LOCUS       sequence-43449-e       11011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical 
            inhibition..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11011)
  AUTHORS   Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R,
            Gunaydin LA, Hyun M, Fenno LE, Gradinaru V, Yizhar O, Deisseroth K
  TITLE     Principles for applying optogenetic tools derived from direct 
            comparative analysis of microbial opsins.
  JOURNAL   Nat Methods. 2011 Dec 18;9(2):159-72. doi: 10.1038/nmeth.1808.
  PUBMED    22179551
REFERENCE   2  (bases 1 to 11011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-12-18- 18"; volume: "9"; 
            issue: "2"; pages: "159-72"
COMMENT     Sequence Label: pLenti-CaMKIIa-Mac 3.0-EYFP
FEATURES             Location/Qualifiers
     source          1..11011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..133
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(134..202,203..994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(352..371)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1165..1753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1654..1673
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1907..1924
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2041..2062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2077..2082,2083..2100,2101..2107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2115..2131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2120..2142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2139..2155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2139..2155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2204..2325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2215..2234)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2269..2288
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2455..2829)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2848..2895)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2943..3272)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2957..3092
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3011..3030)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3257..3277
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3286..3714)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3396..3417)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3608..3627
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3760..3984
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3973..3990
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             4016..4196
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     4701..4717
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4702..4718
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4721..5309
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4827..4838
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5236..5256
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(5361..6071,6072..6074))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5403..5424)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5752..5771
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6011..6032
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(7122..8410)
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     misc_feature    8455..8572
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8870..8914)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9099..9332
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9825..9950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9997..10177
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(10195..10393)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10220..10240)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10395..10774
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10946..10965
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
       61 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
      181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
      241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
      301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
      361 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
      421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
      481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
      541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
      601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
      661 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
      721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
      781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     1021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     1321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1921 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1981 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     2041 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2101 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2161 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
     2221 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     2281 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
     2341 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     2401 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtagca cgtgtcagtc
     2461 ctgctcctcg gccacgaagt gcacgcagtt gccggccggg tcgcgcaggg cgaactcccg
     2521 cccccacggc tgctcgccga tctcggtcat ggccggcccg gaggcgtccc ggaagttcgt
     2581 ggacacgacc tccgaccact cggcgtacag ctcgtccagg ccgcgcaccc acacccaggc
     2641 cagggtgttg tccggcacca cctggtcctg gaccgcgctg atgaacaggg tcacgtcgtc
     2701 ccggaccaca ccggcgaagt cgtcctccac gaagtcccgg gagaacccga gccggtcggt
     2761 ccagaactcg accgctccgg cgacgtcgcg cgcggtgagc accggaacgg cactggtcaa
     2821 cttggccatg gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga
     2881 tgattaattg tcaacacgtg ctgatcagat ccgaaaatgg atatacaagc tcccgggagc
     2941 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     3001 gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     3061 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     3121 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     3181 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     3241 cctggggact ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt
     3301 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     3361 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     3421 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     3481 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     3541 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     3601 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     3661 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
     3721 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
     3781 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
     3841 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     3901 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     3961 atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctgcta
     4021 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     4081 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     4141 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     4201 cagtccggat gcagctctcg ggccatgtga tgaaatgcta ggcggctgtc aaacctccac
     4261 tctaatactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg taacaagcgg
     4321 gtgttctctc cttcattggc ttcttctacc ttctcttgct caactggtac tagcttgtag
     4381 caccatccaa aggtcagtgg atatctgatc cctggccctg gtgtgtagtt ctgccaatca
     4441 gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct tcgttgggag
