LOCUS       sequence-434474-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Estradiol-inducible expression of 42-10 Zinc finger activator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Bragdon MDJ, Patel N, Chuang J, Levien E, Bashor CJ, Khalil AS
  TITLE     Cooperative assembly confers regulatory specificity and long-term 
            genetic circuit stability.
  JOURNAL   Cell. 2023 Aug 31;186(18):3810-3825.e18. doi: 
            10.1016/j.cell.2023.07.012. Epub 2023 Aug 7.
  PUBMED    37552983
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.07.012"; journalName: "Cell"; date: 
            "2023-08-31- 31"; volume: "186"; issue: "18"; pages: "3810-3825.e18"
COMMENT     Sequence Label: pKL2-138
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        303..716
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             1933..2166
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     3097..3120
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             3153..3218
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3222..3242
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3243..3476
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             3480..4187
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     gene            4819..6393
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     CDS             5163..6191
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             5163..6191
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      6196..6393
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(6957..7772)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6957..7772)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7680..7699
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7764..7783
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7997..8585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8486..8505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgaactggc cgataattgc agacgaacgc cttgccaaca gggagttctt cagagacatg
       61 gaggctcaaa acgaaattat tgacagccta gacatcaata gtcatacaac agaaagcgac
      121 cacccaactt tggctgataa tagcgtataa acaatgcata ctttgtacgt tcaaaataca
      181 atgcagtaga tatatttatg catattacat ataatacata tcacatagga agcaacaggc
      241 gcgttggact tttaattttc gaggaccgcg aatccttaca tcacacccaa tcccccacaa
      301 gtgatccccc acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt
      361 ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt
      421 tcccctcttt cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa
      481 aaagacaccg cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt
      541 ttctttttct tgaaaatttt tttttttgat ttttttctct ttcgatgacc tcccattgat
      601 atttaagtta ataaacggtc atcaatttct caagtttcag tttcattttt cttgttctat
      661 tacaactttt tttacttctt gctcattaga aagaaagcat agcaatctaa tctaagtttt
      721 aattacaaaa gatctatggg tacccgccca tatgcttgcc ctgtcgagtc ctgcgatcgc
      781 cgcttttctc gctcggatga gcttacccgc catatccgca tccataccgg tcagaagccc
      841 ttccagtgtc gaatctgcat gcgtaacttc agtcgtagtg accaccttac cacccacatc
      901 cgcacccaca caggcgagaa gccttttgcc tgtgacattt gtgggaggaa gtttgccagg
      961 agtgatgaac gcaagaggca taccaaaatc catacaggtg gttctggcac gcccgctgca
     1021 gcgagcactc tagaggaccc tagtgcggga gacatgcgtg ccgcgaactt gtggcctagt
     1081 cccttgatga ttaaacgttc caagaagaac agcttagcgt tgagtcttac ggctgaccag
     1141 atggtcagcg ctctactaga cgctgaaccc ccgatcttat acagtgagta cgatcctact
     1201 cgtccctttt ctgaggcctc catgatggga cttttgacaa acttggcaga tcgtgaactt
     1261 gttcacatga tcaactgggc gaaaagggtg cccggcttcg tagacttgac tctgcacgac
     1321 caggtgcatt tgctggagtg tgcgtggcta gagattctta tgatcggact ggtctggcgt
     1381 agcatggaac atcccgttaa gctgcttttc gctccaaatc tacttttaga taggaaccag
     1441 ggaaaatgcg ttgaaggcat ggtggagata tttgacatgc tgttggcgac ctcttcccgt
     1501 tttaggatga tgaatctaca aggtgaagag tttgtatgtc taaaaagtat aatactgttg
     1561 aatagtggag tctacacatt cttatccagc accttaaagt ctctagagga gaaagaccac
     1621 atacaccgtg ttctagacaa aatcaccgat acattaattc accttatggc taaggctggg
