LOCUS       sequence-434655-        2899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mitochondrial cytosine base editor arm in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Castillo SR, Simone BW, Clark KJ, Devaux P, Ekker SC
  TITLE     Unconstrained Precision Mitochondrial Genome Editing with 
            alphaDdCBEs.
  JOURNAL   Hum Gene Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073.
  PUBMED    39212664
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073."
COMMENT     Sequence Label: ND2 alphaDdCBE C1
FEATURES             Location/Qualifiers
     source          1..2899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..78
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2497..2748
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggcctccg ttctgacccc gctgctgctg cgtggcctga ccggctccgc tcgtcgtctg
       61 ccagttccgc gtgcgaaaat ccattccctg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg acatcgcgga tctgcgtacc
      181 ctgggttaca gccagcagca gcaggagaag atcaagccaa aggtgcgcag caccgtggcc
      241 cagcaccatg aagctctggt gggtcacggc ttcacccacg cgcacatcgt ggctctgagc
      301 cagcacccag ccgcgctggg taccgtggcc gtgaaatatc aggacatgat tgctgccctg
      361 ccagaggcta cccatgaagc gattgtgggt gtgggcaaga gaggagccgg tgctcgtgcg
      421 ctggaggccc tgctgaccgt ggccggtgaa ctgcgtggcc cgccgctgca gctggatacc
      481 ggccagctgc tgaaaatcgc gaaacgtggc ggtgtgaccg ctgtggaagc tgtgcatgcc
      541 tggcgtaatg ctctgaccgg tgcccccctg aacctgaccc cggaccaagt ggtggctatc
      601 gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
      661 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa cggtggcggc
      721 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
      781 accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc gctcgaaacg
      841 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
      901 gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
      961 gtgctgtgcc aggaccatgg cctgactccg gaccaagtgg tggctatcgc cagccacgat
     1021 ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat
     1081 ggcctgactc cggaccaagt ggtggctatc gccagccacg atggcggcaa gcaagcgctc
     1141 gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa
     1201 gtggtggcta tcgccagcaa cattggcggc aagcaagcgc tcgaaacggt gcagcggctg
     1261 ttgccggtgc tgtgccagga ccatggcctc acacccgatc aggtcgtggc tatcgccagc
     1321 aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag
     1381 gaccatggcc tgactccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa
     1441 gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg
     1501 gaccaagtgg tggctatcgc cagcaacatt ggcggcaagc aagcgctcga aacggtgcag
     1561 cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc
     1621 gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
     1681 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa cattggcggc
     1741 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
     1801 accccggacc aagtggtggc tatcgccagc aacaatggcg gcaagcaagc gctcgaaacg
     1861 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgactccgga ccaagtggtg
     1921 gctatcgcca gccacgatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
     1981 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacatt
     2041 ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat
     2101 ggcctgaccc cggaccaagt ggtggctatc gccagcaaca atggcggcaa gcaagcgctc
     2161 gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa
     2221 gtggtggcta tcgccagcaa caatggcggc aagcaagcgc tcgaaagcat tgtggcccag
     2281 ctgagccggc ctgatccggc gttggccgcg ttgaccaacg atcacctggt ggccctggcc
     2341 tgcctgggcg gtcgtccggc cctggatgcg gtgaagaaag gcctgggtgg atccgccatt
     2401 ccagtgaagc gcggcgctac cggtgaaacc aaagtgttta ccggtaacag caacagcccg
     2461 aagagcccga ccaaaggcgg ttgctctggc ggctcaacta atctgagcga catcattgag
     2521 aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg
     2581 gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag
     2641 tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc
     2701 ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgtgata aggggtcttt
     2761 gtcctctgta ctgtctctct ccttgcccct aacccaaaaa gcttcatttt tctgtgtagg
     2821 ctgcacaaga gccttgattg aagatatatt ctttctgaac agtatttaag gtttccaata
     2881 aaatgtacac ccctcagaa
//