     4501 tgaattagcc cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc
     4561 ttgtaagtca ttggtcttaa aggtacctga ggtgtgactg gaaaacccac ctcctcctcc
     4621 tcttgtgctt ctagccaggc acaatcagca ttggtagctg ctgtattgct acttgtgatt
     4681 gctccatgtt tttctaggtc tcgaggtcga cggtatcgat gcggggaggc ggcccaaagg
     4741 gagatccgac tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc
     4801 aggccgcggg aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc
     4861 ccgcgcagaa tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc
     4921 gacaacacca cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg
     4981 caggcggcga tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg
     5041 agctgacagg tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac
     5101 accacgccac gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat
     5161 ttatacaagg aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt
     5221 aaagcagcgt atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt
     5281 tcacaaattt tgtaatccag aggttgatta tcgataagct tgatatcgaa ttctcattac
     5341 acctcgttct cgtagcagaa cttgtacagc tcgtccatgc cgagagtgat cccggcggcg
     5401 gtcacgaact ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg
     5461 gactggtagc tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg
     5521 ttctgctggt agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg
     5581 aagttcacct tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg
     5641 tagttgtact ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc
     5701 agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag
     5761 ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac
     5821 ttgaagaagt cgtgctgctt catgtggtcg gggtagcggg cgaagcactg caggccgtag
     5881 ccgaaggtgg tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac
     5941 ttcagggtca gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg
     6001 tggccgttta cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc
     6061 tcgcccttgc tcaccacgtt gatgtcgatc tggtccaggg ggatgtactc gccctcgctg
     6121 gtgatcctgc tcttggcggc cgccttgctc actgctacta ccggtcgggc gccgtcgtcc
     6181 tcgccgatcc tgatggcgcc ctccctgttc aggccgttgg cccagaagcc gttcagctcc
     6241 acgtcgctct ccctcaggtt ggcgtgggtc accagcagcc aagctccgaa cactccctta
     6301 gccagcacgt ccagcacagc gtaagcgatg atctctccgt ccactccgat cttcctagct
     6361 ccgtcagcca gtccccacac gattgggtaa gcggtccaca ggatcagggt gtaagctccg
     6421 atggccacga agaagctcct cagcttctcg cccttcaccc tggcgttggc gccgccgttc
     6481 agcaccaggt gccacaccac gaagatgtag gcgatgcagg cgatggtgta ccatccccac
     6541 ttctgtgggg ttccctcgct gccgaaagca gcgaacaggc cggtcagcac catgatcagg
     6601 tcagccacga tggccatgaa gatgtgggct ccgctcattc cagccagcag tcccaggtcc
     6661 agcagcagca gtggggtggt gatagcccag tcgatgtacc tggcgtagta cacctgtcgg
     6721 tacacggtct cgtaggtgtc gggcacgtgg tcgtgctggg tcctgatcac gatcttgttc
     6781 agggccactc cgtggccggt agccatagcg aagtagctca gggcggcggt cagggtgatg
     6841 atggtggtga tcacgtggta cagcctcctg ttcacgggga tcttccagct cagggcggtg
     6901 aaggcggcgc tggcgatcag catcagcacg aacaccaccc acagggtctt gctgccgctg
     6961 tcgcccacgg tctcgtagat gggggtgtcg ggcagcacgg ttggcacggg ggccacgtgg
     7021 gttggctggg cgctctgggt agcggttggc agtgggaaca gctggctggt cttcatcagc
     7081 acctcctcga actggtccac gatcatggtg gcggatcccc cgctgccccc agaactaggg
     7141 gccactcgcc tgcccgtgct cctgagtgca aacggagaac cggcttgact gacgagcttg
     7201 gggcttctga gcagggcact gtggctgctc acagcctcgc gagccttcgt cagcatccag
     7261 gtccccatgg caaccacctc cgaaacgccc tgttcccgtt gccccggtaa ctgcctcccc
     7321 aacacctgcc tgcctttcac ttaaaacctg ctcctccgtt gccctggcct tatatacaca
     7381 atactgcagg actgtgtggg cccagaagca agtggacctt gttctcccag ggtgatgtac
     7441 tgagggggtt ggagaagggg atgtgaacaa acttagtcca caagtgatca tcgctcttcc
     7501 tacctccccc acctccaact atagggccct tttaacagac gggatgggat taagtggggc
     7561 aaagaggcag ttgctatggt aacggctagt gggtgccaca ttctgggatg gtccttgaga
     7621 aagacaggat gtagttaccc tggcaacttc atctcctctg aagagacagg tattgccccg
     7681 ttgccctggc aacagctggg ttctaaggtt tacaggggaa gctaattcat ctctccttcc
     7741 cttccccctt gtagtctctg taatggtttc tttgcttcct ctgggaattg gtctggttga
     7801 cttggcctct gtctctgtcc ttgtttcttt ccagctctcc ctgcttttta gccattgtgc
     7861 atatcacacc gccaccttag cgtgagaaga agtaccaaac agacccagaa tgctgacgga
     7921 atgactctgt ctgctttctc acccctatct gtcaccactg agcagcaggg cccctcctga
     7981 ggcctctgcc acagctctcc ccacccatcc tcccagaggg accatgaaga tttggtctcc
     8041 tggggctcct gtcccagttt ctgcttgtgc ttcccgtcaa ggtaggactg gagagaggca
     8101 actataatga ggtgaacgtg gctggactca cccagggctg ggcgggtggt gtagatgtac
     8161 cctgccctgg actcttcttt cctaaactca ttggtcatct gccctaatga gttcactcct
     8221 tctgtatatc gtacttgcat gcccccaagg catggaattt aagattagta atctgagttc
     8281 aagttctagc tctgacattt ctgatgtgac cctgggcagg tcactgagga agctctctgc
     8341 acccccatct cattttctag aaaatcagaa gaagaacccc atggagatga agtgacctaa
     8401 ggccataatg ttaattaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
     8461 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     8521 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     8581 gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa
     8641 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
     8701 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     8761 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     8821 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     8881 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     8941 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
     9001 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
     9061 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
     9121 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
     9181 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
     9241 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
     9301 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
     9361 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
     9421 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
     9481 gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     9541 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     9601 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     9661 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
     9721 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     9781 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     9841 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     9901 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     9961 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
    10021 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
    10081 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
    10141 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt
    10201 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
    10261 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
    10321 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
    10381 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
    10441 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
    10501 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
    10561 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
    10621 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
    10681 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
    10741 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
    10801 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
    10861 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
    10921 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10981 aggggatcgg gagatctccc gatccgtcga c
//
