LOCUS       sequence-434373-        5591 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human sdi1 (p21 CDK inhibitor).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Noda A, Ning Y, Venable SF, Pereira-Smith OM, Smith JR
  TITLE     Cloning of senescent cell-derived inhibitors of DNA synthesis using 
            an expression screen.
  JOURNAL   Exp Cell Res. 1994 Mar;211(1):90-8. doi: 10.1006/excr.1994.1063.
  PUBMED    8125163
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1006/excr.1994";
            journalName: "Exp Cell Res"; date: "1994-03"; volume: "211"; issue: 
            "1"; pages: "90-8"
COMMENT     Sequence Label: pCDSRaD-sdi1
FEATURES             Location/Qualifiers
     source          1..5591
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          112..208
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     CDS             348..842
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     polyA_signal    2420..2554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2444..2463)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2498..2517
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2697..2719)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2860..2879
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2994..3011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3165..3753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3245..3264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3924..4715,4716..4784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4547..4566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4785..4889)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4957..4975
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5045..5065)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(5050..5379,5380..5394,5395..5591,1..72)
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early 
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     rep_origin      5230..5365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             join(5395..5591,1..72)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
ORIGIN
        1 cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg
       61 ccgttacaga tccaagctcc tcgaggaact gaaaaaccag aaagttaact ggtaagttta
      121 gtctttttgt cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct
      181 cctcagtgga tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa
      241 gctgctgcag gtcgactcta gaggatcccc ctgccgaagt cagttccttg tggagccgga
      301 gctgggcgcg gattcgccga ggcaccgagg cactcagagg aggcgccatg tcagaaccgg
      361 ctggggatgt ccgtcagaac ccatgcggca gcaaggcctg ccgccgcctc ttcggcccag
      421 tggacagcga gcagctgagc cgcgactgtg atgcgctaat ggcgggctgc atccaggagg
      481 cccgtgagcg atggaacttc gactttgtca ccgagacacc actggagggt gacttcgcct
      541 gggagcgtgt gcggggcctt ggcctgccca agctctacct tcccacgggg ccccggcgag
      601 gccgggatga gttgggagga ggcaggcggc ctggcacctc acctgctctg ctgcagggga
      661 cagcagagga agaccatgtg gacctgtcac tgtcttgtac ccttgtgcct cgctcagggg
      721 agcaggctga agggtcccca ggtggacctg gagactctca gggtcgaaaa cggcggcaga
      781 ccagcatgac agatttctac cactccaaac gccggctgat cttctccaag aggaagccct
      841 aatccgccca caggaagcct gcagtcctgg aagcgcgagg gcctcaaagg cccgctctac
      901 atcttctgcc ttagtctcag tttgtgtgtc ttaattatta tttgtgtttt aatttaaaca
      961 cctcctcatg tacataccct ggccgccccc tgccccccag cctctggcat tagaattatt
     1021 taaacaaaaa ctaggcggtt gaatgagagg ttcctaagag tgctgggcat ttttatttta
     1081 tgaaatacta tttaaagcct cctcatcccg tgttctcctt ttcctctctc ccggaggttg
     1141 ggtgggccgg cttcatgcca gctacttcct cctccccact tgtccgctgg gtggtaccct
     1201 ctggaggggt gtggctcctt cccatcgctg tcacaggcgg ttatgaaatt cacccccttt
     1261 cctggacact cagacctgaa ttctttttca tttgagaagt aaacagatgg cactttgaag
     1321 gggcctcacc gagtgggggc atcatcaaaa actttggagt cccctcacct cctctaaggt
     1381 tgggcagggt gaccctgaag tgagcacagc ctagggctga gctggggacc tggtaccctc
     1441 ctggctcttg atacccccct ctgtcttgtg aaggcagggg gaaggtgggg tcctggagca
     1501 gaccaccccg cctgccctca tggcccctct gacctgcact ggggagcccg tctcagtgtt
     1561 gagccttttc cctctttggc tcccctgtac cttttgagga gccccagcta cccttcttct
     1621 ccagctgggc tctgcaattc ccctctgctg ctgtccctcc cccttgtcct ttcccttcag
     1681 taccctctca gctccaggtg gctctgaggt gcctgtccca cccccacccc cagctcaatg
     1741 gactggaagg ggaagggaca cacaagaaga agggcaccct agttctacct caggcagctc
     1801 aagcagcgac cgccccctcc tctagctgtg ggggtgaggg tcccatgtgg tggcacaggc
     1861 ccccttgagt ggggttatct ctgtgttagg ggtatatgat gggggagtag atctttctag
     1921 gagggagaca ctggcccctc aaatcgtcca gcgaccttcc tcatccaccc catccctccc
     1981 cagttcattg cactttgatt agcagcggaa caaggagtca gacattttaa gatggtggca
     2041 gtagaggcta tggacagggc atgccacgtg ggctcatatg gggctgggag tagttgtctt
     2101 tcctggcact aacgttgagc ccctggaggc actgaagtgc ttagtgtact tggagtattg
     2161 gggtctgacc ccaaacacct tccagctcct gtaacatact ggcctggact gttttctctc
     2221 ggctccccat gtgtcctggt tcccgtttct ccacctagac tgtaaacctc tcgagggcag
     2281 ggaccacacc ctgtactgtt ctgtgtcttt cacagctcct cccacaatgc tgaatataca
     2341 gcaggtgctc aataaatgat tcttagtgaa aaaaaagggt accttctgag gcggaaagaa
     2401 ccagccggat ccctcgaggg atccagacat gataagatac attgatgagt ttggacaaac
     2461 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     2521 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     2581 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     2641 tggtatggct gattatgatc cggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2701 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2761 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     2821 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     2881 tgagagtgca ccataggtcg accggtcgac cggtcgaccg gtcgacctat gcggtgtgaa
     2941 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     3001 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3061 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3121 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3181 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3241 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     3301 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     3361 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     3421 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3481 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3541 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3601 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3661 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3721 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3781 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3841 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3901 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3961 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4021 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4081 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4141 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4201 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4261 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     4321 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     4381 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     4441 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4501 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4561 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4621 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4681 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4741 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4801 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4861 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4921 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     4981 cgtcttcaag aattggtcga ccaattctca tgtttgacag cttatcatcg ataagcttgg
     5041 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5101 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5161 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5221 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5281 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5341 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga tcgaggggct
     5401 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     5461 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     5521 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     5581 agccggctct c
//
