LOCUS       sequence-434370-        6427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli-Bacteroides shuttle vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Yeh YH, Kelly VW, Rahman Pour R, Sirk SJ
  TITLE     A molecular toolkit for heterologous protein secretion across 
            Bacteroides species.
  JOURNAL   Nat Commun. 2024 Nov 11;15(1):9741. doi: 10.1038/s41467-024-53845-7.
  PUBMED    39528443
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-11-11"; volume: "15(1):9741. doi"; pages: " 
            10.1038/s41467-024-53845-7"
COMMENT     Sequence Label: pYHY1
FEATURES             Location/Qualifiers
     source          1..6427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(2096..2887,2888..2956))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2719..2738
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2957..3061)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3164..3752
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3653..3672
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            3912..4021
                     /note="incP origin of transfer"
     CDS             4054..4425
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ttggacgcag ccgaatggga aaagtgtgcg tttatcgcgt gatgacatgg cagaactcca
       61 aaccatagca tctgaaacac tgggcatgga acgtggcgtt tcttctgacc gcaaacactt
      121 atcggctatg cagtacaaga ccgaatgtgc gaaagaacag cttcaggaac tatccaacga
      181 tatatccagt gcattagaca aatacaagga cgcacaaaac cagcttcttc agcttcagaa
      241 ggaactacgt tccattgaaa caaagaagaa cgtccaaaaa ctcatttcta aggcttcaga
      301 gaagttttac ggcttgattg gaaaaacagt caacgatagg gaaaaggatg ccttaaaagc
      361 caaagtaaag actttagagg gggaaaatga acaactatcc gatagactgg gaaaggctat
      421 acttgaaaaa gagcgaaaca gcacaaaggc attcaaagcc gagaatgaca aggagtatta
      481 tagacaacaa atggacaacg caaggacaac cagtaaccta ttaaggacgg agaaccaaaa
      541 actaaaagcc gaaaccaagg agttgaaaaa agaacttggt aagatgaaag acttgttcaa
      601 ttccgaacaa ctggaggctt taaggcatca tttcccaaac atatcaaaag ctatggaaga
      661 ggggaaagac ctattcaagc aaatcaccag aagcagaggt ttcggcatgg gtatgtaacc
      721 cccccccgcc cccaaagggg gagcgaccaa acggcagcct ctctcaatgg agtgttacat
      781 cccgtaaaga ggggctttgc agggggtgga ctcagaaaga tgagaataga tgactattgt
      841 agttgaaaca catagaaagt tgctgatata cagaccgata cgcatatcgg gatgaaccat
      901 gagtacgttc ttttctcaaa aaacataaat attcgaaaag agatgcaata aattaaggag
      961 aggttataat gaacaaagta aatataaaag atagtcaaaa ttttattact tcaaaatatc
     1021 acatagaaaa aataatgaat tgcataagtt tagatgaaaa agataacatc tttgaaatag
     1081 gtgcagggaa aggtcatttt actgctggat tggtaaagag atgtaatttt gtaacggcga
     1141 tagaaattga ttctaaatta tgtgaggtaa ctcgtaataa gctcttaaat tatcctaact
     1201 atcaaatagt aaatgatgat atactgaaat ttacatttcc tagccacaat ccatataaaa
     1261 tatttggcag cataccttac aacataagca caaatataat tcgaaaaatt gtttttgaaa
     1321 gttcagccac aataagttat ttaatagtgg aatatggttt tgctaaaatg ttattagata
     1381 caaacagatc actagcattg ctgttaatgg cagaggtaga tatttctata ttagcaaaaa
     1441 ttcctaggta ttatttccat ccaaaaccta aagtggatag cacattaatt gtattaaaaa
     1501 gaaagccagc aaaaatggca tttaaagaga gaaaaaaata tgaaactttt gtaatgaaat
     1561 gggttaacaa agagtacgaa aaactgttta caaaaaatca atttaataaa gctttaaaac
     1621 atgcgagaat atatgatata aacaatatta gtttcgaaca atttgtatcg ctatttaata
     1681 gttataaaat atttaacggc taaaaacaat aggccacatg caactgtaaa tgtttacgcg
     1741 tcctcggtac caacaaatac tttcaggacg atgtaaagtc ctgaaaaatc aaagctggcc
     1801 aatgagacga cggggtcatc acggctcatc atgcgccaaa caaatgtgtg caatacacgc
     1861 tcggatgact gcatgatgac cgcactgact ggggacagca gatccaccta agcctgtgag
     1921 agaagcagac acccgacaga tcaaggcagt taactagtgc actgcagtgg cgtcgacgga
     1981 tccgaattct acagcggccg cgattatcaa aaaggatctt cacctagatc cttttaaatt
