LOCUS       sequence-434475-        7528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear expression of Synrophin PDZ clamp protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7528)
  AUTHORS   Bragdon MDJ, Patel N, Chuang J, Levien E, Bashor CJ, Khalil AS
  TITLE     Cooperative assembly confers regulatory specificity and long-term 
            genetic circuit stability.
  JOURNAL   Cell. 2023 Aug 31;186(18):3810-3825.e18. doi: 
            10.1016/j.cell.2023.07.012. Epub 2023 Aug 7.
  PUBMED    37552983
REFERENCE   2  (bases 1 to 7528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.07.012"; journalName: "Cell"; date: 
            "2023-08-31- 31"; volume: "186"; issue: "18"; pages: "3810-3825.e18"
COMMENT     Sequence Label: pKL1-222
FEATURES             Location/Qualifiers
     source          1..7528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        278..691
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             720..740
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2073..2260
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2128..2152)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(4906..5697,5698..5766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5529..5548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5767..5871)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5974..6562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6463..6482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        6912..7319
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
ORIGIN
        1 aacgccttgc caacagggag ttcttcagag acatggaggc tcaaaacgaa attattgaca
       61 gcctagacat caatagtcat acaacagaaa gcgaccaccc aactttggct gataatagcg
      121 tataaacaat gcatactttg tacgttcaaa atacaatgca gtagatatat ttatgcatat
      181 tacatataat acatatcaca taggaagcaa caggcgcgtt ggacttttaa ttttcgagga
      241 ccgcgaatcc ttacatcaca cccaatcccc cacaagtgat cccccacaca ccatagcttc
      301 aaaatgtttc tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta
      361 ccacttcaaa acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt
      421 cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga caccgcctcg tttctttttc
      481 ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt
      541 ttgatttttt tctctttcga tgacctccca ttgatattta agttaataaa cggtcatcaa
      601 tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca
      661 ttagaaagaa agcatagcaa tctaatctaa gttttaatta caaaagatct atgctcgagc
      721 caaaaaagaa aaggaaagta gtagaactac agcgtcgtag ggtaacagta aggaaggccg
      781 atgcaggcgg ccttggcatc agtataaaag gtgggagaga aaataagatg cctattttaa
      841 tatcaaagat cttcaaaggt ttagcagcgg atcagacaga ggcccttttc gttggggatg
      901 ctatcttaag tgtcaacggc gaggatcttt cttcagcaac ccatgatgag gccgtgcaag
      961 cactaaaaaa gacaggaaag gaagtagtat tagaagtaaa atatatgaag gaggtaagcc
     1021 cctattttaa gggcagcgga gggagttcag ggagcggtcc tggcagtagt ggcggaagcg
     1081 gtcctggtct acaacgtcgt agagtcacgg ttcgtaaggc tgacgcgggt ggcctgggaa
     1141 ttagtataaa aggcggaaga gaaaataaaa tgcctatact gatctcaaaa atctttaagg
     1201 gtttggcagc ggatcagact gaagcacttt ttgttggcga cgccatactt agcgtgaatg
     1261 gggaagactt gtctagtgca acgcatgacg aggcagtcca agcactgaaa aagactggca
     1321 aagaggtggt actggaggta aagtatatga aggaggtttc tccatacttt aaaggttcta
     1381 gcggcggaag tggcagcggg ccaggatcag ggggatcatc tggacccggt ctacagagaa
     1441 ggcgtgttac ggtacgtaaa gctgatgccg gtggcttagg gatctcaata aagggtggca
     1501 gagaaaacaa aatgcccata cttattagta agatatttaa gggccttgcc gcagatcaga
     1561 ccgaggccct ttttgttggt gatgctatct tgtcagtcaa tggggaagat cttagtagcg
     1621 caacccatga cgaggccgta caagcattga agaaaaccgg aaaagaagta gttttagaag
     1681 tcaaatatat gaaggaggtt agtccgtatt tcaagggggg ttccagcggc agcgggggat
     1741 cccccgggtc tggtagcggc gggtcacccg gcctgcaaag gaggagggtt acggttagaa
     1801 aggcggacgc gggcggttta ggcatctcta tcaagggagg gcgtgagaat aaaatgccca
     1861 ttttgatatc aaagatattt aaaggcctgg cggccgacca gacggaggcg ctgtttgttg
     1921 gcgacgctat tctaagcgta aatggcgagg atcttagctc cgctacacat gatgaagctg
     1981 tacaagctct gaaaaaaacc gggaaagaag tggttctgga agtgaaatat atgaaggagg
     2041 ttagccccta cttcaaggga tcctaactcg aggcgaattt cttatgattt atgattttta
     2101 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     2161 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     2221 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgccgagc aaatgcctgc
     2281 aaatcgctcc ccatttcgct gccaatgaga cgacggggtc atcacggctc atcatgcgcc
     2341 aaacaaatgt gtgcaataca cgctcggatg actgcatgat gaccgcactg actggggaca
     2401 gcagatccac ctaagcctgt gagagaagca gacacccgac agatcaaggc agttaactag
     2461 tgcactgcag tacattacca atagatagcg gtttaatata aggtttcaac aagaaatatt
     2521 ttgtgaaaat caaaagttaa tagtttcact gtcgaaaata tgaaagttta agttttcagt
