LOCUS       sequence-434492-        9729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in One IQ-Cumate transgene switch for the EGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9729)
  AUTHORS   Hong J, Sohn KC, Park HW, Jeon H, Ju E, Lee JG, Lee JS, Rho J, Hur 
            GM, Ro H
  TITLE     All-in-one IQ toggle switches with high versatilities for 
            fine-tuning of transgene expression in mammalian cells and tissues.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Feb 2;32(1):101202. doi: 
            10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14.
  PUBMED    38374964
REFERENCE   2  (bases 1 to 9729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-02-2"; volume: "32(1):101202. doi"; 
            pages: " 10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14"
COMMENT     Sequence Label: pSIQmate-EGFP
FEATURES             Location/Qualifiers
     source          1..9729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        568..897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      748..883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             990..1589
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(990..1009)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1486..1506
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1819..1953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1972..2071
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        2240..2619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2620..2823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2774..2794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2868..2886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4687..4743
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4744..5352
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
     CDS             5353..5394
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        complement(5427..5445)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5465..5482)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5471..5695
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    6028..6097
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    6421..6441
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6736..6763
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     promoter        6814..6832
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      6869..6878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6875..6877,6878..6880,6881..7594)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6875..6877,6878..6880,6881..7591)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6920..6941)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7181..7200)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7528..7549
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7681..7815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7854..7953)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(7971..7989)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7994..8010)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7994..8010)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8112..8131)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8123..8932
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8190..8209)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      9079..9667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9568..9587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaact gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      901 cttgcatgcc tgcaggtcgg ccgccacgac cggtgccgcc accatcccct gacccacgcc
      961 cctgacccct cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     1021 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     1081 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     1141 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     1201 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     1261 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     1321 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     1381 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     1441 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     1501 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     1561 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     1621 aaaggagcgc acgaccccat ggctccgacc gaagccaccc ggggcggccc cgccgacccc
     1681 gcacccgccc ccgaggccca ccgactctag aggatcataa tcagccatac cacatttgta
     1741 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1801 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1921 aaactcatca atgtatctta tcatgtctgg atcctcgtta agctcgggcc ccaaataatg
     1981 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     2041 ataatgccaa ctttgtacaa aaaagcaggc tccgataccg ctcgccgcag ccgaacgacc
     2101 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     2161 acgcatctgt gcggtatttc acaccgcata gttaggcgtt ttgcgctgct tcgcgatgta
     2221 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2281 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2341 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2401 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2461 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2521 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2581 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2641 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2701 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2761 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2821 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     2881 tatagggaga cccaagctgc caacatgccg cctaaacgca agacactcaa tgccgctgcc
     2941 gaagccaatg cccacgctga tggccatgct gatggcaatg ctgatggtca tgtcgctaac
     3001 actgcagcaa gcagcaacaa cgcccgtttt gcggacttga ccaacattga cacacccggc
     3061 ctcggcccta ccacgacgac gttacttgtc gagcccgctc gttcgaaacg ccagagagtc
     3121 tcgagggcct gtgatcagtg tcgagctgca cgtgaaaagt gtgatggaat ccagccggct
     3181 tgcttcccct gtgtgtcgca gggccggtcg tgtacctacc aggccagtcc caagaagcga
     3241 ggagtccaga cgggctacat ccgcactctc gaactggctc tggcttggat gttcgagaac
     3301 gttgcccgca gcgaggacgc cctccacaat cttttggtcc gtgatgctgg ccagggcagc
     3361 gctctcctgg tcggcaaaga ctcgcctgct gcagaacgcc tgcatgcaag atgggcgacg
     3421 agtcgagtca acaaaagcat cacccgtctt ctctcaggtc aggccgcaca agatccatct
     3481 gaagacggcc aatccccgtc cgaagacata aatgtccaag atcaggccgc acaagatcca
     3541 tctgaagacg gccaatcccc gtccgaagac ataaatgtcc aagatgagtt cctggatatg
     3601 gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg
     3661 ctgcagatga ggcctgaatg tgtcatacag gagcccagta aaaataaaga caggcaaaga
     3721 caaaagaaag acaaaggaat attattacct gttagtacga ccacagtcga agaccacatg
     3781 cccccgatca tgcaatgtga tccacctccg cccgaggccg ccaggattca cgaagtcgtc