     1681 cttacgctac aacaacagca ccaaagatta gcgcaactat tactaattct gtctcacatc
     1741 agacacatgt caaacaaagg tatggaacac ctttattcta tgaaatgcaa aaacgttgta
     1801 ccgttgtacg atctactgct tgagatgcta gacgctcaca gacttcacgc tccaacaagt
     1861 aggggagggg cttccgtaga agagactgac cagtcacatt tggcgacggc tggtagcacc
     1921 tcctcgggat ccgccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc
     1981 gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg
     2041 gacggggatt ccccggggcc gggatttacc ccccacgact ccgcccccta cggcgctctg
     2101 gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac
     2161 ggtgggtaac tcgagtggca gtgcttttaa ctaagaatta ttagtctttt ctgcttattt
     2221 tttcatcata gtttagaaca ctttatatta acgaatagtt tatgaatcta tttaggttta
     2281 aaaattgata cagttttata agttactttt tcaaagactc gtgctgtcta ttgcataatg
     2341 cactggaagg ggaaaaaaaa ggtgcacacg cgtggctttt tcttgaattt gcagtttgaa
     2401 aaatgctgcc aaaccagatg tcaacacagc tacaacgggt accgggccct tatattgaat
     2461 tttcaaaaat tcttactttt tttttggatg gacgcaaaga agtttaataa tcatattaca
     2521 tggcattacc accatataca tatccatatc taatcttact tatatgttgt ggaaatgtaa
     2581 agagccccat tatcttagcc taaaaaaacc ttctctttgg aactttcagt aatacgctta
     2641 actgctcatt gctatattga agtgaattcg gcgtgggcgt gcgtgggcgg gcgtgggcgt
     2701 gcgtgggcgg gcgtgggcgt gcgtgggcgg gatccccgtg cgtcctcgtc ttcaccggtc
     2761 gcgttcctga aacgcagatg tgcctaatgc cgcactgctc cgaacaataa agattctaca
     2821 atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc cacaaacctt
     2881 caaattaacg aatcaaatta acaaccatag gatgataatg cgattagttt tttagcctta
     2941 tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga tatataaatg
     3001 gaaaagctgc ataaccactt taactaatac tttcaacatt ttcagtttgt attacttctt
     3061 attcaaatgt cataaaagta tcaacaaaaa attgttaata tacctctata ctttaacgtc
     3121 aaggagaaaa aactatactc gagagatcta tggactacaa agaccatgac ggtgattata
     3181 aagatcatga catcgattac aaggatgacg atgacaaggg ccctaaaaag aagcgtaaag
     3241 tcgccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg
     3301 cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt
     3361 ccccggggcc gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg
     3421 acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggggcg
     3481 tgtccaaagg agaggagtta atcaaggaaa acatgagaat gaaagttgtc atggagggct
     3541 ccgttaatgg tcaccaattc aagtgtacag gggaaggtga aggtaatcct tacatgggta
     3601 cacaaactat gagaattaaa gtaattgaag gcggaccact accatttgca tttgacattc
     3661 tggcaacgtc attcatgtac ggatcacgaa ctttcatcaa gtaccctaaa ggtataccag
     3721 actttttcaa gcaatctttt ccagagggtt ttacatggga aagggttaca agatacgaag
     3781 atgggggtgt cgtcacagtt atgcaagata cttcattaga agatggctgc cttgtctatc
     3841 atgtgcaagt aagaggggtg aattttcctt ctaacggacc tgtgatgcag aaaaagacca
     3901 aaggttggga accaaatact gaaatgatgt acccagctga tggaggtttg agaggctaca
     3961 cacacatggc gcttaaagtt gatggtggag gtcatttgtc ttgtagtttt gttaccactt
     4021 atcgttctaa aaagactgtt ggcaatatca aaatgccagg aatacatgct gtagaccaca
     4081 gactagaaag actcgaagag agcgataacg aaatgttcgt tgtacagaga gagcatgccg
     4141 tagccaaatt tgctggctta ggcggtggta tggatgaatt gtataagggt tcttctagac
     4201 ccggggaggc ccccttccag tgtcgcattt gcatggcgaa cttttcgacc ggccagatcc
     4261 ttgaccgcca tacccgtact cataccggtg aaaaaccgtt tcagtgtcgg atctgtatgg
     4321 caaatttctc cgtggcgcac agcttgaaga ggcatctacg tacgcacacc ggcgagaagc
     4381 cattccaatg ccgaatatgc atggccaact tcagtgaccc cagcaacctg cggcgccacc
     4441 taaaaaccca cctgagagga tccggctccg gatctgtcaa agagtccctt gtgtaactcg
     4501 agtggcgcca attggtgcgg caattgataa taacgaaaat gtcttttaat gatctgggta
     4561 taatgaggaa ttttccgaac gtttttactt tatatatata tatacatgta acatatattc
     4621 tatacgctat agagaaagga aatttttcaa ttaaaaaaaa aatagagaaa gagtttcact
     4681 tcttgattat cgctaacact aatggttgaa gtactgctac tttaatttta tgctgagcag
     4741 ttacagagat gttacgaacc actagtgcac tgcagtacag tttagcttgc ctcgtccccg
     4801 ccgggtcacc cggccagcga catggaggcc cagaataccc tccttgacag tcttgacgtg
     4861 cgcagctcag gggcatgatg tgactgtcgc ccgtacattt agcccataca tccccatgta
     4921 taatcatttg catccataca ttttgatggc cgcacggcgc gaagcaaaaa ttacggctcc
