LOCUS       sequence-434396-       11003 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of type III-A Csm complex from Streptococcus 
            thermophilus without NLS tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Nemudraia A, Nemudryi A, Wiedenheft B
  TITLE     Repair of CRISPR-guided RNA breaks enables site-specific RNA 
            excision in human cells.
  JOURNAL   Science. 2024 Apr 25:eadk5518. doi: 10.1126/science.adk5518.
  PUBMED    38662916
REFERENCE   2  (bases 1 to 11003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2024 Apr 25:eadk5518. doi: 10.1126/science.adk5518."
COMMENT     Sequence Label: pAN052
FEATURES             Location/Qualifiers
     source          1..11003
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(816..839)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      839..848
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(879..1082)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    1113..1160
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1836..1859)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1859..1868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1899..2102)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    2133..2180
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(2574..2597)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      2597..2606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2637..2840)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    2871..2918
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(5208..5231)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5238..5255)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(5295..5498)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     rep_origin      complement(5529..6117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5609..5628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6288..7079,7080..7148))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6911..6930
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7149..7253)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7321..7339
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     polyA_signal    7421..7468
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(7469..8068)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7552..7572)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     8049..8068
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(8105..8308)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    8339..8386
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(9131..9154)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      9154..9163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9194..9397)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    9428..9475
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(10562..10585)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      10585..10594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(10625..10828)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     polyA_signal    10859..10906
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ttgctgaagg tgctcccgga ggcgaacttg tacaggtcct gcttccgata gttttcgttg
       61 gtggcgtggg agaatgcgaa tccggacgac ttggtcaaca ggtaatggcc atcttccatc
      121 gcttcctcga gatcagcgtc caccgggagg gcggtggtaa gtgacatgac cttgtcgctg
      181 tggttcttcg tcagccggtc ggacagttcc aagggaatgt tctggatgtc cagctcaaat
      241 ctgccgaatc ctgaggacct tttgccgccc agcccggagt actgaaggct gctcatcagt
      301 tcgttcagca gatcggattc gttggcgatc acgtaaaggg aggtgtcgtt cgagaaccga
      361 gtggtggcca cttggtacag attatcatct ttatggggct ggttcttggt cactgtgtcg
