LOCUS       sequence-434408-        2304 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nucleoplasmin NLS for protein targeting to nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Hieronimus K, Donauer T, Klein J, Hinkel B, Spanle JV, Probst A, 
            Niemeyer J, Kibrom S, Kiefer AM, Schneider L, Husemann B, Bischoff 
            E, Mohring S, Bayer N, Klein D, Engels A, Ziehmer BG, Stiebeta J, 
            Moroka P, Schroda M, Deponte M
  TITLE     A Modular Cloning Toolkit for the production of recombinant proteins
            in Leishmania tarentolae.
  JOURNAL   Microb Cell. 2024 Apr 30;11:128-142. doi: 10.15698/mic2024.04.821. 
            eCollection 2024.
  PUBMED    38799406
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Cell."; date: "2024-04-30"; volume: "11:128-142. doi"; pages: " 
            10.15698/mic2024.04.821. eCollection 2024"
COMMENT     Sequence Label: pMBS-844
FEATURES             Location/Qualifiers
     source          1..2304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     416..433
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             515..562
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(604..622)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        690..794
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1016..1807
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(1978..2304,1..262)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
       61 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
      121 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
      181 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
      241 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
      301 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
      361 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
      421 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
      481 gccgattcat taatcactct gtggtctcac catgaaaagg ccggcggcca cgaaaaaggc
      541 cggccaggca aaaaagaaaa agggaatgtg agaccacgaa gtggctcttc agtggacgaa
      601 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      661 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      721 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      781 gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      841 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttttg gggtacagtc
      901 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
      961 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag
     1021 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     1081 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     1141 gaagccacac agcgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     1201 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     1261 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     1321 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     1381 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     1441 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     1501 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     1561 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     1621 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     1681 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     1741 cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg
     1801 caaataactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1861 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1921 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1981 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2041 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2101 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2161 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2221 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2281 gcagcggtcg ggctgaacgg gggg
//
