LOCUS       sequence-434681-        3019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses monomeric mitochondrial cytosine base editor in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Castillo SR, Simone BW, Clark KJ, Devaux P, Ekker SC
  TITLE     Unconstrained Precision Mitochondrial Genome Editing with 
            alphaDdCBEs.
  JOURNAL   Hum Gene Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073.
  PUBMED    39212664
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2024 Sep 24. doi: 10.1089/hum.2024.073."
COMMENT     Sequence Label: TC mDdCBE T2
FEATURES             Location/Qualifiers
     source          1..3019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..78
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     CDS             91..156
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2617..2868
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
ORIGIN
        1 atggcctccg ttctgacccc gctgctgctg cgtggcctga ccggctccgc tcgtcgtctg
       61 ccagttccgc gtgcgaaaat ccattccctg gactacaaag accatgacgg tgattataaa
      121 gatcatgaca tcgattacaa ggatgacgat gacaagatgg acatcgcgga tctgcgtacc
      181 ctgggttaca gccagcagca gcaggagaag atcaagccaa aggtgcgcag caccgtggcc
      241 cagcaccatg aagctctggt gggtcacggc ttcacccacg cgcacatcgt ggctctgagc
      301 cagcacccag ccgcgctggg taccgtggcc gtgaaatatc aggacatgat tgctgccctg
      361 ccagaggcta cccatgaagc gattgtgggt gtgggcaagc agtggagcgg tgctcgtgcg
      421 ctggaggccc tgctgaccgt ggccggtgaa ctgcgtggcc cgccgctgca gctggatacc
      481 ggccagctgc tgaaaatcgc gaaacgtggc ggtgtgaccg ctgtggaagc tgtgcatgcc
      541 tggcgtaatg ctctgaccgg tgcccccctg aacctgaccc cggaccaagt ggtggctatc
      601 gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
      661 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa cggtggcggc
      721 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
      781 accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg
      841 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
      901 gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
      961 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacatt
     1021 ggcggcaagc aagcgctcga aacggtgcag cggctgttgc cggtgctgtg ccaggaccat
     1081 ggcctgaccc cggaccaagt ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc
     1141 gaaacggtgc agcggctgtt gccggtgctg tgccaggacc atggcctgac cccggaccaa
     1201 gtggtggcta tcgccagcaa cggtggcggc aagcaagcgc tcgaaacggt gcagcggctg
     1261 ttgccggtgc tgtgccagga ccatggcctc acacccgatc aggtcgtggc tatcgccagc
     1321 aacggtggcg gcaagcaagc gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag
     1381 gaccatggcc tgaccccgga ccaagtggtg gctatcgcca gcaacaatgg cggcaagcaa
     1441 gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg
     1501 gaccaagtgg tggctatcgc cagccacgat ggcggcaagc aagcgctcga aacggtgcag
     1561 cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc
     1621 gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
     1681 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa cattggcggc
     1741 aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga ccatggcctg
     1801 accccggacc aagtggtggc tatcgccagc aacattggcg gcaagcaagc gctcgaaacg
     1861 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
     1921 gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgttgccg
     1981 gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcgc cagcaacggt
     2041 ggcggcaagc aagcgctcga aagcattgtg gcccagctga gccggcctga tccggcgttg
     2101 gccgcgttga ccaacgatca cctggtggcc ctggcctgcc tgggcggtcg tccggccctg
     2161 gatgcggtga agaaaggcct gggtggatcc ggcagctacg ccctgggtcc gtatcagatt
     2221 agcgccccgc agctgccagc atacaatggt cagaccgtgg gtaccttcta ctatgtgaac
     2281 gacgctggcg gtctggaggg caaggtgttt agcagcggcg gtccaacccc gtacccaaac
     2341 tatgccaatg ccggtcatgt ggagagtcag agcgccctgt tcatgcgtga taacggcatc
     2401 agcgagggtc tggtgttcca caacaacccg gaaggcacct gcggtttttg cgtgaacatg
     2461 accgagaccc tgctgccgga aaacgcgaaa atgaccgtgg tgccgccgga aggtgtcatt
     2521 ccagtgaagc gcggcgctac cggtgaaacc aaagtgttta ccggtaacag caacggcccg
     2581 aagagcccga ccaaaggcgg ttgctctggc ggctcaacta atctgagcga catcattgag
     2641 aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg
     2701 gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag
     2761 tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc
     2821 ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgtgata aggggtcttt
     2881 gtcctctgta ctgtctctct ccttgcccct aacccaaaaa gcttcatttt tctgtgtagg
     2941 ctgcacaaga gccttgattg aagatatatt ctttctgaac agtatttaag gtttccaata
     3001 aaatgtacac ccctcagaa
//