     4981 tcgctccaga cctgcgagca gggaaacgct cccctcacag acgcgttgaa ttgtccccac
     5041 gccgcgcccc tgtagagaaa tataaaaggt taggatttgc cactgaggtt cttctttcat
     5101 atacttcctt ttaaaatctt gctaggatac agttctcaca tcacatccga acataaacaa
     5161 aaatgggtaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     5221 tcgacagcgt gtccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     5281 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     5341 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     5401 acattgggga atttagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     5461 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcaaccggtc gcggaggcca
     5521 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     5581 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     5641 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     5701 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     5761 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     5821 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     5881 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     5941 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     6001 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     6061 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     6121 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     6181 caaaggaata aagtaactga caataaaaag attcttgttt tcaagaactt gtcatttgta
     6241 tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt
     6301 tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc
     6361 gtcaatcgta tgtgaatgct ggtcgctata ctggagttat cgtgttgcat ctgcggcttt
     6421 aaattgatgt atctcatcgc aggcacgggc agtacagtgc cctgagcgta gggaaaaatg
     6481 aaaaaaagga tgtaactttt aacataattc cagcacgcag cgattgggta taatgaagat
     6541 tgttaagttc aacaacatgt gaaattctta gctccgcaac ttgcttgaat tatgagctct
     6601 aagattcaag aagtaaatag gtcatataat ggaatgatag tagtacttga taaaggaacc
     6661 atgtgatctt acgttgatat gaaaataatt ctctcacaga agtcagtttg tatgactaac
     6721 atagagagta attgcttttc ggataaaatc gcacctaaca aagattacct tttgtaaagc
     6781 ctccagaaca gctatgaatg ttcgttattc tacgcacttt ctttcataca tcttgatccg
     6841 ccttttttga tgtaggtttt aacgcggcaa aacatattaa tttttctgat ttggaaaaat
     6901 aaagagttag ttctgccccg agcggccgcg tgttacaacc aattaaccaa ttctgattag
     6961 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     7021 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     7081 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     7141 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     7201 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     7261 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     7321 tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     7381 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     7441 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     7501 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     7561 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     7621 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     7681 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctg
     7741 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     7801 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     7861 ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag
     7921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc gccaattcta
     8701 attgtatcga gatcactttt cgtgatccgc taatcagcga cggtcacatt aggtttgcca
     8761 agtcagggta tgaaccatac gatcagtttt cgtgaacctg gtacgtatat tgtggcgttt
     8821 gtgtatattt tcattctttg acaacaatca ataccaacct caaataggaa aagtaataag
     8881 tttggcgtta caccccaaaa gacgccaaac ggatcgaact tactcaatag caattagcga
     8941 gacaaaacct acgttaagac ctgtaaccga tttatcaaag cactctgcgg ttctttcttg
     9001 ggaatattac ctggacattt tgtgccctca agaaacgagg ctctacgagc ctgttggagc
     9061 ccctcagaca ttagccgcca cgaatcaaac tttttacgcg attcggccca aatcagtttc
     9121 tcacagatca ttcgtagagt gaaaaagcac atcgattatt tgatacccct ttgggttaat
     9181 tactgttgag gtctttgccg taagttccat cgctgtccct gaattcgcat ctaga
//