     3841 ccgaggtatc tttcggagaa gctgatggag cagaacaggc agaagaacat accaccattg
     3901 tcggcgaatc agaagtctct gatcgcgagg ctcgtgtggt accaggaggg atatgagcag
     3961 ccctccgacg aggatctcaa aagagtaacg cagacttggc agtcggatga agaggacgag
     4021 gaatccgatc tacccttccg ccagatcacg gagatgacga tcttaacggt ccagttgatc
     4081 gtcgagttcg ccaagggtct accgggcttt tcgaagatat cacagtctga tcaaatcacc
     4141 ttattaaaag cctcgtccag cgaggtgatg atgctgcggg tggcgaggcg atacgacgcc
     4201 gcgtccgaca gcattctgtt cgccaacaac aaggcgtaca cgcgcgacaa ctaccgcaag
     4261 gcgggcatgg ccgaggtcat cgaagacctc ctacacttct gccggtgcat gttcgcgatg
     4321 ggcatggaca atgtgcactt tgcactgctc acggccatcg ttatattctc agatcggccc
     4381 gggctcgagc agccgtcgct ggtagaagag atccagagat actacctgaa cacgttgcga
     4441 atttacatca tcaaccagaa cagcgcgtcg tcgcgctgcg ccgtgatcta cggcaggatc
     4501 ctgagcgtgc tgaccgagct acgcacgctc ggcacgcaaa actccaacat gtgcatctcg
     4561 ctgaagctga agaacaggaa gctgccgccg ttcctcgagg agatctggga cgtggcggag
     4621 gtggccacga cgcatcccac ggtgctgccg cccaccaacc cggtggtgct agcatgcgga
     4681 agcggagcta ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga
     4741 cctatgtcac ctaagagaag aactcaggca gagagagcaa tggaaactca ggggaaactc
     4801 atcgcagccg cactcggggt cctcagagaa aagggctacg caggattccg gatcgccgat
     4861 gtgccaggag cagctggtgt ctcacgcggg gctcagagcc accatttccc taccaaactg
     4921 gagctgctcc tggcaacatt tgagtggctg tatgaacaga tcactgagag gagcagagcc
     4981 cggctcgcta agctgaaacc agaggacgat gtgattcagc agatgctcga cgatgcagcc
     5041 gaattctttc tggacgatga cttcagcatc tccctcgatc tgattgtggc tgcagatcgg
     5101 gaccccgccc tgcgagaagg gattcagcga accgtggagc gcaaccgatt tgtggtcgaa
     5161 gatatgtggc tcggcgtgct ggtgtctagg ggactgagta gagatgacgc cgaggacatc
     5221 ctctggctga ttttcaattc cgtgaggggc ctcgctgtca gatctctgtg gcagaaggac
     5281 aaagagcggt ttgaacgagt cagaaacagc acactggaaa tcgcacggga gagatacgct
     5341 aagttcaaaa ggggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtaaccg
     5401 gtggtgctat agctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     5461 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     5521 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     5581 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     5641 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     5701 gaggcggaaa gttgagagga cattccaatc ataggctgcc catccaccct ctgtgtcctc
     5761 ctgttaatta ggtcacttaa caaaaaggaa attgggtagg ggtttttcac agaccgcttt
     5821 ctaagggtaa ttttaaaata tctgggaagt cccttccact gctgtgttcc agaagtgttg
     5881 gtaaacagcc cacaaatgtc aacagcagaa acatacaagc tgtcagcttt gcacaagggc
     5941 ccaacaccct gctcatcaag aagcactgtg gttgctgtgt tagtaatgtg caaaacagga
     6001 ggcacatttt ccccacctgt gtaggttctc gaagccgcgg tgcgggtgcc agggcgtgcc
     6061 cttgggctcc ccgggcgcgt actccacctc acccatctat ccaaaacagc cttgtggtca
     6121 gtgttcatct gctgactgtc aactgtagca ttttttgggg ttacagtttg agcaggatat
     6181 ttggtcctgt agtttgctaa cacaccctgc agctccaaag gttccccacc aacagcaaaa
     6241 aaatgaaaat ttgacccttg aatgggtttt ccagcaccat tttcatgagt tttttgtgtc
     6301 cctgaatgca agtttaacat agcagttacc ccaataacct cagttttaac agtaacagct
     6361 tcccacatca aaatatttcc acaggttaag tcctcattta aattaggcaa aggaattgct
     6421 caactttgta tagaaaagtt ggggtaatcg cttatcctcg gataaacaat tatcctcacg
     6481 ggtaatcgct tatccgctcg ggtaatcgct tatcctcggg taatcgctta tcctcgggta
     6541 atcgcttatc ctcggataaa caattatcct cacgggtaat cgcttatccg ctcgggtaat
     6601 cgcttatcct cggataaaca attatcctca cgggtaatcg cttatccgct cgggtaatcg
     6661 cttatcctcg ggtaatcgct tatccttgca agggtcgact ctagagggta tataatggat
     6721 cccatcgcgt ctcagaacaa acagacaatc tggtctgttt gtacctcact ttgagctcct
     6781 ccacacgaat tccctcgacc tcgaagaagc ttgatttagg tgacactata gaatacaagc
     6841 tacttgttct ttttgcagga tcccatcggc caccatggtg agcaagggcg aggagctgtt
     6901 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     6961 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     7021 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     7081 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     7141 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     7201 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     7261 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     7321 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     7381 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     7441 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     7501 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     7561 gatcactctc ggcatggacg agctgtacaa gtaacgattc cggacttaag gcggccgcag
     7621 tactcgattc gaattcaagg cctctcgagc ctctagaact atagtgagtc gtattacgta
     7681 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     7741 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     7801 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggg ccgacccagc
     7861 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     7921 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     7981 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     8041 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
     8101 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     8161 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     8221 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     8281 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     8341 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     8401 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     8461 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     8521 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     8581 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     8641 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     8701 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     8761 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     8821 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     8881 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     8941 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     9001 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     9061 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9121 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9181 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9241 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9301 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9361 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9421 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9481 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9541 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9601 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9661 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9721 tcacatgtt
//