     2581 tcatcccatt gtagttatca aatttctgtg tttcccgtat atgtgtgctc tgtattcatc
     2641 gctatcttga aactggttat gatcgttgcg catgtgacct gtatcgctca aaagagcccg
     2701 gcaccggtac cgggcaaaag cccctagcaa gctaaaatga ttcaactttc gtagtaattt
     2761 gttcaaccgg tcacagtaaa gtcaatgact aacttttgaa tcgaggatgt atttccaatt
     2821 gaaagcaatg tatatataaa ccattgtgtt gaagcttgtc ttacagtttt cttgtatcct
     2881 cccattgttc atatagtact taaagtacca taatccttaa agaacaaact tgaaagagaa
     2941 tacactatac acaactaata aataaagaat aatcatggct gtgaccaaga caattgtagt
     3001 tctaccaggt gaccatgttg gtcaagaaat cactgaagag gccattaaag ttttaaatgc
     3061 tattcaggaa tgtcgtccag acaaggtcaa tttcaagttt gatcatcatt tgatcggtgg
     3121 tgctgcaatt gatgccactg gtgttccatt accagacgaa gctttggagg cctctaagaa
     3181 agctgatgcc gtgcttctgg gtgctgttgg tggtccaaaa tggggtactg gcgctgtcag
     3241 accagaacaa ggtcttttga aaatccgtaa ggagttgcaa ttgtatgcta atctaagacc
     3301 atgtaatttt gcatctgatt ccttactaga tctatcgcca ttgaagcctg aaattgcaag
     3361 aggtacagat ttcgtcgttg ttagagaact agtgggtggt atttatttcg gggagagaaa
     3421 agaagatgaa ggtgatggtg tcgcctggga tagcgaaaag tattctgtgc ccgaagttca
     3481 aagaatcact agaatggctg ctttcatggc tctgcaacac aacccacctt tacctatttg
     3541 gtccctagat aaagccaacg ttctagcatc atcaagatta tggagaaaaa cagtggaaga
     3601 aacgattaag aatgaattcc cacaattgac tgttcaacat caattgattg attccgctgc
     3661 tatgatcttg gttaagaacc caactcatct aaatggtatt attatcacaa acaatatgtt
     3721 tggtgatatt atctccgatg aagcctccgt gatcccaggt tcgttaggat tacttccatc
     3781 tgcctccttg gcatctcttc cagataagaa tacggcattt ggtttatatg agccatgtca
     3841 tggctctgct ccagacttac caaagggaaa ggttaacccc gtagcaacaa tcctatctgc
     3901 tgctatgatg ttgaagttgt ctctggacct gttcgaagaa ggtgaaatta tagaacaagc
     3961 agtcaagaaa gtgttggatt ctggtatcag aacagcggat ctaaagggta ccaactctac
     4021 tactgaggtc ggtgatgcag tagcgaaagc tgtcagggaa ctattagctt agacttagta
     4081 gatgtacagt tgcatacata agtctgaaca gaaagaagta aatatcatat ctagttcggt
     4141 ttagaaatca gtgactatat tggttttgac cagatcgcaa tccttgcaag catttagtaa
     4201 tgactatcaa accggtgaat catctgataa ggctatatta gcttgtgcat tcgcatgtat
     4261 cgggaaacga acgagtccga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt
     4321 gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat
     4381 aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagacg aaactatata
     4441 cgcaatctac atacatttat caagaaggag aaaaaggagg atgtaaagga atacaggtaa
     4501 gcaaattgat actaatggct caacgtgata aggaaaaaga attgcacttt aacattaata
     4561 ttgacaagga ggagggcacc acacaaaaag ttaggtgtaa cagaaaatca tgaaactatg
     4621 attcctaatt tatatattgg aggattttct ctaaaaaaaa aaaaatacaa caaataaaaa
     4681 acactcaatg acctgaccat ttgatggagt ttaagtcaat acctccttga accatttccc
     4741 ataatggtga aagttccctc aagaatttta ctctgtcaga aacggcctta acgacgtagc
     4801 cgagcggccg cgattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5041 accgcgggac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5881 ccgaaaagtg ccacctgtca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5941 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6001 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6061 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6121 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6181 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6241 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6301 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6361 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6421 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6481 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6541 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6601 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6661 tgcggccgcc aatgaggaga acttctagta tatctacata cctaatatta ttgccttatt
     6721 aaaaatggaa tcccaacgat tacatcaaaa tccacattct cttcaaaatc aattgtcctg
     6781 tacttccttg ttcatgtgtg ttcaaaaacg ttatatttat aggataatta tactctattt
     6841 ctcaacaagt aattggttgt ttggccgagc ggtctaaggc gcctgattca agaaatatct
     6901 tgaccgcagt taactgtggg aatactcagg tatcgtaaga tgcaagagtt cgaatctctt
     6961 agcaaccatt atttttttcc tcaacataac gagaacacac aggggcgcta tcgcacagaa
     7021 tcaaattcga tgactggaaa ttttttgtta atttcagagg tcgcctgacg catatacctt
     7081 tttcaactga aaaattggga gaaaaaggaa aggtgagagc gccggaaccg gcttttcata
     7141 tagaatagag aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat
     7201 tatttaagga cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt
     7261 ttcttacctt ttacatttca gcaatatata tatatatatt tcaaggatat accattctaa
     7321 tgtctgcccc taagccctga attcgcatct agatggtaga gccacaaaca gccggtacaa
     7381 gcaacgatct ccaggaccat ctgaatcatg cgcggatgac acgaactcac gacggcgatc
     7441 acagacatta acccacagta cagacactgc gacaacgtgg caattcgtcg caataccgtc
     7501 tcactgaact ggccgataat tgcagacg
//
