LOCUS       sequence-434358-        9662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector with lacIq-Plac expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Intorcia V, Sava RL, Schroeder GP, Gebhardt MJ
  TITLE     A series of vectors for inducible gene expression in 
            multidrug-resistant Acinetobacter baumannii.
  JOURNAL   Appl Environ Microbiol. 2024 Sep 18;90(9):e0047424. doi: 
            10.1128/aem.00474-24. Epub 2024 Aug 20.
  PUBMED    39162403
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2024-09-18"; volume: "90(9):e0047424. 
            doi"; pages: " 10.1128/aem.00474-24. Epub 2024 Aug 20"
COMMENT     Sequence Label: pMApra-Plac-SO
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..840
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             827..1678
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(1955..3037)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2999..3018
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(3038..3115)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    3353..3369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      3516..3602
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3694..3721
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3740..3831
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4274..4376
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4443..4462)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4789..5589
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV     
                               "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies, 
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     rep_origin      6119..6513
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            6855..6942
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             8181..9152
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1981 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2041 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     2101 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     2161 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     2221 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     2281 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     2341 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     2401 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     2461 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     2521 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     2581 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     2641 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2701 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2761 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2821 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     2881 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     2941 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     3001 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     3061 cttccgggcg ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt
     3121 aaatgccgct tcgccttcgc gcgcgaattg caagctgatc cgggcttatc gactgcacgg
     3181 tgcaccaatg cttctggcgt caggcagcca tcggaagctg tggtatggct gtgcaggtcg
     3241 taaatcactg cataattcgt gtcgctcaag gcgcactccc gttctggata atgtttttct
     3301 cgttccctgc tgcctgacgt gagctcattt attctgaaat gagctcttga aattgtgagc
     3361 ggataacaat tataattcta gagacgtcga attcctgcag aagcttacta gtggctgaat
     3421 cccgaaacag ttcgcaggta atagttagag cctgcataac ggtttcggga ttttttatag
     3481 gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga
     3541 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     3601 atccgccggg agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac
     3661 gcccgccata aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt
     3721 ttgcgtttct acaaactctt ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3781 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3841 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3901 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3961 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4021 gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac
     4081 agctgataga aacagaagcc actggcctca aaaacaccat catacactaa atcagtaagt
     4141 tggcagcatc acccgacgca ctttgcgccg aataaatacc tgtgacggaa gatcacttcg
     4201 cagaataaat aaatcctggt gtccctgttg ataccgggaa gccctgggcc aacttttggc
     4261 gaaaatgaga cgttgatcgg cacgtaagag gttccaactt tcaccataat gaaataagat
     4321 cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg
     4381 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     4441 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     4501 cggccttttt atcaaggccc gatccttgga gcccttgccc tcccgcacga tgatcgtgcc
     4561 gtgatcgaaa tccagatcct tgacccgcag ttgcaaaccc tcactgatcc ggctcacggt
     4621 aactgatgcc gtatttgcag taccagcgta cggcccacag aatgatgtca cgctgaaaat
     4681 gccggccttt gaatgggttc atgtgcagct ccatcagcaa aaggggatga taagtttatc
     4741 accacctact atttgcaaca gtgccgttga tcgtgctatg atcgactgat gtcatcagcg
     4801 gtggagtgca atgtcgtgca atacgaatgg cgaaaagccg agctcatcgg tcagcttctc
     4861 aaccttgggg ttacccccgg cggtgtgctg ctggtccaca gctccttccg tagcgtccgg
     4921 cccctcgaag atgggccact tggactgatc gaggccctgc gtgctgcgct gggtccggga
     4981 gggacgctcg tcatgccctc gtggtcaggt ctggacgacg agccgttcga tcctgccacg
     5041 tcgcccgtta caccggacct tggagttgtc tctgacacat tctggcgcct gccaaatgta
     5101 aagcgcagcg cccatccatt tgcctttgcg gcagcggggc cacaggcaga gcagatcatc
     5161 tctgatccat tgcccctgcc acctcactcg cctgcaagcc cggtcgcccg tgtccatgaa
     5221 ctcgatgggc aggtacttct cctcggcgtg ggacacgatg ccaacacgac gctgcatctt
