LOCUS       sequence-434349-       10171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector with XylS-Pm expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ
  TITLE     A series of vectors for inducible gene expression in 
            multidrug-resistant Acinetobacter baumannii.
  JOURNAL   Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 
            10.1128/aem.00474-24. Epub 2024 Aug 20.
  PUBMED    39162403
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2024-09-18"; volume: "90(9):e0047424. 
            doi"; pages: " 10.1128/aem.00474-24. Epub 2024 Aug 20"
COMMENT     Sequence Label: pMApra-Ptol
FEATURES             Location/Qualifiers
     source          1..10171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..840
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             827..1678
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1954..2919)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     terminator      4025..4111
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4203..4230
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4249..4340
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4783..4885
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4952..4971)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5298..6098
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      6628..7022
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            7364..7451
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8690..9661
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taatcgttcg taatcaagcc acttcctttt tgcattgacg
     1981 cagggtgtcg gaaggcaact cgccgaacgc gctcctatag ttttcagcga agcgtcccaa
     2041 atgtaagaag ccgtagtcta gggctatctc agttatacta cgcacattgg cactgggatc
     2101 gttcaagcag gcgcggatgc tttcgagctt gcggttgcgg atgtagttct tcggcgtggt
     2161 gccggcatgc ttctcgaaca aattgtagag cgagcgtgga ctcatcatcg ccagctccgc
     2221 taaccgctca aggctgatat tccgtttgag attctcctca atgaattgaa cgactcgctc
     2281 gaaagacggg ttacctttgc tgaaaatttc acggctgaca ttgctgccca gcatttcgag
     2341 cagcttggaa gcgatgatcc ccgcatagtg ctcttggacc cgaggcatcg actttgtatg
     2401 ttccgcttcg tcacaaacta acccgagtag attgataaag ccatcgagtt gctggagatt
     2461 gtgtcgcgcg gcgaaacgga taccctccct cggcttgtgc caattgttgt cactgcatgc
     2521 ccgatcaagg accactgagg gcaatttaac gataaatttc tcgcaatctt ctgaataggt
     2581 caggtcggct tggtcatccg gattgagcag caatagttcg cccggcgcaa aatagtgctc
     2641 ctggccatgg ccacgccaca ggcaatggcc tttgagtatt atttgcagat gataacaggt
     2701 ctctaatcca ggcgagatta ccctcacgct accgccgtag ctgattcgac acaggtcgag
     2761 gcatccgaag attctgtggt gcagcctgcc tgccgggcgc ccgcccttgg gcaggcgaat
     2821 agagtgcgta ccgacatact ggttaacata atcggagact gcatagggct cggcgtggac
     2881 gaagatctga cttttctcgt tcaataagca aaaatccata gttcacggtt ctcttatttt
     2941 aatgtgggct gcttggtgtg atgtagaaag gcgccaagtc gatgaaaatg catctcgacg
     3001 tgatgcgtat acgggttacc cccattgcca cgttgcgcca tcctttttgc aatcagtgac
     3061 cacttttcca agcaaaaata acgccaagca gaacgaagac gttcttttta agaagcgaga
     3121 acaccagaag ttcgtgctgt cggggcatgc ggcgacgaat tggcggataa aggggatctg
     3181 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     3241 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattacgtat ggcaatgaaa
     3301 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     3361 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     3421 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     3481 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     3541 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgcatgg gaattagctt
     3601 gatctgacca acgaccggta gcggagctat ccaacggcgg tataccagga aaacacacag
     3661 caggtacatc agaacagtac catgactgaa gaacaaatag ttttttcctg atccataaag
     3721 cagaacggcc tgctccatga caaatctggc tccccaacta atgccccatg cagccagcat
