LOCUS       sequence-434495-        8935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in One IQ transgene switch for EGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Hong J, Sohn KC, Park HW, Jeon H, Ju E, Lee JG, Lee JS, Rho J, Hur 
            GM, Ro H
  TITLE     All-in-one IQ toggle switches with high versatilities for 
            fine-tuning of transgene expression in mammalian cells and tissues.
  JOURNAL   Mol Ther Methods Clin Dev. 2024 Feb 2;32(1):101202. doi: 
            10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14.
  PUBMED    38374964
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2024-02-2"; volume: "32(1):101202. doi"; 
            pages: " 10.1016/j.omtm.2024.101202. eCollection 2024 Mar 14"
COMMENT     Sequence Label: pSIQ-EGFP
FEATURES             Location/Qualifiers
     source          1..8935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(563..583)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        568..897
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      748..883
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             990..1589
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(990..1009)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1486..1506
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1819..1953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    1972..2071
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     enhancer        2240..2619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2620..2823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2774..2794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        2868..2886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4689..4707)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4727..4744)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4733..4957
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    5290..5359
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    5683..5703
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      6046..6055
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6052..6054,6055..6057,6058..6771)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6052..6054,6055..6057,6058..6768)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6097..6118)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6358..6377)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6705..6726
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        6778..6796
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    6887..7021
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7060..7159)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(7177..7195)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7200..7216)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7200..7216)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7318..7337)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7329..8138
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7396..7415)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8285..8873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8774..8793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaact gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      601 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      661 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      721 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      781 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      841 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      901 cttgcatgcc tgcaggtcgg ccgccacgac cggtgccgcc accatcccct gacccacgcc
      961 cctgacccct cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg
     1021 ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc
     1081 ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag
     1141 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     1201 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     1261 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     1321 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     1381 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     1441 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     1501 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     1561 ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg
     1621 aaaggagcgc acgaccccat ggctccgacc gaagccaccc ggggcggccc cgccgacccc
     1681 gcacccgccc ccgaggccca ccgactctag aggatcataa tcagccatac cacatttgta
     1741 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1801 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1921 aaactcatca atgtatctta tcatgtctgg atcctcgtta agctcgggcc ccaaataatg
     1981 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     2041 ataatgccaa ctttgtacaa aaaagcaggc tccgataccg ctcgccgcag ccgaacgacc
     2101 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     2161 acgcatctgt gcggtatttc acaccgcata gttaggcgtt ttgcgctgct tcgcgatgta
     2221 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     2281 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     2341 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2401 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     2461 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2521 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2581 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2641 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     2701 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     2761 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     2821 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     2881 tatagggaga cccaagctgc caacatgccg cctaaacgca agacactcaa tgccgctgcc
     2941 gaagccaatg cccacgctga tggccatgct gatggcaatg ctgatggtca tgtcgctaac
     3001 actgcagcaa gcagcaacaa cgcccgtttt gcggacttga ccaacattga cacacccggc
     3061 ctcggcccta ccacgacgac gttacttgtc gagcccgctc gttcgaaacg ccagagagtc
     3121 tcgagggcct gtgatcagtg tcgagctgca cgtgaaaagt gtgatggaat ccagccggct
     3181 tgcttcccct gtgtgtcgca gggccggtcg tgtacctacc aggccagtcc caagaagcga
     3241 ggagtccaga cgggctacat ccgcactctc gaactggctc tggcttggat gttcgagaac
