LOCUS       sequence-434526-       10456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10456)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-L1CAM-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6061..6096
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6106..6129
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7789..7833
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             7834..7860
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8000..8017)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8006..8117
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8235..8255)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8240..8569
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8420..8555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8482..8501
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8617..8664
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8683..9282
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8683..8702)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9179..9199
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9412..9533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9449..9468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9503..9522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(9586..10377,10378..10446))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10209..10228
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10447..10456,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccat tcagatccct
     2761 gaggaatatg agggccacca cgtgatggaa cctccagtga ttaccgagca gagccctcgg
     2821 agactggtgg tgttccctac cgacgatatc agcctgaagt gcgaggcctc tggaaagccc
     2881 gaggtgcagt tcagatggac cagagatggc gtgcacttca agcccaaaga ggaactgggc
     2941 gtcaccgtgt atcagagccc acacagcggc agctttacca tcaccggcaa caacagcaac
     3001 ttcgcccagc ggttccaggg catctacaga tgctttgcca gcaacaagct gggcaccgcc
     3061 atgagccacg agattagact tatggccgaa ggcgccccta agtggcccaa agaaacagtg
     3121 aagcccgtgg aagtggaaga gggcgaatct gtggtgctgc cctgtaatcc tcctccttcc
     3181 gccgaacctc tgcggatcta ctggatgaac agcaagatcc tgcacatcaa gcaggacgag
     3241 cgcgtgacaa tgggccagaa cggcaacctg tacttcgcca acgtgctgac cagcgacaac
     3301 cacagcgatt acatctgcca cgctcacttc cccggcacca gaaccatcat ccagaaagag
     3361 cccatcgacc tgagagtgaa ggccaccaac agcatgatcg acagaaagcc cagactgctg
     3421 ttccccacca atagcagctc tcacctggtg gctctgcagg gacaacctct ggtgctggaa
     3481 tgtatcgccg agggcttccc cacacctacc attaagtggc tgaggccttc tggccccatg
     3541 cctgccgata gagtgaccta ccagaatcac aacaagaccc tgcagctgct gaaagtgggc
     3601 gaagaggacg atggcgagta cagatgtctg gccgagaata gcctgggaag cgccagacac
     3661 gcctactacg tgacagtgga agccgctcct tactggctgc acaagcctca gtctcacctg
     3721 tatggacctg gcgagacagc cagactggat tgtcaggttc agggcagacc tcagccagaa
     3781 gtgacttgga gaatcaacgg catccctgtg gaagaactgg ccaaggacca gaagtacaga
     3841 atccagagag gcgccctgat cctgagcaac gtgcagccta gcgataccat ggtcacccag
     3901 tgcgaagcca gaaacagaca tggcctgctg ctggccaacg cctacatcta tgttgtgcag
     3961 ctgcccgcca agattctgac cgccgacaat cagacctata tggccgtgca gggaagcacc
     4021 gcctacctgc tgtgtaaagc ttttggcgcc cctgtgccta gcgtgcagtg gctggatgaa
     4081 gatggcacca cagtgctgca ggatgagaga ttcttcccct acgccaatgg caccctgggc
     4141 attagagatc tgcaggccaa cgataccggc agatacttct gcctggccgc caacgatcag
     4201 aacaacgtga ccatcatggc caacctgaaa gtgaaagacg ccacgcagat cacacagggc
     4261 cctagaagca ccatcgagaa gaaaggcagc agagtcacct tcacctgtca agccagcttc
     4321 gaccctagcc tgcagccttc tattacttgg agaggcgacg gcagggacct gcaagagctg
     4381 ggcgatagcg acaagtactt catcgaggac ggccggctgg tcatccacag cctggattac
     4441 agcgaccagg gcaattacag ctgtgtggcc agcaccgagc tggacgttgt ggaaagtaga
     4501 gcacagctgc tggttgtggg aagcccagga cctgttccta gactggtgct gagcgatctg
     4561 catctgctga cacagagcca agtgcgagtg tcttggagcc ctgccgaaga tcacaacgcc
     4621 cctatcgaga agtacgacat cgagttcgag gacaaagaga tggcccctga gaagtggtac
     4681 agcctgggca aagtgcccgg caaccagacc tctacaacac tgaagctgag cccctacgtg
     4741 cactacacct tcagagtgac cgccatcaac aaatacggcc ctggcgagcc ttctccagtg
     4801 tccgaaacag tggttacacc tgaggccgct ccagagaaga accccgtgga tgtgaaaggc
     4861 gagggcaacg aaaccaccaa catggtcatc acctggaagc ccctgcggtg gatggattgg
     4921 aatgcccctc aggtgcagta cagggttcag tggcgaccac agggaacaag aggaccctgg
     4981 caagagcaga tcgtgtccga tcctttcctg gtcgtgtcca acaccagcac cttcgtgccc
     5041 tacgagatta aggtgcaggc cgtgaattcc caaggcaagg gacctgagcc tcaagtgaca
     5101 atcggctaca gcggcgagga ctaccctcag gccattcctg agctggaagg catcgagatc
     5161 ctgaactcta gcgccgtgct tgtgaagtgg cggcctgtgg atctggccca agtgaaagga
     5221 cacctgagag gctacaatgt gacctattgg agagagggca gccagcggaa gcacagcaag
     5281 agacacatcc acaaggatca tgtggtggtg cctgccaaca ccacctccgt gattctgagc
     5341 ggcctgaggc catacagcag ctaccatctg gaagtgcagg ctttcaacgg cagaggctct
     5401 ggccctgcca gcgagtttac attttctacc cctgagggcg tgccaggaca ccctgaagca
     5461 ctgcatctgg aatgccagag caacacctct ctgctgctga gatggcagcc tcctctgtct
     5521 cacaatggcg tgctgacagg ctacgtgctg agctatcacc ctctggacga aggcggaaag
     5581 ggccagctga gcttcaatct gagagatccc gagctgcgga cccacaacct gacagatttg
     5641 tctccccacc tgagataccg gttccagctg caagccacta ccaaagaagg accaggcgag
     5701 gccatcgtca gagaaggtgg aacaatggcc ctgagcggca tcagcgactt tggcaatatt