      421 atcacggcgt gttcctcatt ctcaaacagc tctccgttca ggtagtcgtc gacgttttcc
      481 agagccagga actggagctt tttggacagc ttcgcttggc ggcggacttc cttcacgtcc
      541 acagactggt cgatctggtc gtgcttgggg taaccaatcg gtttcggcag gaaaggaccg
      601 aattggaatg ggaacgcgtc ggtcagagtg aacttatcct ggttggcctc ggccaagaag
      661 gcgtcgagct ttcccatctt cagagcctcg agcacgagtg ccgagaagat tctatccgcg
      721 gagaaggtca gcttcgatga gtccagagtg ccggaaccga agtgggcgtt ttgaaaggtc
      781 ataatgtaga gcttgtaagt catgccccgc gccatcttat cgtcatcgtc tttgtaatcc
      841 atggtggcgg ctctagagat ctgacggttc actaaacgag ctctgcttat atagacctcc
      901 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
      961 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     1021 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     1081 acagtttgct actagtccat tctcgcaatc cagaacaaac gacccaacac cgtgcgtttt
     1141 attctgtctt tttattgccg tcacacttcg gcggtcagca gctcgttcag ggttttcacg
     1201 tcgtagttgc cgaacactgt ggtggctttc aggttctcaa aagccacttt gccgtagcct
     1261 ctggagccgc ttccgcccag gtagtccagt tccagcagct tcaggccgtc tctgatcacc
     1321 ttgaaatctt cctcgacctg gttctcattc tcatcagtga tctcgtagat cagctcgaag
     1381 tcgaatgttg agttccgaat ggctctctcg atctgtctag gattggcctc ggcggtgatt
     1441 ctatcgatgg tgttctcgaa cttcacctcg gtgtagctcc gcacgcccag agaatccagc
     1501 tcgtcggcgt tgctcaggaa ggcgtcccgg aagatcagtc tgcccatctt gaaccgctta
     1561 tccttgctat ttccgaacag tctgctcagg atgtcgctgt cgtcgctagg cttctcggcc
     1621 accttttcgt tgtacacctt ggccagcagt gttctcatct tgcccttcag gctagagccg
     1681 gggatgatag gcaggttggt gatggggtcc ttgatcacag ggctgtcgat ggcgccgata
     1741 gcggcaaagg catcagatcc tccgatgtgc aggccggttt ccagccggat ctgggcgctg
     1801 aatttgatct tggcgaaggt catgccccgc gccatcttat cgtcatcgtc tttgtaatcc
     1861 atggtggcgg ctctagagat ctgacggttc actaaacgag ctctgcttat atagacctcc
     1921 caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga
     1981 aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt
     2041 ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc
     2101 accgcatgca ttgaaagtaa ggcaaaagaa ctgaacaaac gacccaacac cgtgcgtttt
     2161 attctgtctt tttattgccg tcagtccttg ccgccgtaga acttgaagta ggccaccagg
     2221 gcttccatgt atctgcagaa tctctgcagg gtttcccggt ccttgatctc tttcagagct
     2281 tccaggatct gggccttctc gatcagatct ttcacggcga tctctctgcc ggcctggtac
     2341 acgaactgca ctctcagata agcgattctg tccagcaggg cgtcgtattc cttcaccttg
     2401 ctctcgtcga acagggtgct tgtcaggctc agcaggttcc gcagcttaga tgtggtcagg
     2461 aagaaggcat cagggttctt tctatttctg gtgtttcttt ccagcttcag gatggctttc
     2521 tcggcgatgt ccacgtagtt ctcgtcggtc aggatggtca tgccccgcgc catcttatcg
     2581 tcatcgtctt tgtaatccat ggtggcggct ctagagatct gacggttcac taaacgagct
     2641 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
     2701 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
     2761 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
     2821 tactgccaaa accgcatcac aatgtgtgct gacggctagc taatgtgtgc gaacaaacga
     2881 cccaacaccg tgcgttttat tctgtctttt tattgccgtt aatcctttct gatttcgtag
     2941 atgtacagca ggagcgcgag ctcggcttcc ttccggtcct tatcgttctt gttggtgtac
     3001 caggagtaga acaggttctt gaaggtcttg aacttgtccc tgtcagtctc ccgggtcagt
     3061 tcttccaggc gagtgaggta ataggcgagg cgtgccatat tcatgcggtc gtggttcctc
     3121 aaaagctcaa taagcttata gatgaagttc tttcccctct cgtcttgatg gttgaagaag
     3181 tatctaatct gttccagttt gtcgtcgtac acgttagtga tgaacctatc aaacttgaaa
     3241 gtgtagtcgc ttgagaacag cgagatggag tccttttcgt tgcccttggc ggcctcttcc
     3301 agttccccgg tctggtgagc catcaggcta ataggagtct tgtcggcgaa caaccctatc
     3361 cccgcggaga gggtgagctt cccgttggtc cacttgatga agttttcgcg aagttccaca
