LOCUS       sequence-434357-        9249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses N-terminally EGFP-tagged IFT88 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Ishida Y, Tasaki K, Katoh Y, Nakayama K
  TITLE     Molecular basis underlying the ciliary defects caused by IFT52 
            variations found in skeletal ciliopathies.
  JOURNAL   Mol Biol Cell. 2022 Aug 1;33(9):ar83. doi: 10.1091/mbc.E22-05-0188. 
            Epub 2022 Jun 15.
  PUBMED    35704471
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2022-08-1"; volume: "33(9):ar83. doi"; pages: " 
            10.1091/mbc.E22-05-0188. Epub 2022 Jun 15"
COMMENT     Sequence Label: pCAG-EGFP-C-IFT88
FEATURES             Location/Qualifiers
     source          1..9249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..663
                     /label=chicken beta-actin promoter
     intron          664..1680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1604..1626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1688..1707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1745..1754
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1751..1753,1754..1756,1757..2467)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1751..1753,1754..1756,1757..2467)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1796..1817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2057..2076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2404..2425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5121..5140)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5186..5241
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5240..5259)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5602..5618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5602..5618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5615..5637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5626..5642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5650..5656,5657..5674,5675..5680))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5695..5716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5775..5970
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5821..5956
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5976..6110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6013..6032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6067..6086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6178..6195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6349..6937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6429..6448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7108..7899,7900..7968))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7731..7750
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7969..8073)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8385..9188
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8448..8467)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9058..9077
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9195..9243
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
      541 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
      841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga atttgctagt
     1741 tttcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1801 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1861 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1921 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1981 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2041 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2101 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2161 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     2221 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2281 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2341 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2401 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2461 gtacaagtcc ggactcagat ccatgaaatt cacaaacact aaggtacaaa tgatgcaaaa
     2521 tgtgcacctg gctccagaga cagatgaaga tgatctttat tccggctata atgactacaa
     2581 tccaatctat gatatcgagg aattggagaa tgatgcagct tttcagcaag ctgtgaggac
     2641 tagtcatggc agaagacctc caataactgc taaaatatca agcacggcag ttactagacc
     2701 tatagctact ggatatgggt ccaagacatc tctggcatca tcaataggaa gaccaatgac
     2761 aggggctatt caggatggag ttactagacc catgacagca gtgagagcag ctggttttac
     2821 caaagcagct ttgagaggct ctgcatttga cccccttagt cagtcaaggg gccctgcttc
     2881 ccctttggaa gccaagaaaa aagatagccc agaggaaaaa ataaagcaat tagagaagga
     2941 agtaaatgag ttggtagaag aaagctgtat tgccaatagt tgtggagact taaaattggc
     3001 cttagaaaag gcaaaagatg caggaagaaa agagagagtc ctggtgagac agcgagaaca
     3061 agttacaact ccagaaaata tcaatttgga tttaacttac tcagttcttt tcaatttggc
     3121 cagtcagtat tcagttaatg aaatgtatgc cgaagcactt aacacttatc aagttatagt
     3181 caaaaataag atgtttagca atgcaggaat attgaaaatg aatatgggaa atatctattt
     3241 aaagcaaaga aattattcca aagccattaa attctaccga atggcattag accaagttcc
     3301 aagtgtcaat aagcaaatga ggattaaaat aatgcagaat attggagtta catttattca
     3361 ggctggtcag tattcagatg ctattaattc atatgagcac ataatgagca tggcaccaaa
     3421 tctgaaggca ggctacaacc taactatctg ttattttgct attggagacc gagaaaaaat
     3481 gaagaaggca ttccaaaaat tgattactgt tccattagaa attgatgaag ataaatatat
     3541 ttcaccaagt gatgatcctc atactaactt agtaactgaa gctataaaaa atgatcacct
     3601 caggcaaatg gaacgtgaaa ggaaagccat ggcagaaaaa tatattatga catctgcaaa
     3661 actcattgct cctgtaattg aaacatcttt tgctgcaggt tatgattggt gcgtggaagt
     3721 ggtgaaagct tctcaatatg tagagctagc caatgatctg gaaataaaca aagcagttac
     3781 atacttgaga caaaaagact ataaccaagc tgtagagatc ttaaaagtgt tggaaaaaaa
     3841 ggacagtaga gtgaaaagtg cagctgcaac caatctctca gccctgtatt atatgggaaa