     5281 gccgagttga tggcaaaggt tccctatggg gtgccgagac actgcaccat tcttcaggat
     5341 ggcaagttgg tacgcgtcga ttatctcgag aatgaccact gctgtgagcg ctttgccttg
     5401 gcggacaggt ggctcaagga gaagagcctt cagaaggaag gtccagtcgg tcatgccttt
     5461 gctcggttga tccgctcccg cgacattgtg gcgacagccc tgggtcaact gggccgagat
     5521 ccgttgatct tcctgcatcc gccagaggcg ggatgcgaag aatgcgatgc cgctcgccag
     5581 tcgattggct gagctcatga aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     5641 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     5701 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     5761 tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattgtct tcaaattccc
     5821 gttgcacata gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt
     5881 aatactcgtc aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac
     5941 cagtatcccg atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg
     6001 caatactgtg tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa
     6061 atgcctgccg ttgctagaca ttgccagcca gtgcccgtca ctcccgtact aactgtcacg
     6121 aacccctgca ataactgtca cgcccccctg caataactgt cacgaacccc tgcaataact
     6181 gtcacgcccc caaacctgca aacccagcag gggcgggggc tggcggggtg ttggaaaaat
     6241 ccatccatga ttatctaaga ataatccact aggcgcggtt atcagcgccc ttgtggggcg
     6301 ctgctgccct tgcccaatat gcccggccag aggccggata gctggtctat tcgctgcgct
     6361 aggctacaca ccgccccacc gctgcgcggc agggggaaag gcgggcaaag cccgctaaac
     6421 cccacaccaa accccgcaga aatacgctgg agcgctttta gccgctttag cggcctttcc
     6481 ccctacccga agggtggggc cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt
     6541 cagcccggct catccttctg gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt
     6601 tcaaggtacg catccattgc cgccatgagc cgatcctccg gccactcgct gctgttcacc
     6661 ttggccaaaa tcatggcccc caccagcacc ttgcgccttg tttcgttctt gcgctcttgc
     6721 tgctgttccc ttgcccgcac ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg
     6781 agcttggcca gccgatccgc cgccttgttg ctccccttaa ccatcttgac accccattgt
     6841 taatgtgctg tctcgtaggc tatcatggag gcacagcggc ggcaatcccg accctacttt
     6901 gtaggggagg gcgcacttac cggtttctct tcgagaaact ggcctaacgg ccacccttcg
     6961 ggcggtgcgc tctccgaggg ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca
     7021 gcatggcgat ttatcacctt acggcgaaaa ccggcagcag gtcgggcggc caatcggcca
     7081 gggccaaggc cgactacatc cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct
     7141 tgcacgccga atccgggcac atgccggagt tcgtcgagcg gcccgccgac tactgggatg
     7201 ctgccgacct gtatgaacgc gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc
     7261 cggtcgagct gaccctcgac cagcagaagg cgctggcgtc cgagttcgcc cagcacctga
     7321 ccggtgccga gcgcctgccg tatacgctgg ccatccatgc cggtggcggc gagaacccgc
     7381 actgccacct gatgatctcc gagcggatca atgacggcat cgagcggccc gccgctcagt
     7441 ggttcaagcg gtacaacggc aagaccccgg agaagggcgg ggcacagaag accgaagcgc
     7501 tcaagcccaa ggcatggctt gagcagaccc gcgaggcatg ggccgaccat gccaaccggg
     7561 cattagagcg ggctggccac gacgcccgca ttgaccacag aacacttgag gcgcagggca
     7621 tcgagcgcct gcccggtgtt cacctggggc cgaacgtggt ggagatggaa ggccggggca
     7681 tccgcaccga ccgggcagac gtggccctga acatcgacac cgccaacgcc cagatcatcg
     7741 acttacagga ataccgggag gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc
     7801 agaggcatca acgagttagc ggagcagatc gaaccgctgg cccagagcat ggcgacactg
     7861 gccgacgaag cccggcaggt catgagccag acccagcagg ccagcgaggc gcaggcggcg
     7921 gagtggctga aagcccagcg ccagacaggg gcggcatggg tggagctggc caaagagttg
     7981 cgggaggtag ccgccgaggt gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg
     8041 tggcactgga agctatggct aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg
     8101 ctgatcgcat cgttgctctt gctcgacctg acgccactga caaccgagga cggctcgatc
     8161 tggctgcgct tggtggcccg atgaagaacg acaggacttt gcaggccata ggccgacagc
     8221 tcaaggccat gggctgtgag cgcttcgata tcggcgtcag ggacgccacc accggccaga
     8281 tgatgaaccg ggaatggtca gccgccgaag tgctccagaa cacgccatgg ctcaagcgga
     8341 tgaatgccca gggcaatgac gtgtatatca ggcccgccga gcaggagcgg catggtctgg
     8401 tgctggtgga cgacctcagc gagtttgacc tggatgacat gaaagccgag ggccgggagc
     8461 ctgccctggt agtggaaacc agcccgaaga actatcaggc atgggtcaag gtggccgacg
     8521 ccgcaggcgg tgaacttcgg gggcagattg cccggacgct ggccagcgag tacgacgccg
     8581 acccggccag cgccgacagc cgccactatg gccgcttggc gggcttcacc aaccgcaagg
     8641 acaagcacac cacccgcgcc ggttatcagc cgtgggtgct gctgcgtgaa tccaagggca
     8701 agaccgccac cgctggcccg gcgctggtgc agcaggctgg ccagcagatc gagcaggccc
     8761 agcggcagca ggagaaggcc cgcaggctgg ccagcctcga actgcccgag cggcagctta
     8821 gccgccaccg gcgcacggcg ctggacgagt accgcagcga gatggccggg ctggtcaagc
     8881 gcttcggtga tgacctcagc aagtgcgact ttatcgccgc gcagaagctg gccagccggg
     8941 gccgcagtgc cgaggaaatc ggcaaggcca tggccgaggc cagcccagcg ctggcagagc
     9001 gcaagcccgg ccacgaagcg gattacatcg agcgcaccgt cagcaaggtc atgggtctgc
     9061 ccagcgtcca gcttgcgcgg gccgagctgg cacgggcacc ggcaccccgc cagcgaggca
     9121 tggacagggg cgggccagat ttcagcatgt agtgcttgcg ttggtactca cgcctgttat
     9181 actatgagta ctcacgcaca gaagggggtt ttatggaata cgaaaaaagc gcttcagggt
     9241 cggtctacct gatcaaaagt gacaagggct attggttgcc cggtggcttt ggttatacgt
     9301 caaacaaggc cgaggctggc cgcttttcag tcgctgatat ggccagcctt aaccttgacg
     9361 gctgcacctt gtccttgttc cgcgaagaca agcctttcgg ccccggcaag tttctcggtg
     9421 actgatatga aagaccaaaa ggacaagcag accggcgacc tgctggccag ccctgacgct
     9481 gtacgccaag cgcgatatgc cgagcgcatg aaggccaaag ggatgcgtca gcgcaagttc
     9541 tggctgaccg acgacgaata cgaggcgctg cgcgagtgcc tggaagaact cagagcggcg
     9601 cagggcgggg gtagtgaccc cgccagcgcc taaccaccaa ctgcctgcaa aggaggcaat
     9661 ca
//