     3781 aaccagcata aactgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca
     3841 ggagtgcaaa aaatggctat ctctagaaag gcctacccct taggctggat ccacgtcgaa
     3901 ttcctgcaga agcttactag tggctgaatc ccgaaacagt tcgcaggtaa tagttagagc
     3961 ctgcataacg gtttcgggat tttttatagg tctccccatg cgagagtagg gaactgccag
     4021 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     4081 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     4141 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     4201 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     4261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4501 tccttgagag ttttcgcccc gaagaacgtg atgtccggcg gtgcttttgc cgttacgcac
     4561 caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca ctggcctcaa
     4621 aaacaccatc atacactaaa tcagtaagtt ggcagcatca cccgacgcac tttgcgccga
     4681 ataaatacct gtgacggaag atcacttcgc agaataaata aatcctggtg tccctgttga
     4741 taccgggaag ccctgggcca acttttggcg aaaatgagac gttgatcggc acgtaagagg
     4801 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
     4861 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     4921 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     4981 ctataaccag accgttcagc tggatattac ggccttttta tcaaggcccg atccttggag
     5041 cccttgccct cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt
     5101 tgcaaaccct cactgatccg gctcacggta actgatgccg tatttgcagt accagcgtac
     5161 ggcccacaga atgatgtcac gctgaaaatg ccggcctttg aatgggttca tgtgcagctc
     5221 catcagcaaa aggggatgat aagtttatca ccacctacta tttgcaacag tgccgttgat
     5281 cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa tgtcgtgcaa tacgaatggc
     5341 gaaaagccga gctcatcggt cagcttctca accttggggt tacccccggc ggtgtgctgc
     5401 tggtccacag ctccttccgt agcgtccggc ccctcgaaga tgggccactt ggactgatcg
     5461 aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt catgccctcg tggtcaggtc
     5521 tggacgacga gccgttcgat cctgccacgt cgcccgttac accggacctt ggagttgtct
     5581 ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc ccatccattt gcctttgcgg
     5641 cagcggggcc acaggcagag cagatcatct ctgatccatt gcccctgcca cctcactcgc
     5701 ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca ggtacttctc ctcggcgtgg
     5761 gacacgatgc caacacgacg ctgcatcttg ccgagttgat ggcaaaggtt ccctatgggg
     5821 tgccgagaca ctgcaccatt cttcaggatg gcaagttggt acgcgtcgat tatctcgaga
     5881 atgaccactg ctgtgagcgc tttgccttgg cggacaggtg gctcaaggag aagagccttc
     5941 agaaggaagg tccagtcggt catgcctttg ctcggttgat ccgctcccgc gacattgtgg
     6001 cgacagccct gggtcaactg ggccgagatc cgttgatctt cctgcatccg ccagaggcgg
     6061 gatgcgaaga atgcgatgcc gctcgccagt cgattggctg agctcatgaa aacgtggcca
     6121 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     6181 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     6241 gcagaatgct taatgaatta caacagtact gcgatgagtg gcagggcggg gcgtaatttt
     6301 tttaaggcag ttattgtctt caaattcccg ttgcacatag cccggcaatt cctttccctg
     6361 ctctgccata agcgcagcga atgccgggta atactcgtca acgatctgat agagaagggt
     6421 ttgctcgggt cggtggctct ggtaacgacc agtatcccga tcccggctgg ccgtcctggc
     6481 cgccacatga ggcatgttcc gcgtccttgc aatactgtgt ttacatacag tctatcgctt
     6541 agcggaaagt tcttttaccc tcagccgaaa tgcctgccgt tgctagacat tgccagccag
     6601 tgcccgtcac tcccgtacta actgtcacga acccctgcaa taactgtcac gcccccctgc
     6661 aataactgtc acgaacccct gcaataactg tcacgccccc aaacctgcaa acccagcagg
     6721 ggcgggggct ggcggggtgt tggaaaaatc catccatgat tatctaagaa taatccacta
     6781 ggcgcggtta tcagcgccct tgtggggcgc tgctgccctt gcccaatatg cccggccaga
     6841 ggccggatag ctggtctatt cgctgcgcta ggctacacac cgccccaccg ctgcgcggca
     6901 gggggaaagg cgggcaaagc ccgctaaacc ccacaccaaa ccccgcagaa atacgctgga
     6961 gcgcttttag ccgctttagc ggcctttccc cctacccgaa gggtggggcc gcgtgtgcag
     7021 ccccgcaggg cctgtctcgg tcgatcattc agcccggctc atccttctgg cgtggcggca
     7081 gaccgaacaa ggcgcggtcg tggtcgcgtt caaggtacgc atccattgcc gccatgagcc