     3901 ggattttgca caagccagca gctatgcaga tatagctgtg aactctgata gatataatcc
     3961 agcagctctt actaataaag ggaatacagt ttttgcaaat ggtgattatg agaaggccgc
     4021 tgaattctat aaagaggctc taagaaatga ttcttcttgt actgaagcac tttataatat
     4081 tggccttacc tatgagaaac taaatcggct agatgaggct ttggactgtt tcctgaaact
     4141 tcacgcaatc ctacgaaaca gtgccgaagt tctttaccag atagcaaata tatatgaatt
     4201 aatggaaaat cccagtcaag ctattgaatg gctaatgcag gtggtcagtg ttattccaac
     4261 cgatcctcaa gttttatcta agctaggaga attatatgat cgtgaaggag ataaatctca
     4321 agcatttcaa tattactatg agtcatatag gtattttcct tgtaatattg aagtcattga
     4381 gtggcttgga gcctattaca ttgacaccca attttgggaa aaagctattc agtactttga
     4441 aagagcttct cttatacagc ctacacaagt gaaatggcag ctgatggtag ctagttgttt
     4501 cagaagaagt ggtaactacc aaaaagcatt agatacttac aaagatactc acagaaaatt
     4561 tccagaaaat gtcgaatgtc tgcgtttctt agttcgtctc tgcacagatc ttggattaaa
     4621 agatgctcaa gaatatgcca gaaaactgaa gaggttggaa aaaatgaaag aaataaggga
     4681 acagcgcata aagtcaggca gagatggcag tgggggctcc cgtggcaaaa gagaaggaag
     4741 tgctagcggt gatagtggcc agaactatag tgccagtagt aaaggtgaac gactaagtgc
     4801 cagactcaga gctttacctg ggacaaatga accttatgaa agtagcagta acaaagaaat
     4861 agatgcctcc tatgtggacc cacttggccc tcaaatagaa cgaccaaaaa ctgcagccaa
     4921 gaaaaggatc gatgaggatg attttgctga tgaagaatta ggagatgatt tgcttccaga
     4981 ataactcgag ggtaccgcgg gcccgggatc caccggatct agataactga tcacaattca
     5041 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     5101 acaaatacca ctgagatcga tctttttccc tctgccaaaa attatgggga catcatgaag
     5161 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     5221 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     5281 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     5341 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     5401 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     5461 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     5521 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag
     5581 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5641 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5701 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5761 gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5821 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5881 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     5941 ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg
     6001 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6061 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta
     6121 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6181 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6241 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6301 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6361 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6421 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6481 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6541 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6601 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6661 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6721 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6781 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6841 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6901 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6961 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7021 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7081 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7141 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7201 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7261 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7321 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7381 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7441 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7501 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7561 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7621 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7681 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7741 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7801 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7861 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7921 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7981 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8041 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
     8101 gtcgagcagt gtggttttca agaggaagca aaaagcctct ccacccaggc ctggaatgtt
     8161 tccacccaat gtcgagcagt gtggttttgc aagaggaagc aaaaagcctc tccacccagg
     8221 cctggaatgt ttccacccaa tgtcgagcaa accccgccca gcgtcttgtc attggcgaat
     8281 taattcgaac acgcagatgc agtcggggcg gcgcggtccc aggtccactt cgcatattaa
     8341 ggtgacgcgt gtggcctcga acaccgagcg accctgcagc caatatggga tcggccattg
     8401 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     8461 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     8521 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
     8581 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     8641 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     8701 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     8761 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     8821 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     8881 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgatg
     8941 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     9001 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     9061 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     9121 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     9181 tcttctgagg ggatcggcaa taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg
     9241 ttcggatcc
//