     2041 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2101 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2161 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2221 ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2281 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2341 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2401 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2461 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2521 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2581 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2641 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2701 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2761 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2821 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2881 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2941 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3001 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3061 gcacatttcc ccgaaaagtg ccacctgtca tgaccaaaat cccttaacgt gagttttcgt
     3121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     3181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     3241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     3301 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     3361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     3421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3781 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3841 tggataaccg tgcggccgcc cctccaaagg gttcgtgtag actttccttg gtgtatccaa
     3901 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     3961 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     4021 gctaccgccg gcgtaacaga tgagggcaag cggatggctg atgaaaccaa gccaaccagg
     4081 aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc gattgaggaa
     4141 aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg ccagggctac
     4201 aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat caatggcgac
     4261 ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg cacggcgcgg
     4321 ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca ggacgagctt
     4381 ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt ttagccgcta
     4441 aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc ataacgcata gcgctccccg
     4501 accgatgatt tttaagtgac tgattttgtg ctgttttggg ggtatattaa gaataaaaga
     4561 aatagaataa gttaagtact tgatacacaa tatagggcat tttccatatt ggaaattctc
     4621 attttccaat ctagaaaata ccgatttcct aatatgatac taaataggaa aataatattt
     4681 cccttaatat tgtttttatg gaaaataata gtttactttg tggagaataa tatttcccaa
     4741 aaacatatca aaatggaaaa taaaaaagca gttaagttaa ccgattttca aaagaacgaa
     4801 gaaaatcctt ttatgaaaca agctatagaa ggtattgaaa atcatgttgt taaaaagtat
     4861 aagagtaata gtggtggcga taagagagct gtagtagctt tagccgacac tgaaactgga
     4921 gaagtgttta agacttcgtt tatccgtcaa atagaagtag atgaagaaca attcactaaa
     4981 ttgtatcttt ctaactttgc tgcattcttt gacctatcac aagcagctat tcgggttttt
     5041 ggttacttta tgacctgcat gaaacccaaa aatgatttaa tcattttcaa tagaaaaaaa
     5101 tgcctagaat ataccaaata caaaacagac aaagccgttt ataaaggact tgcagaactt
     5161 gtaaaagctg aaatcatagc ccgaggacca gccgataatc tttggtttat taatcctctg
     5221 atagtattca atggtgaccg agtgacattt gctaaaacat acgttcggaa aaagacttta
     5281 gctgcccaaa agaaagaaga agcagagaaa cgacaattat cacttggctt tgatgaacag
     5341 taacactcca ttgagtgaag ctgccgtttg gtcgctcccc ctttgggggc ggggggggat
     5401 agataaagtt cctctatgta aagttataat gggggatgaa aggcaaggtt cgctaacctt
     5461 accccgattg tttatgcttc ccagcattca atctatcccc gtttatgggt ttctctttca
     5521 ccctacaaag ataacctcat gggggaaaaa tgtccaagaa tatggggtaa aaactatcaa
     5581 gtcggtagaa aataagtatc tttgaagtac atttttagta gaggtactgg tgtgcctagg
     5641 cgaagcagat aggcaaaaat aaagccgctg gagatgtcgg atgtccgtca attaactcca
     5701 gcggcttgta ttttggtaac tctcccgtat cggtcagcta ccgtttggtc agcaacaaag
     5761 gtagtacttt attatcgcaa atccaaatgt ttttcccttt atttgtgcat ttacaaaaca
     5821 ttcactaact tcgcacctat gcagtatggt gatagcacac tagcacccta aagagtgcgt
     5881 gtgtaagggg acaagccccc ttaaccccct gtcagccggc tgtcaccggc tgttatcaat
     5941 cttcaaagtc gggtggttgt caagatgatt tttttggttt ggtgtcgtct ttttttaagc
     6001 tgccgcataa cggtttgcga ttgtcacaaa aatgttaact tatgggagca acaagtattc
     6061 atgtacaagc agtgaagccg gggagcgaga ttcacaactt tagggaaaaa gagttggact
     6121 atgttcgtcc cgaacttagt catttgaatg aaagctgggt tggagatagc atttcccatc
     6181 ggctggagag tgcaaaacaa agatatctcg atacggttgg gcagaagatg caggctaaag
     6241 ccgcacccat acgagaggga gtaatagtaa tcaaacaaga aaccactatg caggaactcc
     6301 agcagtttgc cacggtctgc aaagaacgtt tcggtatcga agcgtttcaa atccatatac
     6361 acaaggacga aggatacatg aacgcaaagc agtggacacc taaccttcat gcccatgtag
     6421 ttttcga
//
