ttccatgagt ttgacgaacc tgcggtgatc tactcatcca acggcaacaa
     4381 gaccgtgtgg cttcgttacg cggagtggct gctgacctgc cctgtcattc ttatccatct
     4441 gagcaacctt acgggtctgg cgaacgacta taacaagcgt accatgggtc tgctggtgtc
     4501 agatatcggc acgatcgtgt ggggcaccac ggccgcgctg tccaagggat acgtccgtgt
     4561 cattttcttc ctgatgggcc tgtgctacgg catctacaca ttcttcaacg cagccaaggt
     4621 ctacattgag gcgtaccaca ccgtgccgaa gggccggtgt cgccaggtgg tgactggcat
     4681 ggcttggctc ttcttcgtat catggggtat gttccccatc ctgttcatcc tcggccccga
     4741 gggcttcggc gtcctgagcg tgtacggctc caccgtcggc cacaccatca ttgacctgat
     4801 gtcgaagaac tgctggggtc tgctcggcca ctacctgcgc gtgctgatcc acgagcatat
     4861 cctcatccac ggcgacattc gcaagaccac caaattgaac attggtggca ctgagattga
     4921 ggtcgagacg ctggtggagg acgaggccga ggctggcgcg gtcgcggccg ccgtgagcaa
     4981 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     5041 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     5101 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     5161 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     5221 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     5281 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     5341 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     5401 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     5461 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     5521 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     5581 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     5641 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtgag aattcgatat
     5701 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5761 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5821 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5881 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5941 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6001 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6061 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     6121 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     6181 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     6241 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     6301 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     6361 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     6421 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6481 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6541 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     6601 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     6661 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     6721 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     6781 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     6841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     7021 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     7081 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     7141 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     7201 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     7261 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     7321 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7381 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7441 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7501 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7561 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7621 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7681 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     7741 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7801 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7861 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7921 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7981 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     8041 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     8101 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     8161 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     8221 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     8281 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     8341 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     8401 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     8461 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     8521 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     8581 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8641 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8821 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8881 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8941 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     9001 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9061 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     9121 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     9181 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     9241 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     9301 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     9361 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     9421 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9481 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9541 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9601 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9661 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9721 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9781 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9841 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9901 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9961 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    10021 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    10081 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    10141 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    10201 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    10261 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    10321 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    10381 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    10441 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10501 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10561 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10621 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10681 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10741 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10801 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10861 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10921 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10981 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
