LOCUS       sequence-434524-        5199 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of iCre(R297T) in striatal cholinergic neurons .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Hunker AC, Wirthlin ME, Gill G, Johansen NJ, Hooper M, Omstead V, 
            Vargas S, Lerma MN, Taskin N, Weed N, Laird WD, Bishaw YM, Bendrick 
            JL, Gore BB, Ben-Simon Y, Opitz-Araya X, Martinez RA, Way SW, 
            Thyagarajan B, Otto S, Sanchez REA, Alexander JR, Amaya A, Amster A,
            Arbuckle J, Ayala A, Baker PM, Barcelli T, Barta S, Bertagnolli D, 
            Bielstein C, Bishwakarma P, Bowlus J, Boyer G, Brouner K, Casian B, 
            Casper T, Chakka AB, Chakrabarty R, Chong P, Clark M, Colbert K, 
            Daniel S, Dawe T, Departee M, DiValentin P, Donadio NP, Dotson NI, 
            Dwivedi D, Egdorf T, Fliss T, Gary A, Goldy J, Grasso C, Groce EL, 
            Gudsnuk K, Han W, Haradon Z, Hastings S, Helback O, Ho WV, Huang C, 
            Johnson T, Jones DL, Juneau Z, Kenney J, Leibly M, Li S, Liang E, 
            Loeffler H, Lusk NA, Madigan Z, Malloy J, Malone J, McCue R, Melchor
            J, Mich JK, Moosman S, Morin E, Naidoo R, Newman D, Ngo K, Nguyen K,
            Oster AL, Ouellette B, Oyama AA, Pena N, Pham T, Phillips E, Pom C, 
            Potekhina L, Ransford S, Ray PL, Reding M, Rette DF, Reynoldson C, 
            Rimorin C, Sigler AR, Rocha DB, Ronellenfitch K, Ruiz A, Sawyer L, 
            Sevigny JP, Shapovalova NV, Shepard N, Shulga L, Soliman S, Staats 
            B, Taormina MJ, Tieu M, Wang Y, Wilkes J, Wood T, Zhou T, Williford 
            A, Dee N, Mollenkopf T, Ng L, Esposito L, Kalmbach BE, Yao S, Ariza 
            J, Collman F, Mufti S, Smith K, Waters J, Ersing I, Patrick M, Zeng 
            H, Lein ES, Kojima Y, Horwitz G, Owen SF, Levi BP, Daigle TL, Tasic 
            B, Bakken TE, Ting JT
  TITLE     Enhancer AAV toolbox for accessing and perturbing striatal cell 
            types and circuits.
  JOURNAL   Neuron. 2025 May 21;113(10):1507-1524.e17. doi: 
            10.1016/j.neuron.2025.04.035.
  PUBMED    40403704
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2025.04"; journalName: "Neuron"; date: 
            "2025-05-21- 21"; volume: "113"; issue: "10"; pages: "1507-1524.e17"
COMMENT     Sequence Label: AiP15138: pAAV-AiE0873m_3xC2-minBG-iCre(R297T)...