     3301 gttgcccgca gcgaggacgc cctccacaat cttttggtcc gtgatgctgg ccagggcagc
     3361 gctctcctgg tcggcaaaga ctcgcctgct gcagaacgcc tgcatgcaag atgggcgacg
     3421 agtcgagtca acaaaagcat cacccgtctt ctctcaggtc aggccgcaca agatccatct
     3481 gaagacggcc aatccccgtc cgaagacata aatgtccaag atcaggccgc acaagatcca
     3541 tctgaagacg gccaatcccc gtccgaagac ataaatgtcc aagatgagtt cctggatatg
     3601 gccgacttcg agtttgagca gatgtttacc gatgcccttg gaattgacga gtacggtggg
     3661 ctgcagatga ggcctgaatg tgtcatacag gagcccagta aaaataaaga caggcaaaga
     3721 caaaagaaag acaaaggaat attattacct gttagtacga ccacagtcga agaccacatg
     3781 cccccgatca tgcaatgtga tccacctccg cccgaggccg ccaggattca cgaagtcgtc
     3841 ccgaggtatc tttcggagaa gctgatggag cagaacaggc agaagaacat accaccattg
     3901 tcggcgaatc agaagtctct gatcgcgagg ctcgtgtggt accaggaggg atatgagcag
     3961 ccctccgacg aggatctcaa aagagtaacg cagacttggc agtcggatga agaggacgag
     4021 gaatccgatc tacccttccg ccagatcacg gagatgacga tcttaacggt ccagttgatc
     4081 gtcgagttcg ccaagggtct accgggcttt tcgaagatat cacagtctga tcaaatcacc
     4141 ttattaaaag cctcgtccag cgaggtgatg atgctgcggg tggcgaggcg atacgacgcc
     4201 gcgtccgaca gcattctgtt cgccaacaac aaggcgtaca cgcgcgacaa ctaccgcaag
     4261 gcgggcatgg ccgaggtcat cgaagacctc ctacacttct gccggtgcat gttcgcgatg
     4321 ggcatggaca atgtgcactt tgcactgctc acggccatcg ttatattctc agatcggccc
     4381 gggctcgagc agccgtcgct ggtagaagag atccagagat actacctgaa cacgttgcga
     4441 atttacatca tcaaccagaa cagcgcgtcg tcgcgctgcg ccgtgatcta cggcaggatc
     4501 ctgagcgtgc tgaccgagct acgcacgctc ggcacgcaaa actccaacat gtgcatctcg
     4561 ctgaagctga agaacaggaa gctgccgccg ttcctcgagg agatctggga cgtggcggag
     4621 gtggccacga cgcatcccac ggtgctgccg cccaccaacc cggtggtgct atagctagag
     4681 ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct
     4741 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     4801 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     4861 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     4921 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagttgagag
     4981 gacattccaa tcataggctg cccatccacc ctctgtgtcc tcctgttaat taggtcactt
     5041 aacaaaaagg aaattgggta ggggtttttc acagaccgct ttctaagggt aattttaaaa
     5101 tatctgggaa gtcccttcca ctgctgtgtt ccagaagtgt tggtaaacag cccacaaatg
     5161 tcaacagcag aaacatacaa gctgtcagct ttgcacaagg gcccaacacc ctgctcatca
     5221 agaagcactg tggttgctgt gttagtaatg tgcaaaacag gaggcacatt ttccccacct
     5281 gtgtaggttc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     5341 gtactccacc tcacccatct atccaaaaca gccttgtggt cagtgttcat ctgctgactg
     5401 tcaactgtag cattttttgg ggttacagtt tgagcaggat atttggtcct gtagtttgct
     5461 aacacaccct gcagctccaa aggttcccca ccaacagcaa aaaaatgaaa atttgaccct
     5521 tgaatgggtt ttccagcacc attttcatga gttttttgtg tccctgaatg caagtttaac
     5581 atagcagtta ccccaataac ctcagtttta acagtaacag cttcccacat caaaatattt
     5641 ccacaggtta agtcctcatt taaattaggc aaaggaattg ctcaactttg tatagaaaag
     5701 ttggggtaat cgcttatcct cggataaaca attatcctca cgggtaatcg cttatccgct
     5761 cgggtaatcg cttatcctcg ggtaatcgct tatcctcggg taatcgctta tcctcggata
     5821 aacaattatc ctcacgggta atcgcttatc cgctcgggta atcgcttatc ctcggataaa
     5881 caattatcct cacgggtaat cgcttatccg ctcgggtaat cgcttatcct cgggtaatcg
     5941 cttatccttg caagggtcga ctctagaggg tatataatgg atcccatcgc gtctcagcct
     6001 cactttgagc tcctccacac gaattccctc gacctcgaag aagctgccac catggtgagc
     6061 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     6121 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     6181 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     6241 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     6301 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     6361 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     6421 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     6481 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     6541 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     6601 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     6661 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     6721 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcttgatt
     6781 taggtgacac tatagaatac aagctacttg ttctttttgc aggatcccat cgattcgaat
     6841 tcaaggcctc tcgagcctct agaactatag tgagtcgtat tacgtagatc cagacatgat
     6901 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6961 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     7021 taacaacaac aattgcattc attttatgtt tcagggccga cccagctttc ttgtacaaag
     7081 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     7141 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     7201 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     7261 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     7321 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     7381 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     7441 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     7501 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     7561 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     7621 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     7681 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     7741 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     7801 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     7861 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     7921 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     7981 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     8041 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     8101 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     8161 aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc
     8221 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     8281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     8341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     8401 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     8461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     8521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8881 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
