LOCUS       sequence-434356-        8777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally EGFP-tagged IFT81 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Ishida Y, Tasaki K, Katoh Y, Nakayama K
  TITLE     Molecular basis underlying the ciliary defects caused by IFT52 
            variations found in skeletal ciliopathies.
  JOURNAL   Mol Biol Cell. 2022 Aug 1;33(9):ar83. doi: 10.1091/mbc.E22-05-0188. 
            Epub 2022 Jun 15.
  PUBMED    35704471
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2022-08-1"; volume: "33(9):ar83. doi"; pages: " 
            10.1091/mbc.E22-05-0188. Epub 2022 Jun 15"
COMMENT     Sequence Label: pCAG-EGFP-C-IFT81
FEATURES             Location/Qualifiers
     source          1..8777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1744..1753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1750..1752,1753..1755,1756..2466)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1750..1752,1753..1755,1756..2466)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1795..1816)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2056..2075)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2403..2424
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4649..4668)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4714..4769
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4768..4787)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5130..5146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5130..5146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5143..5165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5154..5170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5178..5184,5185..5202,5203..5208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5223..5244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5303..5498
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5349..5484
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5504..5638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5541..5560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5595..5614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5706..5723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5877..6465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5957..5976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6636..7427,7428..7496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7259..7278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7497..7601)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7913..8716
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7976..7995)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8586..8605
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8723..8771
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa tttgctagtt
     1741 ttcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1801 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1861 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1921 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1981 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2041 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2101 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2161 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2221 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2281 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2341 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2401 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2461 tacaagtccg gactcagatc tatgagtgat caaattaaat tcattatgga cagtctcaat
     2521 aaggagccct ttaggaagaa ctataattta atcacgtttg attccttgga gccaatgcaa
     2581 ctattacaag ttctcagtga tgttctggct gagattgacc caaagcaact tgtggatatc
     2641 agagaggaga tgccagagca gacagccaaa cgaatgttga gccttcttgg tattcttaag
     2701 tacaaacctt caggaaatgc cacagatatg agtacttttc gtcagggttt ggtgattgga
     2761 agtaaacctg taatttaccc agtgctccac tggcttcttc agaggactaa tgaactgaag
     2821 aaaagagcat atttagctcg ttttttaata aaacttgagg taccaagtga gtttcttcag
     2881 gatgaaactg tggctgacac caataaacag tatgaagagt taatggaagc ctttaaaact
     2941 ttgcataaag aatatgagca gctcaagata tctggatttt ctacagcaga aataagaaag
     3001 gatatcagtg caatggaaga agaaaaggat cagctcatta agagagttga acatttgaag
     3061 aaaagggttg agacagctca gaatcatcaa tggatgctta aaatagcaag gcaacttcga
     3121 gttgaaaaag agagagaaga atatcttgca caacagaaac aggaacaaaa gaatcagcta
     3181 tttcatgcag tgcaaagatt gcaaagagta caaaaccagc tgaaaagcat gcgccaagct
     3241 gcagcagatg caaagcctga aagtttaatg aagaggctag aggaggagat aaaatttaat
     3301 ttatatatgg taactgaaaa atttcctaaa gaattagaaa ataagaaaaa ggaattacat
     3361 tttttacaaa aagtagtttc agagccagct atgggccatt ctgatcttct tgaacttgaa
     3421 tctaaaataa atgaaataaa cacagaaatt aaccagttga ttgaaaagaa aatgatgaga
     3481 aatgagccca ttgaaggcaa actctcactg tataggcaac aggcatctat catttcccgt
     3541 aaaaaagaag ccaaagctga ggaacttcag gaggccaagg agaagttagc cagcctagag
     3601 agagaagcat cagtaaagag aaatcagacc cgtgaatttg atggtactga agttttaaag
     3661 ggagatgagt tcaaacgata tgtcaataaa cttcgaagca agagtacagt tttcaaaaag
     3721 aagcatcaga taatagctga acttaaagct gaattcggtc ttttgcagag gactgaagaa
     3781 cttcttaagc aacgtcatga aaatattcaa caacaactgc aaactatgga ggagaaaaag
     3841 ggtatatctg gatatagtta cacccaagaa gagctagaaa gagtatctgc actgaagagt
     3901 gaagttgatg aaatgaaagg acgaacattg gatgatatgt ctgaaatggt gaaaaaactg
     3961 tattcattgg tatctgaaaa gaagtcagct cttgcctcag ttataaaaga gctacgacag
     4021 ttgcgtcaaa aatatcaaga actgacccag gagtgtgatg aaaagaaatc ccagtatgat
     4081 agctgtgcag caggcctcga aagcaatcgg tccaaattag aacaggaagt tagaagactc
     4141 cgtgaagaat gtcttcaaga agaaagtaga taccattata caaattgtat gattaagaac
     4201 ctagaagttc aacttcgtcg tgctactgat gagatgaagg catatatctc ttctgatcaa
     4261 caagaaaaaa gaaaggcaat tagggaacag tataccaaaa atactgctga acaagaaaac
     4321 cttggaaaga aacttcggga aaaacaaaaa gttatacgag aaagtcatgg tccaaatatg
     4381 aaacaagcaa aaatgtggcg tgatttggaa caattaatgg aatgtaagaa acagtgcttt
     4441 ctgaaacaac aaagccaaac ttccattggt caggtaattc aggagggtgg ggaggaccgg
     4501 ctaatactgt gactcgaggg taccgcgggc ccgggatcca ccggatctag ataactgatc
     4561 acaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     4621 cctggctcac aaataccact gagatcgatc tttttccctc tgccaaaaat tatggggaca
     4681 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     4741 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     4801 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     4861 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     4921 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     4981 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     5041 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     5101 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5161 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5221 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5281 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     5341 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     5401 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     5461 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     5521 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     5581 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     5641 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5701 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5761 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5821 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5881 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5941 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6001 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6061 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6181 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6241 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6301 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6361 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6421 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6481 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6541 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6601 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6661 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6721 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6781 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6841 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6901 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6961 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7021 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7081 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7141 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7201 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7261 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7321 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7381 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7441 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7501 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7561 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7621 ccacctgggt cgagcagtgt ggttttcaag aggaagcaaa aagcctctcc acccaggcct
     7681 ggaatgtttc cacccaatgt cgagcagtgt ggttttgcaa gaggaagcaa aaagcctctc
     7741 cacccaggcc tggaatgttt ccacccaatg tcgagcaaac cccgcccagc gtcttgtcat
     7801 tggcgaatta attcgaacac gcagatgcag tcggggcggc gcggtcccag gtccacttcg
     7861 catattaagg tgacgcgtgt ggcctcgaac accgagcgac cctgcagcca atatgggatc
     7921 ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     7981 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     8041 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     8101 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     8161 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     8221 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     8281 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     8341 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     8401 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     8461 cggcgatgat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     8521 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     8581 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     8641 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     8701 tgacgagttc ttctgagggg atcggcaata aaaagacaga ataaaacgca cgggtgttgg
     8761 gtcgtttgtt cggatcc
//