FEATURES             Location/Qualifiers
     source          1..5199
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      1785..1794
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1797..1817
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2883..2994
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3112..3252
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3112..3241
                     /label=AAV2 ITR
     rep_origin      3327..3782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3414..3433)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3624..3645
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3799..3818)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3918..3940
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3978..3996)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4064..4168
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4169..4237,4238..5029)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4387..4406)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtcatgga tttctccaaa gctttgtagg gcagaactct gaatgtttat
      841 ttctctgatg aatccttaat gaaagtggcc agccatcctg gggagaacaa gccttagttc
      901 ccactgagaa ggatagcaag cagcataaga gtagctgatg tcaaaggcta ttaatgcaaa
      961 tgaaaattat aattcctcat caagatctgc tcccagacag gctaatcatt gtgccacctg
     1021 tttacagaaa cacactgcta atcagtcagt aaaatctttt aagtttctgt gcttctctgt
     1081 gtacgatttt tagcctccat ggatttctcc aaagctttgt agggcagaac tctgaatgtt
     1141 tatttctctg atgaatcctt aatgaaagtg gccagccatc ctggggagaa caagccttag
     1201 ttcccactga gaaggatagc aagcagcata agagtagctg atgtcaaagg ctattaatgc
     1261 aaatgaaaat tataattcct catcaagatc tgctcccaga caggctaatc attgtgccac
     1321 ctgtttacag aaacacactg ctaatcagtc agtaaaatct tttaagtttc tgtgcttctc
     1381 tgtgtacgat ttttagcctc catggatttc tccaaagctt tgtagggcag aactctgaat
     1441 gtttatttct ctgatgaatc cttaatgaaa gtggccagcc atcctgggga gaacaagcct
     1501 tagttcccac tgagaaggat agcaagcagc ataagagtag ctgatgtcaa aggctattaa
     1561 tgcaaatgaa aattataatt cctcatcaag atctgctccc agacaggcta atcattgtgc
     1621 cacctgttta cagaaacaca ctgctaatca gtcagtaaaa tcttttaagt ttctgtgctt
     1681 ctctgtgtac gatttttagc ctcgagctcg ggctgggcat aaaagtcagg gcagagccat
     1741 ctattgctta catttgcttc tgggatccag atctttcgaa gctagccacc atggtgccca
     1801 agaagaagag gaaagtctcc aacctgctga ctgtgcacca aaacctgcct gccctccctg
     1861 tggatgccac ctctgatgaa gtcaggaaga acctgatgga catgttcagg gacaggcagg
     1921 ccttctctga acacacctgg aagatgctcc tgtctgtgtg cagatcctgg gctgcctggt
     1981 gcaagctgaa caacaggaaa tggttccctg ctgaacctga ggatgtgagg gactacctcc
     2041 tgtacctgca agccagaggc ctggctgtga agaccatcca acagcacctg ggccagctca
     2101 acatgctgca caggagatct ggcctgcctc gcccttctga ctccaatgct gtgtccctgg
     2161 tgatgaggag aatcagaaag gagaatgtgg atgctgggga gagagccaag caggccctgg
     2221 cctttgaacg cactgacttt gaccaagtca gatccctgat ggagaactct gacagatgcc
     2281 aggacatcag gaacctggcc ttcctgggca ttgcctacaa caccctgctg cgcattgccg
     2341 aaattgccag aatcagagtg aaggacatct cccgcaccga tggtgggaga atgctgatcc
     2401 acattggcag gaccaagacc ctggtgtcca cagctggtgt ggagaaggcc ctgtccctgg
     2461 gggttaccaa gctggtggag agatggatct ctgtgtctgg tgtggctgat gaccccaaca
     2521 actacctgtt ctgccgggtc agaaagaatg gtgtggctgc cccttctgcc acctcccaac
     2581 tgtccacccg ggccctggaa gggatctttg aggccaccca ccgcctgatc tatggtgcca
     2641 aggatgactc tgggcagaga tacctggcct ggtctggcca ctctgccaga gtgggtgctg
     2701 ccaccgacat ggccagggct ggtgtgtcca tccctgaaat catgcaggct ggtggctgga
     2761 ccaatgtgaa cattgtgatg aactacatca gaaacctgga ctctgagact ggggccatgg
     2821 tgaggctgct cgaagatggg gactgaggcg cgccgaattc aagcttctcg agagatcttc
     2881 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     2941 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3001 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     3061 ttgggaagac aatagcaggc atgagatctc acgtgcggac cgagcggccg caggaacccc
     3121 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     3181 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     3241 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     3301 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     3361 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     3421 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     3481 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     3541 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     3601 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     3661 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     3721 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     3781 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3841 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3901 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3961 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     4021 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4081 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4141 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     4201 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     4261 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     4321 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     4381 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     4441 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4501 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4561 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4621 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4681 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4741 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4801 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4861 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4921 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4981 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     5041 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     5101 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     5161 ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
