LOCUS       sequence-434354-        8548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally EGFP-tagged IFT74 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Nishijima Y, Hagiya Y, Kubo T, Takei R, Katoh Y, Nakayama K
  TITLE     RABL2 interacts with the intraflagellar transport-B complex and 
            CEP19 and participates in ciliary assembly.
  JOURNAL   Mol Biol Cell. 2017 Jun 15;28(12):1652-1666. doi: 
            10.1091/mbc.E17-01-0017. Epub 2017 Apr 20.
  PUBMED    28428259
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-01-0017"; journalName: "Mol Biol Cell"; date: 
            "2017-06-15- 15"; volume: "28"; issue: "12"; pages: "1652-1666"
COMMENT     Sequence Label: pCAG-EGFP-C-IFT74
FEATURES             Location/Qualifiers
     source          1..8548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1743..1752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1749..1751,1752..1754,1755..2465)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1749..1751,1752..1754,1755..2465)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1794..1815)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2055..2074)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2402..2423
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4420..4439)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4485..4540
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4539..4558)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4901..4917)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4901..4917)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4914..4936)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4925..4941
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4949..4955,4956..4973,4974..4979))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4994..5015
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5074..5269
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5120..5255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5275..5409
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5312..5331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5366..5385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5477..5494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5648..6236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5728..5747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6407..7198,7199..7267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7030..7049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7268..7372)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7684..8487
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7747..7766)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8357..8376
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8494..8542
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat ttgctagttt
     1741 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1801 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1861 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1921 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1981 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     2041 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2101 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2161 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2221 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2281 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2341 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2401 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2461 acaagtccgg actcagatct atggccagca atcacaaatc ttcagcagct cgccctgttt
     2521 caagaggtgg agttgggtta acaggaaggc ctccttctgg gatacgaccc ctatcaggaa
     2581 atattcgagt ggcaactgca atgccacctg ggacagcaag accaggttct cgtggttgtc
     2641 ccatagggac tggtggagtt ctgtcttctc aaatcaaagt tgcccatcgc cctgtaacac
     2701 aacaaggttt gactggaatg aaaactggga cgaaaggtcc ccagaggcaa attttagaca
     2761 aatcttacta tcttgggctt cttagaagta aaataagtga acttacaact gaagttaata
     2821 aacttcagaa gggaatagaa atgtacaatc aagagaattc agtatatttg tcatatgaaa
     2881 agagggctga gactttagct gttgagataa aagagcttca aggacaacta gcagactaca
     2941 acatgttggt agataaactt aataccaaca ctgaaatgga agaagtaatg aatgattaca
     3001 atatgcttaa agctcaaaat gatcgagaaa cacaaagttt ggatgtcata tttactgaaa
     3061 gacaagcgaa agaaaaacaa atcagaagtg tcgaagaaga aattgaacag gaaaaacaag
     3121 caacagatga cattatcaaa aatatgtctt ttgaaaacca agtcaagtac ctagagatga
     3181 aaaccacaaa tgagaaactg ttacaggaat tagatacact tcaacaacaa ttggattcac
     3241 agaacatgaa aaaagagagc ctggaagcag aaatagctca ctcccaggtg aaacaggagg
     3301 cggtattgct gcatgaaaaa ctttatgagt tggagtccca tcgagatcaa atgattgcag
     3361 aagacaaaag cataggatct ccaatggaag agagagagaa attacttaag cagattaaag
     3421 atgataatca ggaaatagcc agcatggaaa gacagttaac agatacaaaa gaaaagataa
     3481 atcagtttat tgaagaaatt agacaacttg acatggattt agaggaacac caaggtgaaa
     3541 tgaaccagaa atacaaggag ctaaagaaaa gggaggaaca tatggacact tttattgaga
     3601 cttttgagga aacaaagaat caggaactga aacgaaaggc acagatagaa gccaacattg
     3661 ttgcactctt ggagcactgc agtcgaaata taaatcgtat agaacagata tcctctatca
     3721 ccaatcaaga gctaaagatg atgcaggatg acctcaattt taaatctact gaagtgcaga
     3781 aatcacaaag tacagctcag aatttgactt cagacattca acgtctgcag ttggatctgc
     3841 agaaaatgga gcttctagaa agtaagatga ctgaagaaca gcattctcta aaaagcaaaa
     3901 ttaagcaaat gacaactgat ctggagatat ataatgattt gccagcttta aaatcatcag
     3961 gtgaagaaaa gataaagaaa ttacatcagg agagaatgat attatcaacc cacagaaatg
     4021 cctttaagaa aataatggag aagcaaaaca tagagtatga ggcactaaaa acacaattgc
     4081 aagaaaatga gacacattct cagcttacaa atttggagag aaagtggcaa caccttgagc
     4141 aaaataattt tgcgatgaaa gaattcatag caaccaagag tcaagagagt gattaccagc
     4201 caattaagaa aaatgtgacc aagcagattg cagagtacaa taaaaccatc gtggatgctt
     4261 tacatagcac cagcggaaac tgactcgagg gtaccgcggg cccgggatcc accggatcta
     4321 gataactgat cacaattcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     4381 gtggccaatg ccctggctca caaataccac tgagatcgat ctttttccct ctgccaaaaa
     4441 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     4501 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     4561 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     4621 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     4681 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     4741 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     4801 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     4861 gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4921 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4981 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5041 tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt
     5101 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     5161 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     5221 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg
     5281 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     5341 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     5401 gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5461 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5521 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5581 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5641 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5701 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5761 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5821 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5881 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5941 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6001 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6061 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6121 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6181 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6241 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6301 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6361 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     6421 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     6481 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     6541 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     6601 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     6661 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     6721 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     6781 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6841 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6901 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6961 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7021 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7081 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7141 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7201 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7261 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7321 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7381 cccgaaaagt gccacctggg tcgagcagtg tggttttcaa gaggaagcaa aaagcctctc
     7441 cacccaggcc tggaatgttt ccacccaatg tcgagcagtg tggttttgca agaggaagca
     7501 aaaagcctct ccacccaggc ctggaatgtt tccacccaat gtcgagcaaa ccccgcccag
     7561 cgtcttgtca ttggcgaatt aattcgaaca cgcagatgca gtcggggcgg cgcggtccca
     7621 ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga ccctgcagcc
     7681 aatatgggat cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7741 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7801 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7861 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     7921 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     7981 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8041 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8101 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8161 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8221 cgcatgcccg acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8281 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8341 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8401 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8461 tatcgccttc ttgacgagtt cttctgaggg gatcggcaat aaaaagacag aataaaacgc
     8521 acgggtgttg ggtcgtttgt tcggatcc
//