     3421 gtgaacgcga tgatatcctg ccacgatcca atggcgaaca cgtcatcgcc gcccgcgtag
     3481 ataatgctca gcttcttgtc ggaggcgaac tggttaatgt acactttgaa gaacagcgac
     3541 atgctccggg agaatgtggc cgatcttgac agagtggagt attgtccgtt tccctgctgg
     3601 ctgaaaccgg ccatgaaggc ggcgcccaag tcatccacgt cgagccggac cacggccagt
     3661 ctcttgatgc ctaggccgtt ctcgttcttg ctcagggcgg cgtagttgta gatctcgtcg
     3721 cactggtaat cccccacgaa cacatgcgta gccttcacgg taccggcctt atagtcattc
     3781 ttcacgtaga cccggctgaa cgcttcttgg gacagctttt cgaatgccac gccctttaag
     3841 cacgcgtttg gtccaatcgg cagcccctca ttttcggtaa tgatgaagtg gtcatgggca
     3901 atttctttcg agaactggta cagtccccgg caaatgtcac agactttctg gtcgtggtag
     3961 gacaccaggt tctccacgga gtggcaaatc tcgcactctc tctctgagga ctttccaccg
     4021 cgattcagga gcatcagtgt ctggtagtcg tatctggaga ttttcttttt ggagatcatg
     4081 cgcgaagcct tttggtacac ttggcggtag gactcggggc tattcagctc ggacatgatg
     4141 tccttggccg cgaaggaacc ccagccaaag gccacataga ggcgggtctg gaagtttgcc
     4201 aacaggaact ggttgaaatc cttctcaaac tgcaccaggg tttccacagt cttttcggtg
     4261 ttggccagga cgaagtaggc gtgtccgccg ccgacgtaaa gcatgttagc cctgttcagt
     4321 cccagcttgt ccagcaggct atcggcgatg tactcggaca taaagtccag gtagaggctc
     4381 cgggccttca gctgcttcgc cgcgccgtta gttgcgatgt taatgttgta gataaagtcc
     4441 tggattcccg acaggtcgaa ggaggccagc aggaaggctt cttcttcata gaacgctgac
     4501 actttggtga acaggtcctc tttgtagttg tgccgaccct tgtcctccag gtagtcgtag
     4561 atcgccagag cgaaggcagc agtcaagcgg gaatggtcgg ccagggagat gtcggcgatt
     4621 tccttagtgt tggtgcttga cggcacgaag gagagagtag cctcgaaaag gttgagcagg
     4681 gagtcaatct ggacttggtt gaactcgaac tcggccagtt cgttcttaat ccgggtggcg
     4741 atggcagcgt aatcgccctt gctaaagggt tcgtaagtgg cggacgcgaa gttgggcttc
     4801 gacttgagat tcagcacggt aggcttgaag taccgcttat cggtctgcgc tccgaacacg
     4861 ttaaagatgt cggcctggtt cgtgtaggtg tcccagatct ttgcggaggt atcttcgtct
     4921 gactcttcgt tggattgccg gcggtcgaca ccggaggcga tgttgtcggc aatgtaggtg
     4981 atgtaagcca ggtgatcgtt gccgagctta tcagactggt agttggccat atggtaccgg
     5041 atctgatccg agatgacttg gttgtcggcg atctcgtcga accagtcggc gcccacaagt
     5101 gcgtgtttct tccgctctcc ggttgctcgc tggatgacct ttccgatgtc gtgcagcagg
     5161 gctccgtaaa acagatcaat cttttctttc ttcatgcccc gcgccatctt atcgtcatcg
     5221 tctttgtaat cgccggaatg gtgatggtga tggtgcatgg tggcggctct agagatctga
     5281 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     5341 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     5401 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     5461 tatccacgcc cattgatgta ctgccaaaac cgcatcacgc ttaagaaaaa ggccgcgttg
     5521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5941 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7261 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     7321 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7381 tgacacatgt caaccggtgc actctcagta caatctgctc gaacaaacga cccaacaccg
     7441 tgcgttttat tctgtctttt tattgccgtc aggcaccggg cttgcgggtc atgcaccagg
     7501 tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc cgctcgtaga
     7561 aggggaggtt gcggggcgcg gaggtttcca ggaaggcggg caccccggcg cgctccgccg
     7621 cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg tcgggcgaga
     7681 ctccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc aggaggcctt
     7741 ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc gggccgatct
     7801 cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc accgcggcgc
     7861 cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg aagagttctt
     7921 gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc gtggcggggt
     7981 agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg cgggtggcga