     5761 tctgccactg ccggcgagaa ctacagcgtg gtgtcttggg ttccaaaaga gggccagtgc
     5821 aacttccggt tccacatcct gtttaaggcc ctgggagaag agaaaggcgg agccagtctg
     5881 agccctcagt acgtgtccta caaccagagc agctacaccc agtgggatct gcagcctgac
     5941 accgactacg agatccacct gttcaaagaa cggatgttca gacaccagat ggccgtcaag
     6001 accaacggaa ctggcagagt tagactgcct cctgccggct ttgccaccga aggcgcgccg
     6061 gaagatcaag ttgatccaag attgattgat ggcaagggcg gatctctgga ggtgctgttc
     6121 cagggaccag gcggaagcgg caatcccggc ggcgatctgg ccccccagat gctgcgcgag
     6181 ctgcaggaga ccaatgccgc cctgcaggac gtgcgcgagc tgctgcgcca ccgcgtgaag
     6241 gagatcacct tcctgaagaa caccgtgatg gagtgcgacg cctgcggtat gcagccctct
     6301 agaggctccg gctccatcat cccagttgag gaggagaacc cggacttctg gaaccgcgag
     6361 gcagccgagg ccctgggtgc cgccaagaag ctgcagcctg cacagacagc cgccaagaac
     6421 ctcatcatct tcctgggcga tgggatgggg gtgtctacgg tgacagctgc caggatccta
     6481 aaagggcaga agaaggacaa actggggcct gagatacccc tggccatgga ccgcttccca
     6541 tatgtggctc tgtccaagac atacaatgta gacaaacatg tgccagacag tggagccaca
     6601 gccacggcct acctgtgcgg ggtcaagggc aacttccaga ccattggctt gagtgcagcc
     6661 gcccgcttta accagtgcaa cacgacacgc ggcaacgagg tcatctccgt gatgaatcgg
     6721 gccaagaaag cagggaagtc agtgggagtg gtaaccacca cacgagtgca gcacgcctcg
     6781 ccagccggca cctacgccca cacggtgaac cgcaactggt actcggacgc cgacgtgcct
     6841 gcctcggccc gccaggaggg gtgccaggac atcgctacgc agctcatctc caacatggac
     6901 attgacgtga tcctaggtgg aggccgaaag tacatgtttc gcatgggaac cccagaccct
     6961 gagtacccag atgactacag ccaaggtggg accaggctgg acgggaagaa tctggtgcag
     7021 gaatggctgg cgaagcgcca gggtgcccgg tatgtgtgga accgcactga gctcatgcag
     7081 gcttccctgg acccgtctgt gacccatctc atgggtctct ttgagcctgg agacatgaaa
     7141 tacgagatcc accgagactc cacactggac ccctccctga tggagatgac agaggctgcc
     7201 ctgcgcctgc tgagcaggaa cccccgcggc ttcttcctct tcgtggaggg tggtcgcatc
     7261 gaccatggtc atcatgaaag cagggcttac cgggcactga ctgagacgat catgttcgac
     7321 gacgccattg agagggcggg ccagctcacc agcgaggagg acacgctgag cctcgtcact
     7381 gccgaccact cccacgtctt ctccttcgga ggctaccccc tgcgagggag ctccatcttc
     7441 gggctggccc ctggcaaggc ccgggacagg aaggcctaca cggtcctcct atacggaaac
     7501 ggtccaggct atgtgctcaa ggacggcgcc cggccggatg ttaccgagag cgagagcggg
     7561 agccccgagt atcggcagca gtcagcagtg cccctggacg aagagaccca cgcaggcgag
     7621 gacgtggcgg tgttcgcgcg cggcccgcag gcgcacctgg ttcacggcgt gcaggagcag
     7681 accttcatag cgcacgtcat ggccttcgcc gcctgcctgg agccctacac cgcctgcgac
     7741 ctggcgcccc ccgccggcac caccgacgcc gcgcacccgg gtggcggagg actgaacgac
     7801 atcttcgagg ctcagaaaat cgaatggcac gaacatcacc atcaccatca ccatcaccat
     7861 taaggccggc ctgtagtcac tggcacaacg cgttacccgg taagccaatc gggtatacac
     7921 ggtcgtcata ctgcagacag ggttcttcta ctttgcaaga tagtcttgag tagtaaaata
     7981 aatagataga gaaaatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     8041 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     8101 aatgaggaaa ttgcatcaca acactcaacc ctatctcggt ctattctttt gatttataag
     8161 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8221 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8281 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8341 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8401 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8461 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8521 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8581 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8641 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatgaccga gtacaagccc
     8701 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     8761 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     8821 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     8881 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     8941 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     9001 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     9061 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     9121 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacatc cgcgccccgc
     9181 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     9241 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacacgtgct acgagatttc
     9301 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     9361 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     9421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     9481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     9541 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcattacc aatgcttaat
     9601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagcg ctgcgatgat
     9721 accgcgagaa ccacgctcac cggctccgga tttatcagca ataaaccagc cagccggaag
     9781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccatcgc
     9901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
    10021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
    10081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
    10141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
    10201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
    10261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
    10321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10441 actcatattc ttcctt
//