     7141 gatcctccgg ccactcgctg ctgttcacct tggccaaaat catggccccc accagcacct
     7201 tgcgccttgt ttcgttcttg cgctcttgct gctgttccct tgcccgcacc cgctgaattt
     7261 cggcattgat tcgcgctcgt tgttcttcga gcttggccag ccgatccgcc gccttgttgc
     7321 tccccttaac catcttgaca ccccattgtt aatgtgctgt ctcgtaggct atcatggagg
     7381 cacagcggcg gcaatcccga ccctactttg taggggaggg cgcacttacc ggtttctctt
     7441 cgagaaactg gcctaacggc cacccttcgg gcggtgcgct ctccgagggc cattgcatgg
     7501 agccgaaaag caaaagcaac agcgaggcag catggcgatt tatcacctta cggcgaaaac
     7561 cggcagcagg tcgggcggcc aatcggccag ggccaaggcc gactacatcc agcgcgaagg
     7621 caagtatgcc cgcgacatgg atgaagtctt gcacgccgaa tccgggcaca tgccggagtt
     7681 cgtcgagcgg cccgccgact actgggatgc tgccgacctg tatgaacgcg ccaatgggcg
     7741 gctgttcaag gaggtcgaat ttgccctgcc ggtcgagctg accctcgacc agcagaaggc
     7801 gctggcgtcc gagttcgccc agcacctgac cggtgccgag cgcctgccgt atacgctggc
     7861 catccatgcc ggtggcggcg agaacccgca ctgccacctg atgatctccg agcggatcaa
     7921 tgacggcatc gagcggcccg ccgctcagtg gttcaagcgg tacaacggca agaccccgga
     7981 gaagggcggg gcacagaaga ccgaagcgct caagcccaag gcatggcttg agcagacccg
     8041 cgaggcatgg gccgaccatg ccaaccgggc attagagcgg gctggccacg acgcccgcat
     8101 tgaccacaga acacttgagg cgcagggcat cgagcgcctg cccggtgttc acctggggcc
     8161 gaacgtggtg gagatggaag gccggggcat ccgcaccgac cgggcagacg tggccctgaa
     8221 catcgacacc gccaacgccc agatcatcga cttacaggaa taccgggagg caatagacca
     8281 tgaacgcaat cgacagagtg aagaaatcca gaggcatcaa cgagttagcg gagcagatcg
     8341 aaccgctggc ccagagcatg gcgacactgg ccgacgaagc ccggcaggtc atgagccaga
     8401 cccagcaggc cagcgaggcg caggcggcgg agtggctgaa agcccagcgc cagacagggg
     8461 cggcatgggt ggagctggcc aaagagttgc gggaggtagc cgccgaggtg agcagcgccg
     8521 cgcagagcgc ccggagcgcg tcgcgggggt ggcactggaa gctatggcta accgtgatgc
     8581 tggcttccat gatgcctacg gtggtgctgc tgatcgcatc gttgctcttg ctcgacctga
     8641 cgccactgac aaccgaggac ggctcgatct ggctgcgctt ggtggcccga tgaagaacga
     8701 caggactttg caggccatag gccgacagct caaggccatg ggctgtgagc gcttcgatat
     8761 cggcgtcagg gacgccacca ccggccagat gatgaaccgg gaatggtcag ccgccgaagt
     8821 gctccagaac acgccatggc tcaagcggat gaatgcccag ggcaatgacg tgtatatcag
     8881 gcccgccgag caggagcggc atggtctggt gctggtggac gacctcagcg agtttgacct
     8941 ggatgacatg aaagccgagg gccgggagcc tgccctggta gtggaaacca gcccgaagaa
     9001 ctatcaggca tgggtcaagg tggccgacgc cgcaggcggt gaacttcggg ggcagattgc
     9061 ccggacgctg gccagcgagt acgacgccga cccggccagc gccgacagcc gccactatgg
     9121 ccgcttggcg ggcttcacca accgcaagga caagcacacc acccgcgccg gttatcagcc
     9181 gtgggtgctg ctgcgtgaat ccaagggcaa gaccgccacc gctggcccgg cgctggtgca
     9241 gcaggctggc cagcagatcg agcaggccca gcggcagcag gagaaggccc gcaggctggc
     9301 cagcctcgaa ctgcccgagc ggcagcttag ccgccaccgg cgcacggcgc tggacgagta
     9361 ccgcagcgag atggccgggc tggtcaagcg cttcggtgat gacctcagca agtgcgactt
     9421 tatcgccgcg cagaagctgg ccagccgggg ccgcagtgcc gaggaaatcg gcaaggccat
     9481 ggccgaggcc agcccagcgc tggcagagcg caagcccggc cacgaagcgg attacatcga
     9541 gcgcaccgtc agcaaggtca tgggtctgcc cagcgtccag cttgcgcggg ccgagctggc
     9601 acgggcaccg gcaccccgcc agcgaggcat ggacaggggc gggccagatt tcagcatgta
     9661 gtgcttgcgt tggtactcac gcctgttata ctatgagtac tcacgcacag aagggggttt
     9721 tatggaatac gaaaaaagcg cttcagggtc ggtctacctg atcaaaagtg acaagggcta
     9781 ttggttgccc ggtggctttg gttatacgtc aaacaaggcc gaggctggcc gcttttcagt
     9841 cgctgatatg gccagcctta accttgacgg ctgcaccttg tccttgttcc gcgaagacaa
     9901 gcctttcggc cccggcaagt ttctcggtga ctgatatgaa agaccaaaag gacaagcaga
     9961 ccggcgacct gctggccagc cctgacgctg tacgccaagc gcgatatgcc gagcgcatga
    10021 aggccaaagg gatgcgtcag cgcaagttct ggctgaccga cgacgaatac gaggcgctgc
    10081 gcgagtgcct ggaagaactc agagcggcgc agggcggggg tagtgacccc gccagcgcct
    10141 aaccaccaac tgcctgcaaa ggaggcaatc a
//