     8041 ggcgcaccgt gggcttgtac tcggtcatgg tggcggctct agagatctga cggttcacta
     8101 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     8161 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     8221 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     8281 cattgatgta ctgccaaaac cgcatcacat aattcaatgc gggatccctc tatcgcacga
     8341 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcag tcctttcttt
     8401 cttccagctt gatgccgccc attcccaggc tggttttcat gcccaggccg ctgtactcgc
     8461 cgaaggtcag cagcatcttc acgtaggcct tcagtgtctg ggctccctgc accttgaagg
     8521 tcagcttgcc ccggaaggcg gggattctct gtctatgcac tctgaagtag cttgtttcca
     8581 gtctgtaatt ggtgatggtg ctgtgctcgc tcaggtagtc cagggtatct tcctcgatct
     8641 caggctggtt ttccaccagt ctgccgtact tctgcatcag gctctgaaag atcagccgca
     8701 tgctggggaa gatcacgtag ctgccctggc ttttaaagcc tgtaggtgtc tcgaagatga
     8761 tgctgaagta ggtctggttt tcctcgctgt tgaagatctc gagcagctta tcagcgccca
     8821 gctcctggat ttccaccttt tccacagaca acttaggcag gtcgttcagg gacacttcct
     8881 tgatctgcag cagggtcatg aacaccttgt cctcgatgtc gtctgtcagc agatgcacga
     8941 cccactgggt gttctctttg ccctggatca cctttgtggc gtaggggttg gtctgctgct
     9001 ggtgcaggta gtccacgtag tcgctatcca gctgttccat caggaagccg tggaatttca
     9061 cggccaggtc ctgagcaggg tggtcgatcc gcttaaaggt gaacacgagc tttttcatgc
     9121 cccgcgccat cttatcgtca tcgtctttgt aatccatggt ggcggctcta gagatctgac
     9181 ggttcactaa acgagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
     9241 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     9301 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     9361 atccacgccc attgatgtac tgccaaaacc gcatcactta gacttttatt ctccggccgg
     9421 cacacatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat tgccgtcact
     9481 tgtcgatttc cttgatcatg aagttggctt tgcccatctc gtagaagctc tcgtgattct
     9541 tcaccaggct gtggttgccg ctggggatct tcactgtttt cagaggggcc tttgtcagct
     9601 tcagcacgcc cttcttgacc atcttggttt tcattctgga gtatctccgc tgcaggatgc
     9661 cgtcggcctg cttaaacagg gtctttgtcc aagcgccgct gccggcgccc aggtagatgg
     9721 ggtattgcag attagcctgg atcttatcat cagggaattc agacaggaaa aaggccttgt
     9781 agtccttata aaaggcctgg gcccgctttc ccagttcctc gatcagtctg ccggcctcat
     9841 cagtggttgt tgttatctcg aactcgatct tggtcagagg gctgatagac tctctgtaca
     9901 gaggcagagg cttggccttg ttggttttgg cgctgtagtc ccacttctgc acgaggatca
     9961 ggcttttgtt gtcgaagggc ttgctgtcgg acactctgat ggcgttgaac aggtcgtcgt
    10021 attccttgcc tttcttaggt ccccaaggga tcaggttctt gttttccttt gggaatctgc
    10081 cgaagtcgtt cacggcgttc tcgttgttcc acttaggtgt ggtgttcatc aggatggtgc
    10141 ggatggcgcc cttcaggctg ctgcctggga tgtaaggatt tccgaaggcg tcccggatga
    10201 atttgttcac ctcgttgata gctccggcgc tgttagggtt cttatcagat tccaggccgg
    10261 tttcactgat gctgtagccg ccaaaagatc tttcggcgat tctgttgtcg ttcagaaaag
    10321 aaatcagccg gttgtttctg gcgttgggtc tggtctggat caggaaggcc tcgaacttct
    10381 cggccagtct cttttccacc atcttgttgt agaacttgcc catgtcgggg aagtagaact
    10441 tcttgttctc gtagatgaat tctctgctgg tgtacttctc gccgttgccg atgtggatag
    10501 gagccagggt cagcaggctc agcttgaagg tccggtagtc atttttcatg ccccgcgcca
    10561 tcttatcgtc atcgtctttg taatccatgg tggcggctct agagatctga cggttcacta
    10621 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
    10681 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
    10741 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
    10801 cattgatgta ctgccaaaac cgcatcacgc ttgtccaatt gcctgttttt gaatgtgcga
    10861 acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgttag acttcgagct
    10921 taaagaacag cggcttggcg tagttcagca cggcgtgagg gaaatcgaga gggcgcacgt
    10981 ccacgatctg tccctcgaaa gtc
//
